HMGB1 and inflammatory cytokines in experimental acute lung injury induced in rabbits

Detalhes bibliográficos
Autor(a) principal: Kurokawa, C. S. [UNESP]
Data de Publicação: 2020
Outros Autores: Júnior, J.P. Araújo [UNESP], Pires, R. B. [UNESP], Carpi, M. F. [UNESP], Moraes, M. A. [UNESP], Medeiros, L. T.L. [UNESP], Fioretto, J. R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1678-4162-11499
http://hdl.handle.net/11449/199383
Resumo: The aim of this work was to measure HMGB1, TNF-alpha, and IL-8 in bronchoalveolar lavage (BAL), serum and TLR2 and TLR4mRNA expression in lung tissue of rabbits with two grades of acute lung injury (ALI). The animals were randomly assigned to groups with severe (S) and mild/moderate (MM) ALI, induced with warm saline, and a control group. HMGB1, TNF-alpha, IL-8, TLR2mRNA and TLR4mRNA were measured after ALI induction. The results showed increased levels of IL-8, TNF-alpha, HMGB1 and TLR4mRNA in the ALI groups. HMGB1, IL-8 and TNF-alpha concentrations in BAL were higher in S compared MM. Increased TLR4mRNA was observed in S and MM versus control. The results suggest an early participation of HMGB1 in ALI together with IL-8 and TNF-alpha and association with severity. TLR4 has early expression and role in ALI pathophysiology but is not associated with severity.
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spelling HMGB1 and inflammatory cytokines in experimental acute lung injury induced in rabbitsAcute lung lesion (ALI)CytokinesHMGB-1Lung lesionRabbitThe aim of this work was to measure HMGB1, TNF-alpha, and IL-8 in bronchoalveolar lavage (BAL), serum and TLR2 and TLR4mRNA expression in lung tissue of rabbits with two grades of acute lung injury (ALI). The animals were randomly assigned to groups with severe (S) and mild/moderate (MM) ALI, induced with warm saline, and a control group. HMGB1, TNF-alpha, IL-8, TLR2mRNA and TLR4mRNA were measured after ALI induction. The results showed increased levels of IL-8, TNF-alpha, HMGB1 and TLR4mRNA in the ALI groups. HMGB1, IL-8 and TNF-alpha concentrations in BAL were higher in S compared MM. Increased TLR4mRNA was observed in S and MM versus control. The results suggest an early participation of HMGB1 in ALI together with IL-8 and TNF-alpha and association with severity. TLR4 has early expression and role in ALI pathophysiology but is not associated with severity.Unesp Faculdade de MedicinaUnesp Instituto de BiociênciasUnesp Faculdade de MedicinaUnesp Instituto de BiociênciasUniversidade Estadual Paulista (Unesp)Kurokawa, C. S. [UNESP]Júnior, J.P. Araújo [UNESP]Pires, R. B. [UNESP]Carpi, M. F. [UNESP]Moraes, M. A. [UNESP]Medeiros, L. T.L. [UNESP]Fioretto, J. R. [UNESP]2020-12-12T01:38:15Z2020-12-12T01:38:15Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1329-1338application/pdfhttp://dx.doi.org/10.1590/1678-4162-11499Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 72, n. 4, p. 1329-1338, 2020.1678-41620102-0935http://hdl.handle.net/11449/19938310.1590/1678-4162-11499S0102-093520200004013292-s2.0-85090759700S0102-09352020000401329.pdf85104232695404650000-0003-1380-7527Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro de Medicina Veterinaria e Zootecniainfo:eu-repo/semantics/openAccess2023-10-20T06:11:10Zoai:repositorio.unesp.br:11449/199383Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:29:44.875838Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv HMGB1 and inflammatory cytokines in experimental acute lung injury induced in rabbits
title HMGB1 and inflammatory cytokines in experimental acute lung injury induced in rabbits
spellingShingle HMGB1 and inflammatory cytokines in experimental acute lung injury induced in rabbits
Kurokawa, C. S. [UNESP]
Acute lung lesion (ALI)
Cytokines
HMGB-1
Lung lesion
Rabbit
title_short HMGB1 and inflammatory cytokines in experimental acute lung injury induced in rabbits
title_full HMGB1 and inflammatory cytokines in experimental acute lung injury induced in rabbits
title_fullStr HMGB1 and inflammatory cytokines in experimental acute lung injury induced in rabbits
title_full_unstemmed HMGB1 and inflammatory cytokines in experimental acute lung injury induced in rabbits
title_sort HMGB1 and inflammatory cytokines in experimental acute lung injury induced in rabbits
author Kurokawa, C. S. [UNESP]
author_facet Kurokawa, C. S. [UNESP]
Júnior, J.P. Araújo [UNESP]
Pires, R. B. [UNESP]
Carpi, M. F. [UNESP]
Moraes, M. A. [UNESP]
Medeiros, L. T.L. [UNESP]
Fioretto, J. R. [UNESP]
author_role author
author2 Júnior, J.P. Araújo [UNESP]
Pires, R. B. [UNESP]
Carpi, M. F. [UNESP]
Moraes, M. A. [UNESP]
Medeiros, L. T.L. [UNESP]
Fioretto, J. R. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Kurokawa, C. S. [UNESP]
Júnior, J.P. Araújo [UNESP]
Pires, R. B. [UNESP]
Carpi, M. F. [UNESP]
Moraes, M. A. [UNESP]
Medeiros, L. T.L. [UNESP]
Fioretto, J. R. [UNESP]
dc.subject.por.fl_str_mv Acute lung lesion (ALI)
Cytokines
HMGB-1
Lung lesion
Rabbit
topic Acute lung lesion (ALI)
Cytokines
HMGB-1
Lung lesion
Rabbit
description The aim of this work was to measure HMGB1, TNF-alpha, and IL-8 in bronchoalveolar lavage (BAL), serum and TLR2 and TLR4mRNA expression in lung tissue of rabbits with two grades of acute lung injury (ALI). The animals were randomly assigned to groups with severe (S) and mild/moderate (MM) ALI, induced with warm saline, and a control group. HMGB1, TNF-alpha, IL-8, TLR2mRNA and TLR4mRNA were measured after ALI induction. The results showed increased levels of IL-8, TNF-alpha, HMGB1 and TLR4mRNA in the ALI groups. HMGB1, IL-8 and TNF-alpha concentrations in BAL were higher in S compared MM. Increased TLR4mRNA was observed in S and MM versus control. The results suggest an early participation of HMGB1 in ALI together with IL-8 and TNF-alpha and association with severity. TLR4 has early expression and role in ALI pathophysiology but is not associated with severity.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:38:15Z
2020-12-12T01:38:15Z
2020-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1678-4162-11499
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 72, n. 4, p. 1329-1338, 2020.
1678-4162
0102-0935
http://hdl.handle.net/11449/199383
10.1590/1678-4162-11499
S0102-09352020000401329
2-s2.0-85090759700
S0102-09352020000401329.pdf
8510423269540465
0000-0003-1380-7527
url http://dx.doi.org/10.1590/1678-4162-11499
http://hdl.handle.net/11449/199383
identifier_str_mv Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 72, n. 4, p. 1329-1338, 2020.
1678-4162
0102-0935
10.1590/1678-4162-11499
S0102-09352020000401329
2-s2.0-85090759700
S0102-09352020000401329.pdf
8510423269540465
0000-0003-1380-7527
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinaria e Zootecnia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1329-1338
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128520354791424

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