Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms23020971 http://hdl.handle.net/11449/223253 |
Resumo: | This study aimed at engineering cytocompatible and injectable antibiotic-laden fibrous microparticles gelatin methacryloyl (GelMA) hydrogels for endodontic infection ablation. Clindamycin (CLIN) or metronidazole (MET) was added to a polymer solution and electrospun into fibrous mats, which were processed via cryomilling to obtain CLIN-or MET-laden fibrous microparticles. Then, GelMA was modified with CLIN-or MET-laden microparticles or by using equal amounts of each set of fibrous microparticles. Morphological characterization of electrospun fibers and cryomilled particles was performed via scanning electron microscopy (SEM). The experimental hydrogels were further examined for swelling, degradation, and toxicity to dental stem cells, as well as antimicrobial action against endodontic pathogens (agar diffusion) and biofilm inhibition, evaluated both quantitatively (CFU/mL) and qualitatively via confocal laser scanning microscopy (CLSM) and SEM. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). The modification of GelMA with antibiotic-laden fibrous microparticles increased the hydrogel swelling ratio and degradation rate. Cell viability was slightly reduced, although without any significant toxicity (cell viability > 50%). All hydrogels containing antibiotic-laden fibrous microparticles displayed antibiofilm effects, with the dentin substrate showing nearly complete elimination of viable bacteria. Altogether, our findings suggest that the engineered injectable antibiotic-laden fibrous microparticles hydrogels hold clinical prospects for endodontic infection ablation. |
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Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection AblationAntibioticsBiodegradationCryomillingDentistryElectrospinningEndodonticsFibrous particlesRegenerationThis study aimed at engineering cytocompatible and injectable antibiotic-laden fibrous microparticles gelatin methacryloyl (GelMA) hydrogels for endodontic infection ablation. Clindamycin (CLIN) or metronidazole (MET) was added to a polymer solution and electrospun into fibrous mats, which were processed via cryomilling to obtain CLIN-or MET-laden fibrous microparticles. Then, GelMA was modified with CLIN-or MET-laden microparticles or by using equal amounts of each set of fibrous microparticles. Morphological characterization of electrospun fibers and cryomilled particles was performed via scanning electron microscopy (SEM). The experimental hydrogels were further examined for swelling, degradation, and toxicity to dental stem cells, as well as antimicrobial action against endodontic pathogens (agar diffusion) and biofilm inhibition, evaluated both quantitatively (CFU/mL) and qualitatively via confocal laser scanning microscopy (CLSM) and SEM. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). The modification of GelMA with antibiotic-laden fibrous microparticles increased the hydrogel swelling ratio and degradation rate. Cell viability was slightly reduced, although without any significant toxicity (cell viability > 50%). All hydrogels containing antibiotic-laden fibrous microparticles displayed antibiofilm effects, with the dentin substrate showing nearly complete elimination of viable bacteria. Altogether, our findings suggest that the engineered injectable antibiotic-laden fibrous microparticles hydrogels hold clinical prospects for endodontic infection ablation.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Institute of Dental and Craniofacial ResearchNational Institutes of HealthDepartment of Cariology Restorative Sciences and Endodontics School of Dentistry University of MichiganDepartment of Restorative Dentistry School of Dentistry Federal University of Pelotas, Rio Grande do SulDepartment of Conservative Dentistry School of Dentistry Federal University of Rio Grande do Sul, Rio Grande do SulDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University, São PauloDepartment of Biologic and Materials Sciences & Prosthodontics University of Michigan School of DentistryDepartment of Pharmaceutical Sciences and the Biointerfaces Institute University of MichiganDepartment of Biomedical Engineering College of Engineering University of MichiganDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University, São PauloFAPESP: 2016/15674-5FAPESP: 2018/14257-7National Institute of Dental and Craniofacial Research: K08DE023552National Institutes of Health: K08DE023552National Institute of Dental and Craniofacial Research: R01DE026578National Institutes of Health: R01DE026578University of MichiganFederal University of PelotasFederal University of Rio Grande do SulUniversidade Estadual Paulista (UNESP)University of Michigan School of DentistryRibeiro, Juliana S.Münchow, Eliseu A.Bordini, Ester A. F. [UNESP]Rodrigues, Nathalie S.Dubey, NileshkumarSasaki, HajimeFenno, John C.Schwendeman, StevenBottino, Marco C.2022-04-28T19:49:34Z2022-04-28T19:49:34Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms23020971International Journal of Molecular Sciences, v. 23, n. 2, 2022.1422-00671661-6596http://hdl.handle.net/11449/22325310.3390/ijms230209712-s2.0-85122821177Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2022-04-28T19:49:34Zoai:repositorio.unesp.br:11449/223253Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:42:37.011186Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation |
title |
Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation |
spellingShingle |
Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation Ribeiro, Juliana S. Antibiotics Biodegradation Cryomilling Dentistry Electrospinning Endodontics Fibrous particles Regeneration |
title_short |
Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation |
title_full |
Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation |
title_fullStr |
Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation |
title_full_unstemmed |
Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation |
title_sort |
Engineering of Injectable Antibiotic-Laden Fibrous Microparticles Gelatin Methacryloyl Hydrogel for Endodontic Infection Ablation |
author |
Ribeiro, Juliana S. |
author_facet |
Ribeiro, Juliana S. Münchow, Eliseu A. Bordini, Ester A. F. [UNESP] Rodrigues, Nathalie S. Dubey, Nileshkumar Sasaki, Hajime Fenno, John C. Schwendeman, Steven Bottino, Marco C. |
author_role |
author |
author2 |
Münchow, Eliseu A. Bordini, Ester A. F. [UNESP] Rodrigues, Nathalie S. Dubey, Nileshkumar Sasaki, Hajime Fenno, John C. Schwendeman, Steven Bottino, Marco C. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Michigan Federal University of Pelotas Federal University of Rio Grande do Sul Universidade Estadual Paulista (UNESP) University of Michigan School of Dentistry |
dc.contributor.author.fl_str_mv |
Ribeiro, Juliana S. Münchow, Eliseu A. Bordini, Ester A. F. [UNESP] Rodrigues, Nathalie S. Dubey, Nileshkumar Sasaki, Hajime Fenno, John C. Schwendeman, Steven Bottino, Marco C. |
dc.subject.por.fl_str_mv |
Antibiotics Biodegradation Cryomilling Dentistry Electrospinning Endodontics Fibrous particles Regeneration |
topic |
Antibiotics Biodegradation Cryomilling Dentistry Electrospinning Endodontics Fibrous particles Regeneration |
description |
This study aimed at engineering cytocompatible and injectable antibiotic-laden fibrous microparticles gelatin methacryloyl (GelMA) hydrogels for endodontic infection ablation. Clindamycin (CLIN) or metronidazole (MET) was added to a polymer solution and electrospun into fibrous mats, which were processed via cryomilling to obtain CLIN-or MET-laden fibrous microparticles. Then, GelMA was modified with CLIN-or MET-laden microparticles or by using equal amounts of each set of fibrous microparticles. Morphological characterization of electrospun fibers and cryomilled particles was performed via scanning electron microscopy (SEM). The experimental hydrogels were further examined for swelling, degradation, and toxicity to dental stem cells, as well as antimicrobial action against endodontic pathogens (agar diffusion) and biofilm inhibition, evaluated both quantitatively (CFU/mL) and qualitatively via confocal laser scanning microscopy (CLSM) and SEM. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). The modification of GelMA with antibiotic-laden fibrous microparticles increased the hydrogel swelling ratio and degradation rate. Cell viability was slightly reduced, although without any significant toxicity (cell viability > 50%). All hydrogels containing antibiotic-laden fibrous microparticles displayed antibiofilm effects, with the dentin substrate showing nearly complete elimination of viable bacteria. Altogether, our findings suggest that the engineered injectable antibiotic-laden fibrous microparticles hydrogels hold clinical prospects for endodontic infection ablation. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:49:34Z 2022-04-28T19:49:34Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms23020971 International Journal of Molecular Sciences, v. 23, n. 2, 2022. 1422-0067 1661-6596 http://hdl.handle.net/11449/223253 10.3390/ijms23020971 2-s2.0-85122821177 |
url |
http://dx.doi.org/10.3390/ijms23020971 http://hdl.handle.net/11449/223253 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 23, n. 2, 2022. 1422-0067 1661-6596 10.3390/ijms23020971 2-s2.0-85122821177 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128406809739264 |