Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in mice
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.funbio.2018.08.004 http://hdl.handle.net/11449/176860 |
Resumo: | Little is known about the differences in the CD4+ T-cell response induced by Sporothrix schenckii and Sporothrix brasiliensis, the most virulent species that cause sporotrichosis. Here, the helper (Th) and regulatory T cells (Tregs) responses were evaluated comparatively in a murine model of sporotrichosis on days 7, 21 and 35 after subcutaneous infection with either S. schenckii or S. brasiliensis conidia. The fungal load was measured at the site of infection, as well as in the liver and spleen. The Th1/Th17/Tregs responses were analyzed in the spleen, while the level of IL-2, IL-4, IL-6, TNF-alpha, IFN-ɣ, IL-17A and IL-10 cytokines were measured at the local site of infection on 24 h postinfections and in sera on the indicated days. S. brasiliensis caused a longer-lasting infection in the skin and chronic systemic dissemination associated to more severe granulomatous lesions. Similar Th1/Th1-Th17/Tregs responses were induced by both S. brasiliensis and S. schenckii on 7th and 21st d.p.i but on 35 d.p.i a reduction of Th1 and Th1-Th17 cells, associated to higher values of Th17/Tregs cells was observed only in S. brasiliensis-infected mice. In summary, S. brasiliensis caused a more severe disease associated with sustained Th17/Tregs responses than S. schenckii in mice. |
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Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in miceCytokinesSporotrichosisT helper cellsTregsVirulenceLittle is known about the differences in the CD4+ T-cell response induced by Sporothrix schenckii and Sporothrix brasiliensis, the most virulent species that cause sporotrichosis. Here, the helper (Th) and regulatory T cells (Tregs) responses were evaluated comparatively in a murine model of sporotrichosis on days 7, 21 and 35 after subcutaneous infection with either S. schenckii or S. brasiliensis conidia. The fungal load was measured at the site of infection, as well as in the liver and spleen. The Th1/Th17/Tregs responses were analyzed in the spleen, while the level of IL-2, IL-4, IL-6, TNF-alpha, IFN-ɣ, IL-17A and IL-10 cytokines were measured at the local site of infection on 24 h postinfections and in sera on the indicated days. S. brasiliensis caused a longer-lasting infection in the skin and chronic systemic dissemination associated to more severe granulomatous lesions. Similar Th1/Th1-Th17/Tregs responses were induced by both S. brasiliensis and S. schenckii on 7th and 21st d.p.i but on 35 d.p.i a reduction of Th1 and Th1-Th17 cells, associated to higher values of Th17/Tregs cells was observed only in S. brasiliensis-infected mice. In summary, S. brasiliensis caused a more severe disease associated with sustained Th17/Tregs responses than S. schenckii in mice.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical AnalysisSão Paulo State University (UNESP) School of Dentistry Department of Physiology & PathologyLaboratório de Imunología Clínica Dpto Analises Clinicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (UNESP) Rod, Araraquara-Jaú – Km 1, Campus VilleSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Clinical AnalysisSão Paulo State University (UNESP) School of Dentistry Department of Physiology & PathologyLaboratório de Imunología Clínica Dpto Analises Clinicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (UNESP) Rod, Araraquara-Jaú – Km 1, Campus VilleUniversidade Estadual Paulista (Unesp)Batista-Duharte, Alexander [UNESP]Téllez-Martínez, Damiana [UNESP]Roberto de Andrade, Cleverton [UNESP]Portuondo, Deivys Leandro [UNESP]Jellmayer, Juliana Aparecida [UNESP]Polesi, Marisa Campos [UNESP]Carlos, Iracilda Zeppone [UNESP]2018-12-11T17:22:49Z2018-12-11T17:22:49Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1016/j.funbio.2018.08.004Fungal Biology.1878-6146http://hdl.handle.net/11449/17686010.1016/j.funbio.2018.08.0042-s2.0-850536155282-s2.0-85053615528.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFungal Biology1,134info:eu-repo/semantics/openAccess2024-06-21T15:18:45Zoai:repositorio.unesp.br:11449/176860Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:40:11.248426Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in mice |
title |
Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in mice |
spellingShingle |
Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in mice Batista-Duharte, Alexander [UNESP] Cytokines Sporotrichosis T helper cells Tregs Virulence |
title_short |
Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in mice |
title_full |
Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in mice |
title_fullStr |
Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in mice |
title_full_unstemmed |
Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in mice |
title_sort |
Sporothrix brasiliensis induces a more severe disease associated with sustained Th17 and regulatory T cells responses than Sporothrix schenckii sensu stricto in mice |
author |
Batista-Duharte, Alexander [UNESP] |
author_facet |
Batista-Duharte, Alexander [UNESP] Téllez-Martínez, Damiana [UNESP] Roberto de Andrade, Cleverton [UNESP] Portuondo, Deivys Leandro [UNESP] Jellmayer, Juliana Aparecida [UNESP] Polesi, Marisa Campos [UNESP] Carlos, Iracilda Zeppone [UNESP] |
author_role |
author |
author2 |
Téllez-Martínez, Damiana [UNESP] Roberto de Andrade, Cleverton [UNESP] Portuondo, Deivys Leandro [UNESP] Jellmayer, Juliana Aparecida [UNESP] Polesi, Marisa Campos [UNESP] Carlos, Iracilda Zeppone [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Batista-Duharte, Alexander [UNESP] Téllez-Martínez, Damiana [UNESP] Roberto de Andrade, Cleverton [UNESP] Portuondo, Deivys Leandro [UNESP] Jellmayer, Juliana Aparecida [UNESP] Polesi, Marisa Campos [UNESP] Carlos, Iracilda Zeppone [UNESP] |
dc.subject.por.fl_str_mv |
Cytokines Sporotrichosis T helper cells Tregs Virulence |
topic |
Cytokines Sporotrichosis T helper cells Tregs Virulence |
description |
Little is known about the differences in the CD4+ T-cell response induced by Sporothrix schenckii and Sporothrix brasiliensis, the most virulent species that cause sporotrichosis. Here, the helper (Th) and regulatory T cells (Tregs) responses were evaluated comparatively in a murine model of sporotrichosis on days 7, 21 and 35 after subcutaneous infection with either S. schenckii or S. brasiliensis conidia. The fungal load was measured at the site of infection, as well as in the liver and spleen. The Th1/Th17/Tregs responses were analyzed in the spleen, while the level of IL-2, IL-4, IL-6, TNF-alpha, IFN-ɣ, IL-17A and IL-10 cytokines were measured at the local site of infection on 24 h postinfections and in sera on the indicated days. S. brasiliensis caused a longer-lasting infection in the skin and chronic systemic dissemination associated to more severe granulomatous lesions. Similar Th1/Th1-Th17/Tregs responses were induced by both S. brasiliensis and S. schenckii on 7th and 21st d.p.i but on 35 d.p.i a reduction of Th1 and Th1-Th17 cells, associated to higher values of Th17/Tregs cells was observed only in S. brasiliensis-infected mice. In summary, S. brasiliensis caused a more severe disease associated with sustained Th17/Tregs responses than S. schenckii in mice. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:22:49Z 2018-12-11T17:22:49Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.funbio.2018.08.004 Fungal Biology. 1878-6146 http://hdl.handle.net/11449/176860 10.1016/j.funbio.2018.08.004 2-s2.0-85053615528 2-s2.0-85053615528.pdf |
url |
http://dx.doi.org/10.1016/j.funbio.2018.08.004 http://hdl.handle.net/11449/176860 |
identifier_str_mv |
Fungal Biology. 1878-6146 10.1016/j.funbio.2018.08.004 2-s2.0-85053615528 2-s2.0-85053615528.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fungal Biology 1,134 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128685526482944 |