Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial dna
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-4685-GMB-2020-0249 http://hdl.handle.net/11449/207489 |
Resumo: | The main objective of this study was to estimate the genetic diversity levels and haplotype traceability in pacu Piaractus mesopotamicus from the breeding program located in Brazil by analyses of the mitochondrial DNA control region (mtDNA). Moreover, broodstocks from eight commercial fish farms were used for comparative evaluation, four from Brazil (Br1-Br4) and four from Argentina (Ar1-Ar4). The descriptive results revealed 47 polymorphic sites and 51 mutations, which evidenced 34 haplotypes. Ten haplotypes were shared among fish farms and 24 were exclusive. The nucleotide diversity (π) ranged from 0.00031 to 0.01462 and haplotype diversity (Hd) from 0.125 to 0.868. The analysis of molecular variance (AMOVA) indicated high structure present in the analyzed stocks (FST = 0.13356 and ФST = 0.52707). The genetic diversity was high in most of the commercial broodstocks, especially those from Brazil. We observed seven haplotypes in the genetic breeding population, of which four were exclusive and three shared among the commercial fish farms. The genetic diversity was moderate (π = 0.00265 and Hd = 0.424) and considered appropriated for this breeding population of pacu. Our results provide support for the genetic diversity maintenance and mtDNA traceability of pacu commercial broodstocks. |
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Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial dnaAquacultureControl-regionGenetic breedingThe main objective of this study was to estimate the genetic diversity levels and haplotype traceability in pacu Piaractus mesopotamicus from the breeding program located in Brazil by analyses of the mitochondrial DNA control region (mtDNA). Moreover, broodstocks from eight commercial fish farms were used for comparative evaluation, four from Brazil (Br1-Br4) and four from Argentina (Ar1-Ar4). The descriptive results revealed 47 polymorphic sites and 51 mutations, which evidenced 34 haplotypes. Ten haplotypes were shared among fish farms and 24 were exclusive. The nucleotide diversity (π) ranged from 0.00031 to 0.01462 and haplotype diversity (Hd) from 0.125 to 0.868. The analysis of molecular variance (AMOVA) indicated high structure present in the analyzed stocks (FST = 0.13356 and ФST = 0.52707). The genetic diversity was high in most of the commercial broodstocks, especially those from Brazil. We observed seven haplotypes in the genetic breeding population, of which four were exclusive and three shared among the commercial fish farms. The genetic diversity was moderate (π = 0.00265 and Hd = 0.424) and considered appropriated for this breeding population of pacu. Our results provide support for the genetic diversity maintenance and mtDNA traceability of pacu commercial broodstocks.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Centro de AquiculturaUniversidad Nacional de Rosario Facultad de Ciencias Bioquímicas y Farmacéuticas-Ministerio de Ciencia Tecnología e Innovación productiva de Santa Fe Centro Científico y Tecnológico Acuario del Río ParanáUniversidade Federal de São Paulo (UNIFESP) Instituto do MarUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Faculdade de CiênciasUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Centro de AquiculturaUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Faculdade de CiênciasCAPES: 001FAPESP: 15/14185-8FAPESP: 2014/03772-7CNPq: 311559/2018-2CNPq: 446779/2014-8Universidade Estadual Paulista (Unesp)Centro Científico y Tecnológico Acuario del Río ParanáUniversidade Federal de São Paulo (UNIFESP)de Freitas, Milena V. [UNESP]Ariede, Raquel B. [UNESP]Hata, Milene E. [UNESP]Mastrochirico-Filho, Vito A. [UNESP]Pazo, Felipe DelVillanova, Gabriela V.Mendonça, Fernando F.Porto-Foresti, Fábio [UNESP]Hashimoto, Diogo T. [UNESP]2021-06-25T10:56:04Z2021-06-25T10:56:04Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1678-4685-GMB-2020-0249Genetics and Molecular Biology, v. 44, n. 1, 2021.1678-46851415-4757http://hdl.handle.net/11449/20748910.1590/1678-4685-GMB-2020-0249S1415-475720210001002022-s2.0-85102931270S1415-47572021000100202.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biologyinfo:eu-repo/semantics/openAccess2024-04-09T15:29:47Zoai:repositorio.unesp.br:11449/207489Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:29:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial dna |
title |
Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial dna |
spellingShingle |
Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial dna de Freitas, Milena V. [UNESP] Aquaculture Control-region Genetic breeding |
title_short |
Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial dna |
title_full |
Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial dna |
title_fullStr |
Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial dna |
title_full_unstemmed |
Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial dna |
title_sort |
Haplotypes traceability and genetic variability of the breeding population of pacu (Piaractus mesopotamicus) revealed by mitochondrial dna |
author |
de Freitas, Milena V. [UNESP] |
author_facet |
de Freitas, Milena V. [UNESP] Ariede, Raquel B. [UNESP] Hata, Milene E. [UNESP] Mastrochirico-Filho, Vito A. [UNESP] Pazo, Felipe Del Villanova, Gabriela V. Mendonça, Fernando F. Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo T. [UNESP] |
author_role |
author |
author2 |
Ariede, Raquel B. [UNESP] Hata, Milene E. [UNESP] Mastrochirico-Filho, Vito A. [UNESP] Pazo, Felipe Del Villanova, Gabriela V. Mendonça, Fernando F. Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo T. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Centro Científico y Tecnológico Acuario del Río Paraná Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
de Freitas, Milena V. [UNESP] Ariede, Raquel B. [UNESP] Hata, Milene E. [UNESP] Mastrochirico-Filho, Vito A. [UNESP] Pazo, Felipe Del Villanova, Gabriela V. Mendonça, Fernando F. Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo T. [UNESP] |
dc.subject.por.fl_str_mv |
Aquaculture Control-region Genetic breeding |
topic |
Aquaculture Control-region Genetic breeding |
description |
The main objective of this study was to estimate the genetic diversity levels and haplotype traceability in pacu Piaractus mesopotamicus from the breeding program located in Brazil by analyses of the mitochondrial DNA control region (mtDNA). Moreover, broodstocks from eight commercial fish farms were used for comparative evaluation, four from Brazil (Br1-Br4) and four from Argentina (Ar1-Ar4). The descriptive results revealed 47 polymorphic sites and 51 mutations, which evidenced 34 haplotypes. Ten haplotypes were shared among fish farms and 24 were exclusive. The nucleotide diversity (π) ranged from 0.00031 to 0.01462 and haplotype diversity (Hd) from 0.125 to 0.868. The analysis of molecular variance (AMOVA) indicated high structure present in the analyzed stocks (FST = 0.13356 and ФST = 0.52707). The genetic diversity was high in most of the commercial broodstocks, especially those from Brazil. We observed seven haplotypes in the genetic breeding population, of which four were exclusive and three shared among the commercial fish farms. The genetic diversity was moderate (π = 0.00265 and Hd = 0.424) and considered appropriated for this breeding population of pacu. Our results provide support for the genetic diversity maintenance and mtDNA traceability of pacu commercial broodstocks. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:56:04Z 2021-06-25T10:56:04Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-4685-GMB-2020-0249 Genetics and Molecular Biology, v. 44, n. 1, 2021. 1678-4685 1415-4757 http://hdl.handle.net/11449/207489 10.1590/1678-4685-GMB-2020-0249 S1415-47572021000100202 2-s2.0-85102931270 S1415-47572021000100202.pdf |
url |
http://dx.doi.org/10.1590/1678-4685-GMB-2020-0249 http://hdl.handle.net/11449/207489 |
identifier_str_mv |
Genetics and Molecular Biology, v. 44, n. 1, 2021. 1678-4685 1415-4757 10.1590/1678-4685-GMB-2020-0249 S1415-47572021000100202 2-s2.0-85102931270 S1415-47572021000100202.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649685852782592 |