Long-Term Effect of Daily Chemical Disinfection on Surface Topography and Candida Albicans Biofilm Formation on Denture Base and Reline Acrylic Resins
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3290/j.ohpd.a45521 http://hdl.handle.net/11449/210694 |
Resumo: | Purpose: This study investigated the effect of long-term daily chemical disinfection on the topographic and Candida albicans biofilm formation on a denture base resin and a reline acrylic resin. Material and Methods: Circular samples (14 x 1.2 mm) were fabricated from a denture base (Vipi Wave) and reline acrylic resins (Tokuyama Rebase Fast II). Samples were kept in 50 ml of distilled water (48 h at 37 degrees C). Subsequently, the samples were immersed in five different solutions: 0.5% sodium hypochlorite; 3.8% sodium perborate; 2% chlorhexidine gluconate; apple vinegar containing 4% maleic acid; and distilled water (control group). The specimen was immersed in the solutions for 8 h daily and transferred to distilled water at 37 degrees C for more 16 h. The surface topographic and Candida albicans (ATCC 90028) biofilm formation were evaluated at baseline (before chemical disinfection) and after 1, 3 and 6 months of immersion. The surface topographic was evaluated by arithmetical roughness average (Ra) and scanning electron microscope (SEM), while the biofilm formation was evaluated by colony-forming units (CFU/ml) method and Alamar Blue assay (cell metabolism). The results were evaluated by three-way analysis of variance (ANOVAs) and post-hoc tests (alpha = 0.05). Results: The results showed statistically significant effects from the type of acrylic resin (p = 0.029) and time (p<0.001) on the roughness of the specimen. In general, the reline resin had higher roughness than the denture base resin. In addition, the roughness of the samples after 1, 3 and 6 months of immersion in the cleaning solutions was higher than at baseline. In relation to the microbiological assays, there were no statistically significant differences (p > 0.055) in the CFU/ml values of the biofilms among the different resins, periods of time and cleaning solutions. Considering the metabolism of the cells within the biofilms, the results showed that, at baseline, it was statistically significantly higher (p<0.05) than after 1, 3 and 6 months of storage. The SEM images showed that all disinfectant solutions provided surface changes of both acrylic resins (base and reline) after 1, 3 and 6 months of immersion. Conclusions: The roughness of both acrylic resins was affected by the disinfection in all cleaning agents, increasing over time, and this effect was more evident in the reline acrylic resin group. This surface change was also observed in the SEM images. While the number of cells within the biofilms was not affected by immersion in the cleaning agents, their metabolism was lower after 1, 3 and 6 months of immersion. |
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Repositório Institucional da UNESP |
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Long-Term Effect of Daily Chemical Disinfection on Surface Topography and Candida Albicans Biofilm Formation on Denture Base and Reline Acrylic Resinsbiofilmacrylic base resinhard relinesroughnessdenture cleansersPurpose: This study investigated the effect of long-term daily chemical disinfection on the topographic and Candida albicans biofilm formation on a denture base resin and a reline acrylic resin. Material and Methods: Circular samples (14 x 1.2 mm) were fabricated from a denture base (Vipi Wave) and reline acrylic resins (Tokuyama Rebase Fast II). Samples were kept in 50 ml of distilled water (48 h at 37 degrees C). Subsequently, the samples were immersed in five different solutions: 0.5% sodium hypochlorite; 3.8% sodium perborate; 2% chlorhexidine gluconate; apple vinegar containing 4% maleic acid; and distilled water (control group). The specimen was immersed in the solutions for 8 h daily and transferred to distilled water at 37 degrees C for more 16 h. The surface topographic and Candida albicans (ATCC 90028) biofilm formation were evaluated at baseline (before chemical disinfection) and after 1, 3 and 6 months of immersion. The surface topographic was evaluated by arithmetical roughness average (Ra) and scanning electron microscope (SEM), while the biofilm formation was evaluated by colony-forming units (CFU/ml) method and Alamar Blue assay (cell metabolism). The results were evaluated by three-way analysis of variance (ANOVAs) and post-hoc tests (alpha = 0.05). Results: The results showed statistically significant effects from the type of acrylic resin (p = 0.029) and time (p<0.001) on the roughness of the specimen. In general, the reline resin had higher roughness than the denture base resin. In addition, the roughness of the samples after 1, 3 and 6 months of immersion in the cleaning solutions was higher than at baseline. In relation to the microbiological assays, there were no statistically significant differences (p > 0.055) in the CFU/ml values of the biofilms among the different resins, periods of time and cleaning solutions. Considering the metabolism of the cells within the biofilms, the results showed that, at baseline, it was statistically significantly higher (p<0.05) than after 1, 3 and 6 months of storage. The SEM images showed that all disinfectant solutions provided surface changes of both acrylic resins (base and reline) after 1, 3 and 6 months of immersion. Conclusions: The roughness of both acrylic resins was affected by the disinfection in all cleaning agents, increasing over time, and this effect was more evident in the reline acrylic resin group. This surface change was also observed in the SEM images. While the number of cells within the biofilms was not affected by immersion in the cleaning agents, their metabolism was lower after 1, 3 and 6 months of immersion.Sao Paulo State Univ UNESP, Araraquara Dent Sch, Campus Araraquara, Araraquara, SP, BrazilSao Paulo State Univ UNESP, Araraquara Dent Sch, Campus Araraquara, Araraquara, SP, BrazilQuintessence Publishing Co IncUniversidade Estadual Paulista (Unesp)Amaya Arbelaez, Maria Isabel [UNESP]Vergani, Carlos Eduardo [UNESP]Barbugli, Paula Aboud [UNESP]Pavarina, Ana Claudia [UNESP]Sanita, Paula Volpato [UNESP]Jorge, Janaina Habib [UNESP]2021-06-26T02:54:12Z2021-06-26T02:54:12Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article999-1010http://dx.doi.org/10.3290/j.ohpd.a45521Oral Health & Preventive Dentistry. Hanover Park: Quintessence Publishing Co Inc, v. 18, n. 1, p. 999-1010, 2020.1602-1622http://hdl.handle.net/11449/21069410.3290/j.ohpd.a45521WOS:000619799200020Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOral Health & Preventive Dentistryinfo:eu-repo/semantics/openAccess2021-10-23T22:13:49Zoai:repositorio.unesp.br:11449/210694Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:50:40.467964Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Long-Term Effect of Daily Chemical Disinfection on Surface Topography and Candida Albicans Biofilm Formation on Denture Base and Reline Acrylic Resins |
title |
Long-Term Effect of Daily Chemical Disinfection on Surface Topography and Candida Albicans Biofilm Formation on Denture Base and Reline Acrylic Resins |
spellingShingle |
Long-Term Effect of Daily Chemical Disinfection on Surface Topography and Candida Albicans Biofilm Formation on Denture Base and Reline Acrylic Resins Amaya Arbelaez, Maria Isabel [UNESP] biofilm acrylic base resin hard relines roughness denture cleansers |
title_short |
Long-Term Effect of Daily Chemical Disinfection on Surface Topography and Candida Albicans Biofilm Formation on Denture Base and Reline Acrylic Resins |
title_full |
Long-Term Effect of Daily Chemical Disinfection on Surface Topography and Candida Albicans Biofilm Formation on Denture Base and Reline Acrylic Resins |
title_fullStr |
Long-Term Effect of Daily Chemical Disinfection on Surface Topography and Candida Albicans Biofilm Formation on Denture Base and Reline Acrylic Resins |
title_full_unstemmed |
Long-Term Effect of Daily Chemical Disinfection on Surface Topography and Candida Albicans Biofilm Formation on Denture Base and Reline Acrylic Resins |
title_sort |
Long-Term Effect of Daily Chemical Disinfection on Surface Topography and Candida Albicans Biofilm Formation on Denture Base and Reline Acrylic Resins |
author |
Amaya Arbelaez, Maria Isabel [UNESP] |
author_facet |
Amaya Arbelaez, Maria Isabel [UNESP] Vergani, Carlos Eduardo [UNESP] Barbugli, Paula Aboud [UNESP] Pavarina, Ana Claudia [UNESP] Sanita, Paula Volpato [UNESP] Jorge, Janaina Habib [UNESP] |
author_role |
author |
author2 |
Vergani, Carlos Eduardo [UNESP] Barbugli, Paula Aboud [UNESP] Pavarina, Ana Claudia [UNESP] Sanita, Paula Volpato [UNESP] Jorge, Janaina Habib [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Amaya Arbelaez, Maria Isabel [UNESP] Vergani, Carlos Eduardo [UNESP] Barbugli, Paula Aboud [UNESP] Pavarina, Ana Claudia [UNESP] Sanita, Paula Volpato [UNESP] Jorge, Janaina Habib [UNESP] |
dc.subject.por.fl_str_mv |
biofilm acrylic base resin hard relines roughness denture cleansers |
topic |
biofilm acrylic base resin hard relines roughness denture cleansers |
description |
Purpose: This study investigated the effect of long-term daily chemical disinfection on the topographic and Candida albicans biofilm formation on a denture base resin and a reline acrylic resin. Material and Methods: Circular samples (14 x 1.2 mm) were fabricated from a denture base (Vipi Wave) and reline acrylic resins (Tokuyama Rebase Fast II). Samples were kept in 50 ml of distilled water (48 h at 37 degrees C). Subsequently, the samples were immersed in five different solutions: 0.5% sodium hypochlorite; 3.8% sodium perborate; 2% chlorhexidine gluconate; apple vinegar containing 4% maleic acid; and distilled water (control group). The specimen was immersed in the solutions for 8 h daily and transferred to distilled water at 37 degrees C for more 16 h. The surface topographic and Candida albicans (ATCC 90028) biofilm formation were evaluated at baseline (before chemical disinfection) and after 1, 3 and 6 months of immersion. The surface topographic was evaluated by arithmetical roughness average (Ra) and scanning electron microscope (SEM), while the biofilm formation was evaluated by colony-forming units (CFU/ml) method and Alamar Blue assay (cell metabolism). The results were evaluated by three-way analysis of variance (ANOVAs) and post-hoc tests (alpha = 0.05). Results: The results showed statistically significant effects from the type of acrylic resin (p = 0.029) and time (p<0.001) on the roughness of the specimen. In general, the reline resin had higher roughness than the denture base resin. In addition, the roughness of the samples after 1, 3 and 6 months of immersion in the cleaning solutions was higher than at baseline. In relation to the microbiological assays, there were no statistically significant differences (p > 0.055) in the CFU/ml values of the biofilms among the different resins, periods of time and cleaning solutions. Considering the metabolism of the cells within the biofilms, the results showed that, at baseline, it was statistically significantly higher (p<0.05) than after 1, 3 and 6 months of storage. The SEM images showed that all disinfectant solutions provided surface changes of both acrylic resins (base and reline) after 1, 3 and 6 months of immersion. Conclusions: The roughness of both acrylic resins was affected by the disinfection in all cleaning agents, increasing over time, and this effect was more evident in the reline acrylic resin group. This surface change was also observed in the SEM images. While the number of cells within the biofilms was not affected by immersion in the cleaning agents, their metabolism was lower after 1, 3 and 6 months of immersion. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 2021-06-26T02:54:12Z 2021-06-26T02:54:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3290/j.ohpd.a45521 Oral Health & Preventive Dentistry. Hanover Park: Quintessence Publishing Co Inc, v. 18, n. 1, p. 999-1010, 2020. 1602-1622 http://hdl.handle.net/11449/210694 10.3290/j.ohpd.a45521 WOS:000619799200020 |
url |
http://dx.doi.org/10.3290/j.ohpd.a45521 http://hdl.handle.net/11449/210694 |
identifier_str_mv |
Oral Health & Preventive Dentistry. Hanover Park: Quintessence Publishing Co Inc, v. 18, n. 1, p. 999-1010, 2020. 1602-1622 10.3290/j.ohpd.a45521 WOS:000619799200020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oral Health & Preventive Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
999-1010 |
dc.publisher.none.fl_str_mv |
Quintessence Publishing Co Inc |
publisher.none.fl_str_mv |
Quintessence Publishing Co Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129365351858176 |