MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3732/ajb.1200080 http://hdl.handle.net/11449/39854 |
Resumo: | Premise of the study: Microsatellite markers were developed to study the genetic diversity and population structure of the carnivorous bladderwort Utricularia reniformis, which is endemic to the Atlantic Forest of southern and southeastern Brazil. Cross-species amplification was tested in U. gibba, U. neottioides, U. subulata, and Pinguicula benedicta.Methods and Results: The U. reniformis genome was sequenced in a 454 GS FLX sequencer, and eight primer sets were developed based on the microsatellites identified from the reads. All loci are polymorphic, showing 1.6 to 4.8 alleles per population. Preliminary results show that primer sets are suitable for population-level studies. Cross-species amplifi cation was successful in three other Utricularia species and one Pinguicula species.Conclusions: Markers developed in this study provide tools for analyses of intra- and interpopulation genetic diversity in Utricularia and Pinguicula. |
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Repositório Institucional da UNESP |
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spelling |
MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE)Atlantic Forestbladderwortsendemic plantsgenetic diversityLentibulariaceaeUtricularia reniformisPremise of the study: Microsatellite markers were developed to study the genetic diversity and population structure of the carnivorous bladderwort Utricularia reniformis, which is endemic to the Atlantic Forest of southern and southeastern Brazil. Cross-species amplification was tested in U. gibba, U. neottioides, U. subulata, and Pinguicula benedicta.Methods and Results: The U. reniformis genome was sequenced in a 454 GS FLX sequencer, and eight primer sets were developed based on the microsatellites identified from the reads. All loci are polymorphic, showing 1.6 to 4.8 alleles per population. Preliminary results show that primer sets are suitable for population-level studies. Cross-species amplifi cation was successful in three other Utricularia species and one Pinguicula species.Conclusions: Markers developed in this study provide tools for analyses of intra- and interpopulation genetic diversity in Utricularia and Pinguicula.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, BR-08780911 São Paulo, BrazilUniv Florida, Dept Biol, Gainesville, FL 32611 USAUniv São Paulo, Dept Microbiol, BR-05508900 São Paulo, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 São Paulo, BrazilFAPESP: 08/22239-9FAPESP: 07/58277-7CAPES: 5316/10-3Botanical Soc Amer IncUniv Mogi das CruzesUniv FloridaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Clivati, DeboraGitzendanner, Matthew AaronSilva Hilsdorf, Alexandre WagnerAraujo, Welington LuizOliveira de Miranda, Vitor Fernandes [UNESP]2014-05-20T15:30:30Z2014-05-20T15:30:30Z2012-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleE375-E378http://dx.doi.org/10.3732/ajb.1200080American Journal of Botany. St Louis: Botanical Soc Amer Inc, v. 99, n. 9, p. E375-E378, 2012.0002-9122http://hdl.handle.net/11449/3985410.3732/ajb.1200080WOS:000308689800011Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAmerican Journal of Botany2.7881,499info:eu-repo/semantics/openAccess2021-10-23T10:40:55Zoai:repositorio.unesp.br:11449/39854Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:40:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE) |
title |
MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE) |
spellingShingle |
MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE) Clivati, Debora Atlantic Forest bladderworts endemic plants genetic diversity Lentibulariaceae Utricularia reniformis |
title_short |
MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE) |
title_full |
MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE) |
title_fullStr |
MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE) |
title_full_unstemmed |
MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE) |
title_sort |
MICROSATELLITE MARKERS DEVELOPED FOR UTRICULARIA RENIFORMIS (LENTIBULARIACEAE) |
author |
Clivati, Debora |
author_facet |
Clivati, Debora Gitzendanner, Matthew Aaron Silva Hilsdorf, Alexandre Wagner Araujo, Welington Luiz Oliveira de Miranda, Vitor Fernandes [UNESP] |
author_role |
author |
author2 |
Gitzendanner, Matthew Aaron Silva Hilsdorf, Alexandre Wagner Araujo, Welington Luiz Oliveira de Miranda, Vitor Fernandes [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Univ Mogi das Cruzes Univ Florida Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Clivati, Debora Gitzendanner, Matthew Aaron Silva Hilsdorf, Alexandre Wagner Araujo, Welington Luiz Oliveira de Miranda, Vitor Fernandes [UNESP] |
dc.subject.por.fl_str_mv |
Atlantic Forest bladderworts endemic plants genetic diversity Lentibulariaceae Utricularia reniformis |
topic |
Atlantic Forest bladderworts endemic plants genetic diversity Lentibulariaceae Utricularia reniformis |
description |
Premise of the study: Microsatellite markers were developed to study the genetic diversity and population structure of the carnivorous bladderwort Utricularia reniformis, which is endemic to the Atlantic Forest of southern and southeastern Brazil. Cross-species amplification was tested in U. gibba, U. neottioides, U. subulata, and Pinguicula benedicta.Methods and Results: The U. reniformis genome was sequenced in a 454 GS FLX sequencer, and eight primer sets were developed based on the microsatellites identified from the reads. All loci are polymorphic, showing 1.6 to 4.8 alleles per population. Preliminary results show that primer sets are suitable for population-level studies. Cross-species amplifi cation was successful in three other Utricularia species and one Pinguicula species.Conclusions: Markers developed in this study provide tools for analyses of intra- and interpopulation genetic diversity in Utricularia and Pinguicula. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 2014-05-20T15:30:30Z 2014-05-20T15:30:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3732/ajb.1200080 American Journal of Botany. St Louis: Botanical Soc Amer Inc, v. 99, n. 9, p. E375-E378, 2012. 0002-9122 http://hdl.handle.net/11449/39854 10.3732/ajb.1200080 WOS:000308689800011 |
url |
http://dx.doi.org/10.3732/ajb.1200080 http://hdl.handle.net/11449/39854 |
identifier_str_mv |
American Journal of Botany. St Louis: Botanical Soc Amer Inc, v. 99, n. 9, p. E375-E378, 2012. 0002-9122 10.3732/ajb.1200080 WOS:000308689800011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
American Journal of Botany 2.788 1,499 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
E375-E378 |
dc.publisher.none.fl_str_mv |
Botanical Soc Amer Inc |
publisher.none.fl_str_mv |
Botanical Soc Amer Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965470486429696 |