Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000332791 http://hdl.handle.net/11449/224532 |
Resumo: | OBJECTIVE: To report the cytomorphologic evidence of human papillomavirus (HPV) infection in cervical smears from women treated with radiotherapy for carcinoma of the uterine cervix. STUDY DESIGN: From January 1986 to December 1993, 32 cervical cytologic smears (CCSs) from women who had received radiotherapy (Rtx) for squamous cell cervical carcinoma at A. C. CamargoCancer Hospital were selected for study due to the presence of signs of HPV infection. Review of the files of these patients showed 22 additional samples, which we included in this study. The avidinbiotin- immunoperoxidase technique was performed on previously stained slides using polyclonal rabbit antibovine papillomavirus type 1 to confirm the HPV infection. RESULTS: Positive reactions were found in 18 cases (27 samples), mainly in cells with classic koilocytotic features, but also in epithelial cells that lacked cytopathic effects. Only one case, a biopsy from a verrucous lesion observed after Rtx, was found to be positive for HPV 6/11 DNA by in situ hybridization. CONCLUSION: Cytologic and immunohistochemical positivity for HPV, as well as the reaction with a HPV 6/11 DNA probe, strongly suggested that HPV infection was acquired or expressed after radiotherapy. Further studies are needed to assess the mechanisms responsible forsuch an association. © 1997 The International Academy of Cytology Acta Cytologica. |
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Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine CervixCervix neoplasmsHumanPapillomavirusRadiotherapyOBJECTIVE: To report the cytomorphologic evidence of human papillomavirus (HPV) infection in cervical smears from women treated with radiotherapy for carcinoma of the uterine cervix. STUDY DESIGN: From January 1986 to December 1993, 32 cervical cytologic smears (CCSs) from women who had received radiotherapy (Rtx) for squamous cell cervical carcinoma at A. C. CamargoCancer Hospital were selected for study due to the presence of signs of HPV infection. Review of the files of these patients showed 22 additional samples, which we included in this study. The avidinbiotin- immunoperoxidase technique was performed on previously stained slides using polyclonal rabbit antibovine papillomavirus type 1 to confirm the HPV infection. RESULTS: Positive reactions were found in 18 cases (27 samples), mainly in cells with classic koilocytotic features, but also in epithelial cells that lacked cytopathic effects. Only one case, a biopsy from a verrucous lesion observed after Rtx, was found to be positive for HPV 6/11 DNA by in situ hybridization. CONCLUSION: Cytologic and immunohistochemical positivity for HPV, as well as the reaction with a HPV 6/11 DNA probe, strongly suggested that HPV infection was acquired or expressed after radiotherapy. Further studies are needed to assess the mechanisms responsible forsuch an association. © 1997 The International Academy of Cytology Acta Cytologica.Pathology Division Adolfo Lutz InstitutePathology Department A. C. Camargo HospitalDepartment of Pathology São Paulo State University Medical School, São PauloA. C. Camargo Hospital Adolfo Lutz InstituteAdolfo Lutz Institute São Paulo State University Medical SchoolA. C. Camargo HospitalDepartment of Pathology São Paulo State University Medical School, São PauloAdolfo Lutz Institute São Paulo State University Medical SchoolAdolfo Lutz InstituteA. C. Camargo HospitalUniversidade Estadual Paulista (UNESP)Longatto Filho, Adhemar [UNESP]Sakamoto Maeda, Marina Yoshie [UNESP]Oyafuso, Marina Suheko [UNESP]Alves, Venancio Avancini Ferreira [UNESP]Kanamura, Cristina Takami [UNESP]Bortolan, Jeni [UNESP]2022-04-28T19:59:54Z2022-04-28T19:59:54Z1997-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1079-1084http://dx.doi.org/10.1159/000332791Acta Cytologica, v. 41, n. 4, p. 1079-1084, 1997.1938-26500001-5547http://hdl.handle.net/11449/22453210.1159/0003327912-s2.0-1842332075Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cytologicainfo:eu-repo/semantics/openAccess2024-09-03T13:18:23Zoai:repositorio.unesp.br:11449/224532Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix |
title |
Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix |
spellingShingle |
Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix Longatto Filho, Adhemar [UNESP] Cervix neoplasms Human Papillomavirus Radiotherapy |
title_short |
Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix |
title_full |
Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix |
title_fullStr |
Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix |
title_full_unstemmed |
Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix |
title_sort |
Cytomorphologic Evidenceof Human Papillomavirus Infection in Smears from the IrradiatedUterine Cervix |
author |
Longatto Filho, Adhemar [UNESP] |
author_facet |
Longatto Filho, Adhemar [UNESP] Sakamoto Maeda, Marina Yoshie [UNESP] Oyafuso, Marina Suheko [UNESP] Alves, Venancio Avancini Ferreira [UNESP] Kanamura, Cristina Takami [UNESP] Bortolan, Jeni [UNESP] |
author_role |
author |
author2 |
Sakamoto Maeda, Marina Yoshie [UNESP] Oyafuso, Marina Suheko [UNESP] Alves, Venancio Avancini Ferreira [UNESP] Kanamura, Cristina Takami [UNESP] Bortolan, Jeni [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Adolfo Lutz Institute A. C. Camargo Hospital Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Longatto Filho, Adhemar [UNESP] Sakamoto Maeda, Marina Yoshie [UNESP] Oyafuso, Marina Suheko [UNESP] Alves, Venancio Avancini Ferreira [UNESP] Kanamura, Cristina Takami [UNESP] Bortolan, Jeni [UNESP] |
dc.subject.por.fl_str_mv |
Cervix neoplasms Human Papillomavirus Radiotherapy |
topic |
Cervix neoplasms Human Papillomavirus Radiotherapy |
description |
OBJECTIVE: To report the cytomorphologic evidence of human papillomavirus (HPV) infection in cervical smears from women treated with radiotherapy for carcinoma of the uterine cervix. STUDY DESIGN: From January 1986 to December 1993, 32 cervical cytologic smears (CCSs) from women who had received radiotherapy (Rtx) for squamous cell cervical carcinoma at A. C. CamargoCancer Hospital were selected for study due to the presence of signs of HPV infection. Review of the files of these patients showed 22 additional samples, which we included in this study. The avidinbiotin- immunoperoxidase technique was performed on previously stained slides using polyclonal rabbit antibovine papillomavirus type 1 to confirm the HPV infection. RESULTS: Positive reactions were found in 18 cases (27 samples), mainly in cells with classic koilocytotic features, but also in epithelial cells that lacked cytopathic effects. Only one case, a biopsy from a verrucous lesion observed after Rtx, was found to be positive for HPV 6/11 DNA by in situ hybridization. CONCLUSION: Cytologic and immunohistochemical positivity for HPV, as well as the reaction with a HPV 6/11 DNA probe, strongly suggested that HPV infection was acquired or expressed after radiotherapy. Further studies are needed to assess the mechanisms responsible forsuch an association. © 1997 The International Academy of Cytology Acta Cytologica. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-01-01 2022-04-28T19:59:54Z 2022-04-28T19:59:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000332791 Acta Cytologica, v. 41, n. 4, p. 1079-1084, 1997. 1938-2650 0001-5547 http://hdl.handle.net/11449/224532 10.1159/000332791 2-s2.0-1842332075 |
url |
http://dx.doi.org/10.1159/000332791 http://hdl.handle.net/11449/224532 |
identifier_str_mv |
Acta Cytologica, v. 41, n. 4, p. 1079-1084, 1997. 1938-2650 0001-5547 10.1159/000332791 2-s2.0-1842332075 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cytologica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1079-1084 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021411670982656 |