Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix

Detalhes bibliográficos
Autor(a) principal: Longatto Filho, Adhemar [UNESP]
Data de Publicação: 1997
Outros Autores: Sakamoto Maeda, Marina Yoshie [UNESP], Oyafuso, Marina Suheko [UNESP], Alves, Venancio Avancini Ferreira [UNESP], Kanamura, Cristina Takami [UNESP], Bortolan, Jeni [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1159/000332791
http://hdl.handle.net/11449/224532
Resumo: OBJECTIVE: To report the cytomorphologic evidence of human papillomavirus (HPV) infection in cervical smears from women treated with radiotherapy for carcinoma of the uterine cervix. STUDY DESIGN: From January 1986 to December 1993, 32 cervical cytologic smears (CCSs) from women who had received radiotherapy (Rtx) for squamous cell cervical carcinoma at A. C. CamargoCancer Hospital were selected for study due to the presence of signs of HPV infection. Review of the files of these patients showed 22 additional samples, which we included in this study. The avidinbiotin- immunoperoxidase technique was performed on previously stained slides using polyclonal rabbit antibovine papillomavirus type 1 to confirm the HPV infection. RESULTS: Positive reactions were found in 18 cases (27 samples), mainly in cells with classic koilocytotic features, but also in epithelial cells that lacked cytopathic effects. Only one case, a biopsy from a verrucous lesion observed after Rtx, was found to be positive for HPV 6/11 DNA by in situ hybridization. CONCLUSION: Cytologic and immunohistochemical positivity for HPV, as well as the reaction with a HPV 6/11 DNA probe, strongly suggested that HPV infection was acquired or expressed after radiotherapy. Further studies are needed to assess the mechanisms responsible forsuch an association. © 1997 The International Academy of Cytology Acta Cytologica.
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spelling Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine CervixCervix neoplasmsHumanPapillomavirusRadiotherapyOBJECTIVE: To report the cytomorphologic evidence of human papillomavirus (HPV) infection in cervical smears from women treated with radiotherapy for carcinoma of the uterine cervix. STUDY DESIGN: From January 1986 to December 1993, 32 cervical cytologic smears (CCSs) from women who had received radiotherapy (Rtx) for squamous cell cervical carcinoma at A. C. CamargoCancer Hospital were selected for study due to the presence of signs of HPV infection. Review of the files of these patients showed 22 additional samples, which we included in this study. The avidinbiotin- immunoperoxidase technique was performed on previously stained slides using polyclonal rabbit antibovine papillomavirus type 1 to confirm the HPV infection. RESULTS: Positive reactions were found in 18 cases (27 samples), mainly in cells with classic koilocytotic features, but also in epithelial cells that lacked cytopathic effects. Only one case, a biopsy from a verrucous lesion observed after Rtx, was found to be positive for HPV 6/11 DNA by in situ hybridization. CONCLUSION: Cytologic and immunohistochemical positivity for HPV, as well as the reaction with a HPV 6/11 DNA probe, strongly suggested that HPV infection was acquired or expressed after radiotherapy. Further studies are needed to assess the mechanisms responsible forsuch an association. © 1997 The International Academy of Cytology Acta Cytologica.Pathology Division Adolfo Lutz InstitutePathology Department A. C. Camargo HospitalDepartment of Pathology São Paulo State University Medical School, São PauloA. C. Camargo Hospital Adolfo Lutz InstituteAdolfo Lutz Institute São Paulo State University Medical SchoolA. C. Camargo HospitalDepartment of Pathology São Paulo State University Medical School, São PauloAdolfo Lutz Institute São Paulo State University Medical SchoolAdolfo Lutz InstituteA. C. Camargo HospitalUniversidade Estadual Paulista (UNESP)Longatto Filho, Adhemar [UNESP]Sakamoto Maeda, Marina Yoshie [UNESP]Oyafuso, Marina Suheko [UNESP]Alves, Venancio Avancini Ferreira [UNESP]Kanamura, Cristina Takami [UNESP]Bortolan, Jeni [UNESP]2022-04-28T19:59:54Z2022-04-28T19:59:54Z1997-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1079-1084http://dx.doi.org/10.1159/000332791Acta Cytologica, v. 41, n. 4, p. 1079-1084, 1997.1938-26500001-5547http://hdl.handle.net/11449/22453210.1159/0003327912-s2.0-1842332075Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cytologicainfo:eu-repo/semantics/openAccess2024-09-03T13:18:23Zoai:repositorio.unesp.br:11449/224532Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix
title Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix
spellingShingle Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix
Longatto Filho, Adhemar [UNESP]
Cervix neoplasms
Human
Papillomavirus
Radiotherapy
title_short Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix
title_full Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix
title_fullStr Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix
title_full_unstemmed Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix
title_sort Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix
author Longatto Filho, Adhemar [UNESP]
author_facet Longatto Filho, Adhemar [UNESP]
Sakamoto Maeda, Marina Yoshie [UNESP]
Oyafuso, Marina Suheko [UNESP]
Alves, Venancio Avancini Ferreira [UNESP]
Kanamura, Cristina Takami [UNESP]
Bortolan, Jeni [UNESP]
author_role author
author2 Sakamoto Maeda, Marina Yoshie [UNESP]
Oyafuso, Marina Suheko [UNESP]
Alves, Venancio Avancini Ferreira [UNESP]
Kanamura, Cristina Takami [UNESP]
Bortolan, Jeni [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Adolfo Lutz Institute
A. C. Camargo Hospital
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Longatto Filho, Adhemar [UNESP]
Sakamoto Maeda, Marina Yoshie [UNESP]
Oyafuso, Marina Suheko [UNESP]
Alves, Venancio Avancini Ferreira [UNESP]
Kanamura, Cristina Takami [UNESP]
Bortolan, Jeni [UNESP]
dc.subject.por.fl_str_mv Cervix neoplasms
Human
Papillomavirus
Radiotherapy
topic Cervix neoplasms
Human
Papillomavirus
Radiotherapy
description OBJECTIVE: To report the cytomorphologic evidence of human papillomavirus (HPV) infection in cervical smears from women treated with radiotherapy for carcinoma of the uterine cervix. STUDY DESIGN: From January 1986 to December 1993, 32 cervical cytologic smears (CCSs) from women who had received radiotherapy (Rtx) for squamous cell cervical carcinoma at A. C. CamargoCancer Hospital were selected for study due to the presence of signs of HPV infection. Review of the files of these patients showed 22 additional samples, which we included in this study. The avidinbiotin- immunoperoxidase technique was performed on previously stained slides using polyclonal rabbit antibovine papillomavirus type 1 to confirm the HPV infection. RESULTS: Positive reactions were found in 18 cases (27 samples), mainly in cells with classic koilocytotic features, but also in epithelial cells that lacked cytopathic effects. Only one case, a biopsy from a verrucous lesion observed after Rtx, was found to be positive for HPV 6/11 DNA by in situ hybridization. CONCLUSION: Cytologic and immunohistochemical positivity for HPV, as well as the reaction with a HPV 6/11 DNA probe, strongly suggested that HPV infection was acquired or expressed after radiotherapy. Further studies are needed to assess the mechanisms responsible forsuch an association. © 1997 The International Academy of Cytology Acta Cytologica.
publishDate 1997
dc.date.none.fl_str_mv 1997-01-01
2022-04-28T19:59:54Z
2022-04-28T19:59:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000332791
Acta Cytologica, v. 41, n. 4, p. 1079-1084, 1997.
1938-2650
0001-5547
http://hdl.handle.net/11449/224532
10.1159/000332791
2-s2.0-1842332075
url http://dx.doi.org/10.1159/000332791
http://hdl.handle.net/11449/224532
identifier_str_mv Acta Cytologica, v. 41, n. 4, p. 1079-1084, 1997.
1938-2650
0001-5547
10.1159/000332791
2-s2.0-1842332075
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cytologica
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1079-1084
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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