Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacity
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquaculture.2022.738976 http://hdl.handle.net/11449/246151 |
Resumo: | This study evaluated the effects of dietary glutamine (Gln) supplementation on growth, intestinal morphology, and health status of Nile tilapia juveniles. Four hundred forty GIFT tilapia juveniles (4.4 ± 0.1 g) were randomly distributed in 40 tanks (250-L) and fed five practical diets supplemented with graded levels of L-glutamine (0, 0.5, 1.0, 1.5, and 2.0% Gln) with eight replicate tanks/diet. The experimental diets were formulated to contain 29% digestible protein and 14.5 MJ kg−1 digestible energy. After 90 days of feeding, growth performance, intestinal morphology, and body proximate composition were evaluated. Additionally, eight fish per treatment were sampled for the assessment of hematological profile, antioxidant capacity and innate immune responses. Glutamine supplementation improved relative weight and length of the intestine, as well as villus length and villus:crypt ratio, which could be related to better feed efficiency observed in fish fed Gln-supplemented diets. Final body weight and weight gain also were positively affected by dietary Gln supplementation. Fish fed Gln-supplemented diets showed increased glutathione (GSH) concentrations in the intestine. However, Gln supplementation had no significant effects on hematological and biochemical parameters and presented limited effects on innate immune responses. In summary, dietary Gln supplementation improved growth, feed efficiency, and promoted Nile tilapia intestinal development and function. Based on polynomial regression analysis of growth performance parameters, the recommended level of Gln supplementation in diets for juvenile Nile tilapia was estimated to be 1.33% of dry weight. These results also imply that higher levels of Gln supplementation may be required to support enhanced immune responses and antioxidant capacity, but further studies are necessary to evaluate these latter notions. |
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Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacityAmino acidGrowthIntestinal functionL-glutamineNile tilapiaThis study evaluated the effects of dietary glutamine (Gln) supplementation on growth, intestinal morphology, and health status of Nile tilapia juveniles. Four hundred forty GIFT tilapia juveniles (4.4 ± 0.1 g) were randomly distributed in 40 tanks (250-L) and fed five practical diets supplemented with graded levels of L-glutamine (0, 0.5, 1.0, 1.5, and 2.0% Gln) with eight replicate tanks/diet. The experimental diets were formulated to contain 29% digestible protein and 14.5 MJ kg−1 digestible energy. After 90 days of feeding, growth performance, intestinal morphology, and body proximate composition were evaluated. Additionally, eight fish per treatment were sampled for the assessment of hematological profile, antioxidant capacity and innate immune responses. Glutamine supplementation improved relative weight and length of the intestine, as well as villus length and villus:crypt ratio, which could be related to better feed efficiency observed in fish fed Gln-supplemented diets. Final body weight and weight gain also were positively affected by dietary Gln supplementation. Fish fed Gln-supplemented diets showed increased glutathione (GSH) concentrations in the intestine. However, Gln supplementation had no significant effects on hematological and biochemical parameters and presented limited effects on innate immune responses. In summary, dietary Gln supplementation improved growth, feed efficiency, and promoted Nile tilapia intestinal development and function. Based on polynomial regression analysis of growth performance parameters, the recommended level of Gln supplementation in diets for juvenile Nile tilapia was estimated to be 1.33% of dry weight. These results also imply that higher levels of Gln supplementation may be required to support enhanced immune responses and antioxidant capacity, but further studies are necessary to evaluate these latter notions.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Melhoramento e Nutrição Animal UNESP Universidade Estadual Paulista “Júlio de Mesquita Filho”, SPDepartamento de Zootecnia UEPG Universidade Estadual de Ponta Grossa, PRThad Cochran National Warmwater Aquaculture Center Mississippi Agriculture and Forestry Experiment Station Mississippi State UniversityDepartment of Wildlife Fisheries and Aquaculture Mississippi State UniversityDepartment of Ecology and Conservation Biology Texas A&M AgriLife Research Texas A&M University SystemDepartamento de Melhoramento e Nutrição Animal UNESP Universidade Estadual Paulista “Júlio de Mesquita Filho”, SPUniversidade Estadual Paulista (UNESP)Universidade Estadual de Ponta Grossa (UEPG)Mississippi State UniversityTexas A&M University SystemCarvalho, Pedro L.P.F. [UNESP]Xavier, William dos S. [UNESP]Guimarães, Matheus G. [UNESP]Rodrigues, Edgar J.D. [UNESP]Furuya, Wilson M.Yamamoto, Fernando Y.Pezzato, Luiz E. [UNESP]Gatlin, Delbert M.Barros, Margarida M. [UNESP]2023-07-29T12:33:05Z2023-07-29T12:33:05Z2023-01-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.aquaculture.2022.738976Aquaculture, v. 563.0044-8486http://hdl.handle.net/11449/24615110.1016/j.aquaculture.2022.7389762-s2.0-85140490497Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquacultureinfo:eu-repo/semantics/openAccess2023-07-29T12:33:06Zoai:repositorio.unesp.br:11449/246151Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:33:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacity |
title |
Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacity |
spellingShingle |
Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacity Carvalho, Pedro L.P.F. [UNESP] Amino acid Growth Intestinal function L-glutamine Nile tilapia |
title_short |
Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacity |
title_full |
Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacity |
title_fullStr |
Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacity |
title_full_unstemmed |
Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacity |
title_sort |
Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacity |
author |
Carvalho, Pedro L.P.F. [UNESP] |
author_facet |
Carvalho, Pedro L.P.F. [UNESP] Xavier, William dos S. [UNESP] Guimarães, Matheus G. [UNESP] Rodrigues, Edgar J.D. [UNESP] Furuya, Wilson M. Yamamoto, Fernando Y. Pezzato, Luiz E. [UNESP] Gatlin, Delbert M. Barros, Margarida M. [UNESP] |
author_role |
author |
author2 |
Xavier, William dos S. [UNESP] Guimarães, Matheus G. [UNESP] Rodrigues, Edgar J.D. [UNESP] Furuya, Wilson M. Yamamoto, Fernando Y. Pezzato, Luiz E. [UNESP] Gatlin, Delbert M. Barros, Margarida M. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Estadual de Ponta Grossa (UEPG) Mississippi State University Texas A&M University System |
dc.contributor.author.fl_str_mv |
Carvalho, Pedro L.P.F. [UNESP] Xavier, William dos S. [UNESP] Guimarães, Matheus G. [UNESP] Rodrigues, Edgar J.D. [UNESP] Furuya, Wilson M. Yamamoto, Fernando Y. Pezzato, Luiz E. [UNESP] Gatlin, Delbert M. Barros, Margarida M. [UNESP] |
dc.subject.por.fl_str_mv |
Amino acid Growth Intestinal function L-glutamine Nile tilapia |
topic |
Amino acid Growth Intestinal function L-glutamine Nile tilapia |
description |
This study evaluated the effects of dietary glutamine (Gln) supplementation on growth, intestinal morphology, and health status of Nile tilapia juveniles. Four hundred forty GIFT tilapia juveniles (4.4 ± 0.1 g) were randomly distributed in 40 tanks (250-L) and fed five practical diets supplemented with graded levels of L-glutamine (0, 0.5, 1.0, 1.5, and 2.0% Gln) with eight replicate tanks/diet. The experimental diets were formulated to contain 29% digestible protein and 14.5 MJ kg−1 digestible energy. After 90 days of feeding, growth performance, intestinal morphology, and body proximate composition were evaluated. Additionally, eight fish per treatment were sampled for the assessment of hematological profile, antioxidant capacity and innate immune responses. Glutamine supplementation improved relative weight and length of the intestine, as well as villus length and villus:crypt ratio, which could be related to better feed efficiency observed in fish fed Gln-supplemented diets. Final body weight and weight gain also were positively affected by dietary Gln supplementation. Fish fed Gln-supplemented diets showed increased glutathione (GSH) concentrations in the intestine. However, Gln supplementation had no significant effects on hematological and biochemical parameters and presented limited effects on innate immune responses. In summary, dietary Gln supplementation improved growth, feed efficiency, and promoted Nile tilapia intestinal development and function. Based on polynomial regression analysis of growth performance parameters, the recommended level of Gln supplementation in diets for juvenile Nile tilapia was estimated to be 1.33% of dry weight. These results also imply that higher levels of Gln supplementation may be required to support enhanced immune responses and antioxidant capacity, but further studies are necessary to evaluate these latter notions. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:33:05Z 2023-07-29T12:33:05Z 2023-01-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquaculture.2022.738976 Aquaculture, v. 563. 0044-8486 http://hdl.handle.net/11449/246151 10.1016/j.aquaculture.2022.738976 2-s2.0-85140490497 |
url |
http://dx.doi.org/10.1016/j.aquaculture.2022.738976 http://hdl.handle.net/11449/246151 |
identifier_str_mv |
Aquaculture, v. 563. 0044-8486 10.1016/j.aquaculture.2022.738976 2-s2.0-85140490497 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046080524320768 |