Evaluation of N-15 and purine bases as microbial markers to estimate ruminal bacterial nitrogen outflow in dairy cows
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anifeedsci.2019.114297 http://hdl.handle.net/11449/196732 |
Resumo: | The present study aimed to evaluate N-15 and purine bases (PB) as markers for ruminal bacterial nitrogen (N) outflow in dairy cows. Additionally, the effects of calcium salts of fatty acids (CSFA) in diets with different levels of rumen degradable protein (RDP) on PB omasal flow, urinary recovery of purine derivatives (PD), and endogenous excretion of PD were evaluated. Eight ruminal cannulated Holstein cows, with 207 +/- 23.6 (mean +/- SD) days in milk, 618 +/- 39.0 kg of body weight, and 33.4 +/- 2.45 kg/d of milk yield were used in a replicated 4 x 4 Latin square design. Four treatments were arranged in a 2 x 2 factorial design to evaluate the following factors: CSFA addition [-CS: basal diet without CSFA; and + CS: with 33.2 g/kg dry matter (DM) of CSFA]; and the level of RDP (LRDP: low RDP, with 98 g/kg DM of RDP; and HRDP: high RDP, with 110 g/kg DM of RDP). Particle-associated bacteria (PAB) showed lower (P < 0.008) N-15 enrichment and purine N to total bacterial N ratio than liquid associated bacteria (LAB). Increasing RDP concentration reduced (P = 0.007) 15 N enrichment and tended (P < 0.095) to reduce PB concentration in bacterial pellets. Purine bases overestimated (P < 0.002) LAB-NAN and total microbial flow but, regarding PAB-NAN flow, there was a trend of interaction (P = 0.067) between marker and fat supplementation effects. In the absence of fat supplementation, PB underestimated (P < 0.05) the flow of PAB-NAN compared to N-15 and failed to detect the negative effect of fat supplementation evidenced by N-15 labeling. Omasal PB flow tended to increase (P = 0.061) in low protein degradability diets, but PD excretion was constant (322 +/- 5,9 mmol/d, mean +/- SE) regardless of diet and, as a result, urinary recovery of PB decreased (P = 0.007). Daily excretion of creatinine and endogenous PD was also unaffected by the diet and averaged 0.262 mmol/kg BW and 0.640 mmol/kg BW degrees 75 , respectively. In conclusion, the response of PB and its urinary derivatives to variation in diet composition compared to N-15 reveals their weaknesses in the estimation of microbial synthesis. |
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Evaluation of N-15 and purine bases as microbial markers to estimate ruminal bacterial nitrogen outflow in dairy cowsFat supplementationIsotopesMicrobial proteinPurinesUrinary excretionThe present study aimed to evaluate N-15 and purine bases (PB) as markers for ruminal bacterial nitrogen (N) outflow in dairy cows. Additionally, the effects of calcium salts of fatty acids (CSFA) in diets with different levels of rumen degradable protein (RDP) on PB omasal flow, urinary recovery of purine derivatives (PD), and endogenous excretion of PD were evaluated. Eight ruminal cannulated Holstein cows, with 207 +/- 23.6 (mean +/- SD) days in milk, 618 +/- 39.0 kg of body weight, and 33.4 +/- 2.45 kg/d of milk yield were used in a replicated 4 x 4 Latin square design. Four treatments were arranged in a 2 x 2 factorial design to evaluate the following factors: CSFA addition [-CS: basal diet without CSFA; and + CS: with 33.2 g/kg dry matter (DM) of CSFA]; and the level of RDP (LRDP: low RDP, with 98 g/kg DM of RDP; and HRDP: high RDP, with 110 g/kg DM of RDP). Particle-associated bacteria (PAB) showed lower (P < 0.008) N-15 enrichment and purine N to total bacterial N ratio than liquid associated bacteria (LAB). Increasing RDP concentration reduced (P = 0.007) 15 N enrichment and tended (P < 0.095) to reduce PB concentration in bacterial pellets. Purine bases overestimated (P < 0.002) LAB-NAN and total microbial flow but, regarding PAB-NAN flow, there was a trend of interaction (P = 0.067) between marker and fat supplementation effects. In the absence of fat supplementation, PB underestimated (P < 0.05) the flow of PAB-NAN compared to N-15 and failed to detect the negative effect of fat supplementation evidenced by N-15 labeling. Omasal PB flow tended to increase (P = 0.061) in low protein degradability diets, but PD excretion was constant (322 +/- 5,9 mmol/d, mean +/- SE) regardless of diet and, as a result, urinary recovery of PB decreased (P = 0.007). Daily excretion of creatinine and endogenous PD was also unaffected by the diet and averaged 0.262 mmol/kg BW and 0.640 mmol/kg BW degrees 75 , respectively. In conclusion, the response of PB and its urinary derivatives to variation in diet composition compared to N-15 reveals their weaknesses in the estimation of microbial synthesis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Nutr & Anim Prod, BR-13635900 Pirassununga, BrazilUniv Fed Vicosa, Dept Anim Sci, BR-36570000 Vicosa, MG, BrazilSao Paulo State Univ, Inst Biosci, Dept Phys & Biophys, BR-18618689 Botucatu, SP, BrazilSao Paulo State Univ, Inst Biosci, Dept Phys & Biophys, BR-18618689 Botucatu, SP, BrazilFAPESP: 2016/02855-1FAPESP: 2016/11760-4Elsevier B.V.Universidade de São Paulo (USP)Universidade Federal de Viçosa (UFV)Universidade Estadual Paulista (Unesp)Del Valle, T. A.Ghizzi, L. G.Zilio, E. M. C.Marques, J. A.Dias, M. S. S.Silva, T. B. P.Gheller, L. S.Silva, G. G.Sconamiglio, N. T.Nunes, A. T.Renno, L. N.Costa, V. E. [UNESP]Renno, F. P.2020-12-10T19:54:25Z2020-12-10T19:54:25Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.1016/j.anifeedsci.2019.114297Animal Feed Science And Technology. Amsterdam: Elsevier, v. 258, 11 p., 2019.0377-8401http://hdl.handle.net/11449/19673210.1016/j.anifeedsci.2019.114297WOS:000522386100002Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Feed Science And Technologyinfo:eu-repo/semantics/openAccess2021-10-23T09:41:33Zoai:repositorio.unesp.br:11449/196732Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:01:45.731191Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of N-15 and purine bases as microbial markers to estimate ruminal bacterial nitrogen outflow in dairy cows |
title |
Evaluation of N-15 and purine bases as microbial markers to estimate ruminal bacterial nitrogen outflow in dairy cows |
spellingShingle |
Evaluation of N-15 and purine bases as microbial markers to estimate ruminal bacterial nitrogen outflow in dairy cows Del Valle, T. A. Fat supplementation Isotopes Microbial protein Purines Urinary excretion |
title_short |
Evaluation of N-15 and purine bases as microbial markers to estimate ruminal bacterial nitrogen outflow in dairy cows |
title_full |
Evaluation of N-15 and purine bases as microbial markers to estimate ruminal bacterial nitrogen outflow in dairy cows |
title_fullStr |
Evaluation of N-15 and purine bases as microbial markers to estimate ruminal bacterial nitrogen outflow in dairy cows |
title_full_unstemmed |
Evaluation of N-15 and purine bases as microbial markers to estimate ruminal bacterial nitrogen outflow in dairy cows |
title_sort |
Evaluation of N-15 and purine bases as microbial markers to estimate ruminal bacterial nitrogen outflow in dairy cows |
author |
Del Valle, T. A. |
author_facet |
Del Valle, T. A. Ghizzi, L. G. Zilio, E. M. C. Marques, J. A. Dias, M. S. S. Silva, T. B. P. Gheller, L. S. Silva, G. G. Sconamiglio, N. T. Nunes, A. T. Renno, L. N. Costa, V. E. [UNESP] Renno, F. P. |
author_role |
author |
author2 |
Ghizzi, L. G. Zilio, E. M. C. Marques, J. A. Dias, M. S. S. Silva, T. B. P. Gheller, L. S. Silva, G. G. Sconamiglio, N. T. Nunes, A. T. Renno, L. N. Costa, V. E. [UNESP] Renno, F. P. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de Viçosa (UFV) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Del Valle, T. A. Ghizzi, L. G. Zilio, E. M. C. Marques, J. A. Dias, M. S. S. Silva, T. B. P. Gheller, L. S. Silva, G. G. Sconamiglio, N. T. Nunes, A. T. Renno, L. N. Costa, V. E. [UNESP] Renno, F. P. |
dc.subject.por.fl_str_mv |
Fat supplementation Isotopes Microbial protein Purines Urinary excretion |
topic |
Fat supplementation Isotopes Microbial protein Purines Urinary excretion |
description |
The present study aimed to evaluate N-15 and purine bases (PB) as markers for ruminal bacterial nitrogen (N) outflow in dairy cows. Additionally, the effects of calcium salts of fatty acids (CSFA) in diets with different levels of rumen degradable protein (RDP) on PB omasal flow, urinary recovery of purine derivatives (PD), and endogenous excretion of PD were evaluated. Eight ruminal cannulated Holstein cows, with 207 +/- 23.6 (mean +/- SD) days in milk, 618 +/- 39.0 kg of body weight, and 33.4 +/- 2.45 kg/d of milk yield were used in a replicated 4 x 4 Latin square design. Four treatments were arranged in a 2 x 2 factorial design to evaluate the following factors: CSFA addition [-CS: basal diet without CSFA; and + CS: with 33.2 g/kg dry matter (DM) of CSFA]; and the level of RDP (LRDP: low RDP, with 98 g/kg DM of RDP; and HRDP: high RDP, with 110 g/kg DM of RDP). Particle-associated bacteria (PAB) showed lower (P < 0.008) N-15 enrichment and purine N to total bacterial N ratio than liquid associated bacteria (LAB). Increasing RDP concentration reduced (P = 0.007) 15 N enrichment and tended (P < 0.095) to reduce PB concentration in bacterial pellets. Purine bases overestimated (P < 0.002) LAB-NAN and total microbial flow but, regarding PAB-NAN flow, there was a trend of interaction (P = 0.067) between marker and fat supplementation effects. In the absence of fat supplementation, PB underestimated (P < 0.05) the flow of PAB-NAN compared to N-15 and failed to detect the negative effect of fat supplementation evidenced by N-15 labeling. Omasal PB flow tended to increase (P = 0.061) in low protein degradability diets, but PD excretion was constant (322 +/- 5,9 mmol/d, mean +/- SE) regardless of diet and, as a result, urinary recovery of PB decreased (P = 0.007). Daily excretion of creatinine and endogenous PD was also unaffected by the diet and averaged 0.262 mmol/kg BW and 0.640 mmol/kg BW degrees 75 , respectively. In conclusion, the response of PB and its urinary derivatives to variation in diet composition compared to N-15 reveals their weaknesses in the estimation of microbial synthesis. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 2020-12-10T19:54:25Z 2020-12-10T19:54:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anifeedsci.2019.114297 Animal Feed Science And Technology. Amsterdam: Elsevier, v. 258, 11 p., 2019. 0377-8401 http://hdl.handle.net/11449/196732 10.1016/j.anifeedsci.2019.114297 WOS:000522386100002 |
url |
http://dx.doi.org/10.1016/j.anifeedsci.2019.114297 http://hdl.handle.net/11449/196732 |
identifier_str_mv |
Animal Feed Science And Technology. Amsterdam: Elsevier, v. 258, 11 p., 2019. 0377-8401 10.1016/j.anifeedsci.2019.114297 WOS:000522386100002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Feed Science And Technology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128741902123008 |