Detection of differentially methylated regions of irradiated fig tree selections
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-992X-2016-0195 http://hdl.handle.net/11449/162806 |
Resumo: | Fig tree (Ficus carica L.) breeding programs using conventional methods, such as directed crosses, to obtain new cultivars, are unworkable in many countries, including Brazil. Consequently, genetic breeding through mutagenesis has emerged as an important line of research that can improve this crop, and be a significant source of information about this species and assist in the implementation of propagation projects and appropriate management. The aim of this study was to verify the existence of epigenetic variability attributable to DNA methylation in irradiated fig selections when compared both to each other and to the main commercial cultivar, Roxo-de-Valinhos, which had previously used methylation-sensitive amplified polymorphism (MSAP) and DNA sequencing to detect the position of polymorphic regions, analyzable by bioinformatic tools. The sequencing of DNA, isolated from the differentially methylated sites, makes it possible to observe different patterns of methylation by sequencing the treated DNA with sodium bisulfite in the coding regions of regulatory genes active in the development, and fruit ripening stages. Furthermore, they have been found in the mitochondrial DNA of treatments which regulate the supply of energy in Adenosine triphosphate (ATP) form in plants. Closely related to their development, they justify the different phenotypes found in both fruit and plant growth that have suffered stress due to exposure to gamma radiation. Thus, future studies on gene expression in treatments have emerged as an extremely important strategy for understanding these complex regulatory systems, which may lead to the identification of genes of agricultural interest for the fig tree crop, and allow for manipulation and subsequent propagation of improved crops for commercial purposes. |
id |
UNSP_82da8b23bb6d9455c9a8fbe36e8d6da1 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/162806 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Detection of differentially methylated regions of irradiated fig tree selectionsFicus caricaDMRsmethylationplant breedingepigeneticsFig tree (Ficus carica L.) breeding programs using conventional methods, such as directed crosses, to obtain new cultivars, are unworkable in many countries, including Brazil. Consequently, genetic breeding through mutagenesis has emerged as an important line of research that can improve this crop, and be a significant source of information about this species and assist in the implementation of propagation projects and appropriate management. The aim of this study was to verify the existence of epigenetic variability attributable to DNA methylation in irradiated fig selections when compared both to each other and to the main commercial cultivar, Roxo-de-Valinhos, which had previously used methylation-sensitive amplified polymorphism (MSAP) and DNA sequencing to detect the position of polymorphic regions, analyzable by bioinformatic tools. The sequencing of DNA, isolated from the differentially methylated sites, makes it possible to observe different patterns of methylation by sequencing the treated DNA with sodium bisulfite in the coding regions of regulatory genes active in the development, and fruit ripening stages. Furthermore, they have been found in the mitochondrial DNA of treatments which regulate the supply of energy in Adenosine triphosphate (ATP) form in plants. Closely related to their development, they justify the different phenotypes found in both fruit and plant growth that have suffered stress due to exposure to gamma radiation. Thus, future studies on gene expression in treatments have emerged as an extremely important strategy for understanding these complex regulatory systems, which may lead to the identification of genes of agricultural interest for the fig tree crop, and allow for manipulation and subsequent propagation of improved crops for commercial purposes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Post Graduate Program in Genetics of University of Sao Paulo Medical SchoolSao Paulo State Univ, Fac Agr & Technol Sci, Rod Comandante Joao Ribeiro de Barros Km 651, BR-17900000 Dracena, SP, BrazilUniv Sao Paulo, FMRP, Dept Genet, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Zootechny, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilSao Paulo State Univ, Fac Agr & Technol Sci, Rod Comandante Joao Ribeiro de Barros Km 651, BR-17900000 Dracena, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Zootechny, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilUniv Sao PaoloUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Fontanetti Rodrigues, Maria Gabriela [UNESP]Soares, Murilo RacyJovino Gimenez, Daniele Fernanda [UNESP]Simielli Fonseca, Larissa Fernanda [UNESP]Torrieri, EricoRamos, Ester SilveiraGiuliatti, Silvana2018-11-26T17:31:28Z2018-11-26T17:31:28Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article285-293application/pdfhttp://dx.doi.org/10.1590/1678-992X-2016-0195Scientia Agricola. Cerquera Cesar: Univ Sao Paolo, v. 74, n. 4, p. 285-293, 2017.1678-992Xhttp://hdl.handle.net/11449/16280610.1590/1678-992X-2016-0195S0103-90162017000400285WOS:000401542200004S0103-90162017000400285.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientia Agricolainfo:eu-repo/semantics/openAccess2024-06-07T18:44:29Zoai:repositorio.unesp.br:11449/162806Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:48:26.782309Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detection of differentially methylated regions of irradiated fig tree selections |
title |
Detection of differentially methylated regions of irradiated fig tree selections |
spellingShingle |
Detection of differentially methylated regions of irradiated fig tree selections Fontanetti Rodrigues, Maria Gabriela [UNESP] Ficus carica DMRs methylation plant breeding epigenetics |
title_short |
Detection of differentially methylated regions of irradiated fig tree selections |
title_full |
Detection of differentially methylated regions of irradiated fig tree selections |
title_fullStr |
Detection of differentially methylated regions of irradiated fig tree selections |
title_full_unstemmed |
Detection of differentially methylated regions of irradiated fig tree selections |
title_sort |
Detection of differentially methylated regions of irradiated fig tree selections |
author |
Fontanetti Rodrigues, Maria Gabriela [UNESP] |
author_facet |
Fontanetti Rodrigues, Maria Gabriela [UNESP] Soares, Murilo Racy Jovino Gimenez, Daniele Fernanda [UNESP] Simielli Fonseca, Larissa Fernanda [UNESP] Torrieri, Erico Ramos, Ester Silveira Giuliatti, Silvana |
author_role |
author |
author2 |
Soares, Murilo Racy Jovino Gimenez, Daniele Fernanda [UNESP] Simielli Fonseca, Larissa Fernanda [UNESP] Torrieri, Erico Ramos, Ester Silveira Giuliatti, Silvana |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Fontanetti Rodrigues, Maria Gabriela [UNESP] Soares, Murilo Racy Jovino Gimenez, Daniele Fernanda [UNESP] Simielli Fonseca, Larissa Fernanda [UNESP] Torrieri, Erico Ramos, Ester Silveira Giuliatti, Silvana |
dc.subject.por.fl_str_mv |
Ficus carica DMRs methylation plant breeding epigenetics |
topic |
Ficus carica DMRs methylation plant breeding epigenetics |
description |
Fig tree (Ficus carica L.) breeding programs using conventional methods, such as directed crosses, to obtain new cultivars, are unworkable in many countries, including Brazil. Consequently, genetic breeding through mutagenesis has emerged as an important line of research that can improve this crop, and be a significant source of information about this species and assist in the implementation of propagation projects and appropriate management. The aim of this study was to verify the existence of epigenetic variability attributable to DNA methylation in irradiated fig selections when compared both to each other and to the main commercial cultivar, Roxo-de-Valinhos, which had previously used methylation-sensitive amplified polymorphism (MSAP) and DNA sequencing to detect the position of polymorphic regions, analyzable by bioinformatic tools. The sequencing of DNA, isolated from the differentially methylated sites, makes it possible to observe different patterns of methylation by sequencing the treated DNA with sodium bisulfite in the coding regions of regulatory genes active in the development, and fruit ripening stages. Furthermore, they have been found in the mitochondrial DNA of treatments which regulate the supply of energy in Adenosine triphosphate (ATP) form in plants. Closely related to their development, they justify the different phenotypes found in both fruit and plant growth that have suffered stress due to exposure to gamma radiation. Thus, future studies on gene expression in treatments have emerged as an extremely important strategy for understanding these complex regulatory systems, which may lead to the identification of genes of agricultural interest for the fig tree crop, and allow for manipulation and subsequent propagation of improved crops for commercial purposes. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-01 2018-11-26T17:31:28Z 2018-11-26T17:31:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-992X-2016-0195 Scientia Agricola. Cerquera Cesar: Univ Sao Paolo, v. 74, n. 4, p. 285-293, 2017. 1678-992X http://hdl.handle.net/11449/162806 10.1590/1678-992X-2016-0195 S0103-90162017000400285 WOS:000401542200004 S0103-90162017000400285.pdf |
url |
http://dx.doi.org/10.1590/1678-992X-2016-0195 http://hdl.handle.net/11449/162806 |
identifier_str_mv |
Scientia Agricola. Cerquera Cesar: Univ Sao Paolo, v. 74, n. 4, p. 285-293, 2017. 1678-992X 10.1590/1678-992X-2016-0195 S0103-90162017000400285 WOS:000401542200004 S0103-90162017000400285.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientia Agricola |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
285-293 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Sao Paolo |
publisher.none.fl_str_mv |
Univ Sao Paolo |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129463791124480 |