LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10616-016-9998-6 http://hdl.handle.net/11449/173135 |
Resumo: | Brazilian flora biodiversity has been widely investigated to identify effective and safe phytotherapeutic compounds. Among the investigated plant species, the Byrsonima genus exhibits promising biological activities. This study aimed at evaluating the cytotoxicity of B. correifolia, B. verbascifolia, B. fagifolia and B. intermedia extracts using different assays in two cell lines (primary gastric and HepG2 cells). The different extract concentrations effects on cell viability were assayed using the MTT, aquabluer, neutral red and LDH assays. Non-cytotoxic concentrations were selected to generate cell proliferation curves and to assess cell cycle kinetics by flow cytometry. Byrsonima extracts differentially affected cell viability depending on the metabolic cellular state and the biological parameter evaluated. B. fagifolia and B. intermedia extracts exhibited lower cytotoxic effects than B. correifolia and B. verbascifolia in all assays. The results obtained with LDH and flow cytometry assays were more reliable, suggesting that they can be useful in the screening for herbal medicine and to further characterize these extracts as phytotherapeutic compounds. |
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LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compoundsAntioxidantByrsonima spCytostaticCytotoxicityFlow cytometryHerbal medicineBrazilian flora biodiversity has been widely investigated to identify effective and safe phytotherapeutic compounds. Among the investigated plant species, the Byrsonima genus exhibits promising biological activities. This study aimed at evaluating the cytotoxicity of B. correifolia, B. verbascifolia, B. fagifolia and B. intermedia extracts using different assays in two cell lines (primary gastric and HepG2 cells). The different extract concentrations effects on cell viability were assayed using the MTT, aquabluer, neutral red and LDH assays. Non-cytotoxic concentrations were selected to generate cell proliferation curves and to assess cell cycle kinetics by flow cytometry. Byrsonima extracts differentially affected cell viability depending on the metabolic cellular state and the biological parameter evaluated. B. fagifolia and B. intermedia extracts exhibited lower cytotoxic effects than B. correifolia and B. verbascifolia in all assays. The results obtained with LDH and flow cytometry assays were more reliable, suggesting that they can be useful in the screening for herbal medicine and to further characterize these extracts as phytotherapeutic compounds.Department of General Biology State University of Londrina, PR 445 Km 380, s/n - Campus UniversitárioDepartment of Biological Sciences Faculty of Pharmaceutical Sciences of Araraquara São Paulo State University “Júlio de Mesquita Filho”School of Arts Science and Humanities São Paulo UniversityInstitute of Biosciences UNESP- Paulista State University, Coastal Campus of São VicenteInstituto de Investigaciones Biológicas Clemente EstableDepartment of Biological Sciences Faculty of Pharmaceutical Sciences of Araraquara São Paulo State University “Júlio de Mesquita Filho”Institute of Biosciences UNESP- Paulista State University, Coastal Campus of São VicenteUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Instituto de Investigaciones Biológicas Clemente EstableSpecian, Ana Flávia L.Serpeloni, Juliana M. [UNESP]Tuttis, KatiuskaRibeiro, Diego L.Cilião, Heloísa L.Varanda, Eliana A. [UNESP]Sannomiya, MiriamMartinez-Lopez, WilnerVilegas, Wagner [UNESP]Cólus, Ilce M. S.2018-12-11T17:03:48Z2018-12-11T17:03:48Z2016-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2729-2744application/pdfhttp://dx.doi.org/10.1007/s10616-016-9998-6Cytotechnology, v. 68, n. 6, p. 2729-2744, 2016.1573-07780920-9069http://hdl.handle.net/11449/17313510.1007/s10616-016-9998-62-s2.0-849763180332-s2.0-84976318033.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytotechnology0,5190,519info:eu-repo/semantics/openAccess2024-06-24T13:08:24Zoai:repositorio.unesp.br:11449/173135Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:33:57.918911Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds |
title |
LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds |
spellingShingle |
LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds Specian, Ana Flávia L. Antioxidant Byrsonima sp Cytostatic Cytotoxicity Flow cytometry Herbal medicine |
title_short |
LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds |
title_full |
LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds |
title_fullStr |
LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds |
title_full_unstemmed |
LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds |
title_sort |
LDH, proliferation curves and cell cycle analysis are the most suitable assays to identify and characterize new phytotherapeutic compounds |
author |
Specian, Ana Flávia L. |
author_facet |
Specian, Ana Flávia L. Serpeloni, Juliana M. [UNESP] Tuttis, Katiuska Ribeiro, Diego L. Cilião, Heloísa L. Varanda, Eliana A. [UNESP] Sannomiya, Miriam Martinez-Lopez, Wilner Vilegas, Wagner [UNESP] Cólus, Ilce M. S. |
author_role |
author |
author2 |
Serpeloni, Juliana M. [UNESP] Tuttis, Katiuska Ribeiro, Diego L. Cilião, Heloísa L. Varanda, Eliana A. [UNESP] Sannomiya, Miriam Martinez-Lopez, Wilner Vilegas, Wagner [UNESP] Cólus, Ilce M. S. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Instituto de Investigaciones Biológicas Clemente Estable |
dc.contributor.author.fl_str_mv |
Specian, Ana Flávia L. Serpeloni, Juliana M. [UNESP] Tuttis, Katiuska Ribeiro, Diego L. Cilião, Heloísa L. Varanda, Eliana A. [UNESP] Sannomiya, Miriam Martinez-Lopez, Wilner Vilegas, Wagner [UNESP] Cólus, Ilce M. S. |
dc.subject.por.fl_str_mv |
Antioxidant Byrsonima sp Cytostatic Cytotoxicity Flow cytometry Herbal medicine |
topic |
Antioxidant Byrsonima sp Cytostatic Cytotoxicity Flow cytometry Herbal medicine |
description |
Brazilian flora biodiversity has been widely investigated to identify effective and safe phytotherapeutic compounds. Among the investigated plant species, the Byrsonima genus exhibits promising biological activities. This study aimed at evaluating the cytotoxicity of B. correifolia, B. verbascifolia, B. fagifolia and B. intermedia extracts using different assays in two cell lines (primary gastric and HepG2 cells). The different extract concentrations effects on cell viability were assayed using the MTT, aquabluer, neutral red and LDH assays. Non-cytotoxic concentrations were selected to generate cell proliferation curves and to assess cell cycle kinetics by flow cytometry. Byrsonima extracts differentially affected cell viability depending on the metabolic cellular state and the biological parameter evaluated. B. fagifolia and B. intermedia extracts exhibited lower cytotoxic effects than B. correifolia and B. verbascifolia in all assays. The results obtained with LDH and flow cytometry assays were more reliable, suggesting that they can be useful in the screening for herbal medicine and to further characterize these extracts as phytotherapeutic compounds. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 2018-12-11T17:03:48Z 2018-12-11T17:03:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10616-016-9998-6 Cytotechnology, v. 68, n. 6, p. 2729-2744, 2016. 1573-0778 0920-9069 http://hdl.handle.net/11449/173135 10.1007/s10616-016-9998-6 2-s2.0-84976318033 2-s2.0-84976318033.pdf |
url |
http://dx.doi.org/10.1007/s10616-016-9998-6 http://hdl.handle.net/11449/173135 |
identifier_str_mv |
Cytotechnology, v. 68, n. 6, p. 2729-2744, 2016. 1573-0778 0920-9069 10.1007/s10616-016-9998-6 2-s2.0-84976318033 2-s2.0-84976318033.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cytotechnology 0,519 0,519 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2729-2744 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129438157635584 |