Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, Brazil

Detalhes bibliográficos
Autor(a) principal: Munhoz, Alexandre D.
Data de Publicação: 2011
Outros Autores: Jacintho, Ana P. P. [UNESP], Machado, Rosangela Z. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-20-20881_2011_7_5_39_32.pdf
http://hdl.handle.net/11449/72545
Resumo: The objective of this study was to report the presence of Neospora caninum-associated abortion in bovines at a farm in the northeast region of São Paulo State. In January 2010, it was sent to the Department of Pathology, UNESP-Jaboticabal, a bovine fetus with an estimated age of seven months, which was natural of a dairy farm with 300 animals and an average daily production of 3,000 liters of milk, nearly 20 liters per cow. The animals were vaccinated against rabies, foot and mouth disease, carbuncle, brucellosis, leptospirosis, bovine herpes virus type I and bovine viral diarrhea virus. The herd consisted of purebred Holstein animals, Jersey, and mostly by crossbred animals 7-8 (gir x holstein). During necropsy, samples of the serosanguineous liquid present at the thoracic cavity and the heart of the fetus were collected for the detection of anti-Neospora caninum antibodies through Indirect Immunofluorescence Assay (IFA). Fragments of brain, cerebellum, tongue, liver, heart and kidneys were collected for the execution of histopathology (HP), immunohistochemistry (IHC) and Polymerase Chain Reaction. In order that IFA could be performed, the owner was requested blood samples without anticoagulants of the mother and other cows in the farm, with or without a history of abortion. At necropsy, it was verified a severe autolysis of the fetus. The serology of the fetus was 1:25, while the serology of the mother was 1:3,200. At HP, it was observed discrete multifocal non-suppurative encephalomyelitis characterized by gliosis and mononuclear inflammatory infiltration associated with cellular debris. DNA amplification of N. caninum was positive in fragments of brain, tongue, cerebellum, heart and kidneys. At IHC, it has been observed immunoreactivity to a cyst located in the tongue. The owner reported that his herd showed endemic episodes of abortion, while 27.69% (18/65) of the 65 animals sampled were seropositive. Although it has not been a significant difference (p>0.05), a higher seropositivity was observed in animals with a history of abortion (10/26) 38.46%, in comparison with animals without previous abortion (8/39) 20.51%. These findings show that the abortion under study was provoked by the protozoan N. caninum, while this is the first report concerning cattle in the northeast region of São Paulo State.
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spelling Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, BrazilAbortionCattleNeosporosisAnimaliaBosBovinaeBovine viral diarrhea virus 1HerpesviridaeNeospora caninumProtozoaThe objective of this study was to report the presence of Neospora caninum-associated abortion in bovines at a farm in the northeast region of São Paulo State. In January 2010, it was sent to the Department of Pathology, UNESP-Jaboticabal, a bovine fetus with an estimated age of seven months, which was natural of a dairy farm with 300 animals and an average daily production of 3,000 liters of milk, nearly 20 liters per cow. The animals were vaccinated against rabies, foot and mouth disease, carbuncle, brucellosis, leptospirosis, bovine herpes virus type I and bovine viral diarrhea virus. The herd consisted of purebred Holstein animals, Jersey, and mostly by crossbred animals 7-8 (gir x holstein). During necropsy, samples of the serosanguineous liquid present at the thoracic cavity and the heart of the fetus were collected for the detection of anti-Neospora caninum antibodies through Indirect Immunofluorescence Assay (IFA). Fragments of brain, cerebellum, tongue, liver, heart and kidneys were collected for the execution of histopathology (HP), immunohistochemistry (IHC) and Polymerase Chain Reaction. In order that IFA could be performed, the owner was requested blood samples without anticoagulants of the mother and other cows in the farm, with or without a history of abortion. At necropsy, it was verified a severe autolysis of the fetus. The serology of the fetus was 1:25, while the serology of the mother was 1:3,200. At HP, it was observed discrete multifocal non-suppurative encephalomyelitis characterized by gliosis and mononuclear inflammatory infiltration associated with cellular debris. DNA amplification of N. caninum was positive in fragments of brain, tongue, cerebellum, heart and kidneys. At IHC, it has been observed immunoreactivity to a cyst located in the tongue. The owner reported that his herd showed endemic episodes of abortion, while 27.69% (18/65) of the 65 animals sampled were seropositive. Although it has not been a significant difference (p>0.05), a higher seropositivity was observed in animals with a history of abortion (10/26) 38.46%, in comparison with animals without previous abortion (8/39) 20.51%. These findings show that the abortion under study was provoked by the protozoan N. caninum, while this is the first report concerning cattle in the northeast region of São Paulo State.Universidade Estadual de Santa Cruz (UESC) Departamento de Ciências Agrárias e Ambientais, Campus Soane Nazaré de Andrade Ilhéus, BahiaUNESP Universidade Estadual Paulista Depto. de Patologia Veterinária, Jaboticabal, São PauloUNESP Universidade Estadual Paulista Depto. de Patologia Veterinária, Jaboticabal, São PauloUniversidade Estadual de Santa Cruz (UESC)Universidade Estadual Paulista (Unesp)Munhoz, Alexandre D.Jacintho, Ana P. P. [UNESP]Machado, Rosangela Z. [UNESP]2014-05-27T11:25:56Z2014-05-27T11:25:56Z2011-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article112-116http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-20-20881_2011_7_5_39_32.pdfBrazilian Journal of Veterinary Pathology, v. 4, n. 2, p. 112-116, 2011.1983-0246http://hdl.handle.net/11449/725452-s2.0-79960684910Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Veterinary Pathology0,162info:eu-repo/semantics/openAccess2024-06-07T13:01:44Zoai:repositorio.unesp.br:11449/72545Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:11:40.212892Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, Brazil
title Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, Brazil
spellingShingle Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, Brazil
Munhoz, Alexandre D.
Abortion
Cattle
Neosporosis
Animalia
Bos
Bovinae
Bovine viral diarrhea virus 1
Herpesviridae
Neospora caninum
Protozoa
title_short Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, Brazil
title_full Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, Brazil
title_fullStr Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, Brazil
title_full_unstemmed Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, Brazil
title_sort Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the northeast region of São Paulo state, Brazil
author Munhoz, Alexandre D.
author_facet Munhoz, Alexandre D.
Jacintho, Ana P. P. [UNESP]
Machado, Rosangela Z. [UNESP]
author_role author
author2 Jacintho, Ana P. P. [UNESP]
Machado, Rosangela Z. [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Santa Cruz (UESC)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Munhoz, Alexandre D.
Jacintho, Ana P. P. [UNESP]
Machado, Rosangela Z. [UNESP]
dc.subject.por.fl_str_mv Abortion
Cattle
Neosporosis
Animalia
Bos
Bovinae
Bovine viral diarrhea virus 1
Herpesviridae
Neospora caninum
Protozoa
topic Abortion
Cattle
Neosporosis
Animalia
Bos
Bovinae
Bovine viral diarrhea virus 1
Herpesviridae
Neospora caninum
Protozoa
description The objective of this study was to report the presence of Neospora caninum-associated abortion in bovines at a farm in the northeast region of São Paulo State. In January 2010, it was sent to the Department of Pathology, UNESP-Jaboticabal, a bovine fetus with an estimated age of seven months, which was natural of a dairy farm with 300 animals and an average daily production of 3,000 liters of milk, nearly 20 liters per cow. The animals were vaccinated against rabies, foot and mouth disease, carbuncle, brucellosis, leptospirosis, bovine herpes virus type I and bovine viral diarrhea virus. The herd consisted of purebred Holstein animals, Jersey, and mostly by crossbred animals 7-8 (gir x holstein). During necropsy, samples of the serosanguineous liquid present at the thoracic cavity and the heart of the fetus were collected for the detection of anti-Neospora caninum antibodies through Indirect Immunofluorescence Assay (IFA). Fragments of brain, cerebellum, tongue, liver, heart and kidneys were collected for the execution of histopathology (HP), immunohistochemistry (IHC) and Polymerase Chain Reaction. In order that IFA could be performed, the owner was requested blood samples without anticoagulants of the mother and other cows in the farm, with or without a history of abortion. At necropsy, it was verified a severe autolysis of the fetus. The serology of the fetus was 1:25, while the serology of the mother was 1:3,200. At HP, it was observed discrete multifocal non-suppurative encephalomyelitis characterized by gliosis and mononuclear inflammatory infiltration associated with cellular debris. DNA amplification of N. caninum was positive in fragments of brain, tongue, cerebellum, heart and kidneys. At IHC, it has been observed immunoreactivity to a cyst located in the tongue. The owner reported that his herd showed endemic episodes of abortion, while 27.69% (18/65) of the 65 animals sampled were seropositive. Although it has not been a significant difference (p>0.05), a higher seropositivity was observed in animals with a history of abortion (10/26) 38.46%, in comparison with animals without previous abortion (8/39) 20.51%. These findings show that the abortion under study was provoked by the protozoan N. caninum, while this is the first report concerning cattle in the northeast region of São Paulo State.
publishDate 2011
dc.date.none.fl_str_mv 2011-07-01
2014-05-27T11:25:56Z
2014-05-27T11:25:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-20-20881_2011_7_5_39_32.pdf
Brazilian Journal of Veterinary Pathology, v. 4, n. 2, p. 112-116, 2011.
1983-0246
http://hdl.handle.net/11449/72545
2-s2.0-79960684910
url http://www.abpv.vet.br/upload/documentos/DOWNLOAD-FULL-ARTICLE-20-20881_2011_7_5_39_32.pdf
http://hdl.handle.net/11449/72545
identifier_str_mv Brazilian Journal of Veterinary Pathology, v. 4, n. 2, p. 112-116, 2011.
1983-0246
2-s2.0-79960684910
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Veterinary Pathology
0,162
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 112-116
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
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