In vitro effect of low-level laser therapy on typical oral microbial biofilms
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0103-64402011000600011 http://hdl.handle.net/11449/73110 |
Resumo: | The aim of this study was to evaluate the effect of specific parameters of low-level laser therapy (LLLT) on biofilms formed by Streptococcus mutans, Candida albicans or an association of both species. Single and dual-species biofilms - SSB and DSB - were exposed to laser doses of 5, 10 or 20 J/cm 2 from a near infrared InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm, 0.04 W). After irradiation, the analysis of biobilm viability (MTT assay), biofilm growth (cfu/mL) and cell morphology (SEM) showed that LLLT reduced cell viability as well as the growth of biofilms. The response of S. mutans (SSB) to irradiation was similar for all laser doses and the biofilm growth was dose dependent. However, when associated with C. albicans (DSB), S. mutans was resistant to LLLT. For C. albicans, the association with S. mutans (DSB) caused a significant decrease in biofilm growth in a dose-dependent fashion. The morphology of the microorganisms in the SSB was not altered by LLLT, while the association of microbial species (DSB) promoted a reduction in the formation of C. albicans hyphae. LLLT had an inhibitory effect on the microorganisms, and this capacity can be altered according to the interactions between different microbial species. |
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In vitro effect of low-level laser therapy on typical oral microbial biofilmsBiofilmCandida albicansLow level laser therapyStreptococcus mutanscoloring agentdiagnostic agentsuccinate dehydrogenasetetrazoliumthiazole derivativethiazolyl bluebiofilmcomparative studydiode laserfungus hyphaegrowth, development and aginghumaninstrumentationlow level laser therapymaterials testingmethodologymicrobial viabilitymicrobiological examinationmicrobiologymouthmycologyorganismal interactionradiation doseradiation exposureradiation responsescanning electron microscopytemperaturetimeultrastructureBacteriological TechniquesBiofilmsColoring AgentsDose-Response Relationship, RadiationHumansHyphaeLaser Therapy, Low-LevelLasers, SemiconductorMaterials TestingMicrobial InteractionsMicrobial ViabilityMicroscopy, Electron, ScanningMouthMycologyRadiation DosageSuccinate DehydrogenaseTemperatureTetrazolium SaltsThiazolesTime FactorsThe aim of this study was to evaluate the effect of specific parameters of low-level laser therapy (LLLT) on biofilms formed by Streptococcus mutans, Candida albicans or an association of both species. Single and dual-species biofilms - SSB and DSB - were exposed to laser doses of 5, 10 or 20 J/cm 2 from a near infrared InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm, 0.04 W). After irradiation, the analysis of biobilm viability (MTT assay), biofilm growth (cfu/mL) and cell morphology (SEM) showed that LLLT reduced cell viability as well as the growth of biofilms. The response of S. mutans (SSB) to irradiation was similar for all laser doses and the biofilm growth was dose dependent. However, when associated with C. albicans (DSB), S. mutans was resistant to LLLT. For C. albicans, the association with S. mutans (DSB) caused a significant decrease in biofilm growth in a dose-dependent fashion. The morphology of the microorganisms in the SSB was not altered by LLLT, while the association of microbial species (DSB) promoted a reduction in the formation of C. albicans hyphae. LLLT had an inhibitory effect on the microorganisms, and this capacity can be altered according to the interactions between different microbial species.Piracicaba Dental School UNICAMP - University of Campinas, Piracicaba, SPAraraquara Dental School UNESP - Univ. Estadual Paulista, Araraquara, SPPhysics Institute of São Carlos USP - University of São Paulo, São Carlos, SPAraraquara Dental School UNESP - Univ. Estadual Paulista, Araraquara, SPUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Basso, Fernanda G.Oliveira, Camila F. [UNESP]Fontana, Amanda [UNESP]Kurachi, CristinaBagnato, Vanderlei S.Spolidório, Denise M. P. [UNESP]Hebling, Josimeri [UNESP]de Souza Costa, Carlos A. [UNESP]2014-05-27T11:26:21Z2014-05-27T11:26:21Z2011-12-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article502-510application/pdfhttp://dx.doi.org/10.1590/S0103-64402011000600011Brazilian Dental Journal, v. 22, n. 6, p. 502-510, 2011.0103-64401806-4760http://hdl.handle.net/11449/7311010.1590/S0103-64402011000600011S0103-644020110006000112-s2.0-842551668142-s2.0-84255166814.pdf4517484241515548Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journal0,476info:eu-repo/semantics/openAccess2023-10-22T06:11:38Zoai:repositorio.unesp.br:11449/73110Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:39:25.554840Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro effect of low-level laser therapy on typical oral microbial biofilms |
title |
In vitro effect of low-level laser therapy on typical oral microbial biofilms |
spellingShingle |
In vitro effect of low-level laser therapy on typical oral microbial biofilms Basso, Fernanda G. Biofilm Candida albicans Low level laser therapy Streptococcus mutans coloring agent diagnostic agent succinate dehydrogenase tetrazolium thiazole derivative thiazolyl blue biofilm comparative study diode laser fungus hyphae growth, development and aging human instrumentation low level laser therapy materials testing methodology microbial viability microbiological examination microbiology mouth mycology organismal interaction radiation dose radiation exposure radiation response scanning electron microscopy temperature time ultrastructure Bacteriological Techniques Biofilms Coloring Agents Dose-Response Relationship, Radiation Humans Hyphae Laser Therapy, Low-Level Lasers, Semiconductor Materials Testing Microbial Interactions Microbial Viability Microscopy, Electron, Scanning Mouth Mycology Radiation Dosage Succinate Dehydrogenase Temperature Tetrazolium Salts Thiazoles Time Factors |
title_short |
In vitro effect of low-level laser therapy on typical oral microbial biofilms |
title_full |
In vitro effect of low-level laser therapy on typical oral microbial biofilms |
title_fullStr |
In vitro effect of low-level laser therapy on typical oral microbial biofilms |
title_full_unstemmed |
In vitro effect of low-level laser therapy on typical oral microbial biofilms |
title_sort |
In vitro effect of low-level laser therapy on typical oral microbial biofilms |
author |
Basso, Fernanda G. |
author_facet |
Basso, Fernanda G. Oliveira, Camila F. [UNESP] Fontana, Amanda [UNESP] Kurachi, Cristina Bagnato, Vanderlei S. Spolidório, Denise M. P. [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos A. [UNESP] |
author_role |
author |
author2 |
Oliveira, Camila F. [UNESP] Fontana, Amanda [UNESP] Kurachi, Cristina Bagnato, Vanderlei S. Spolidório, Denise M. P. [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos A. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Basso, Fernanda G. Oliveira, Camila F. [UNESP] Fontana, Amanda [UNESP] Kurachi, Cristina Bagnato, Vanderlei S. Spolidório, Denise M. P. [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos A. [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Candida albicans Low level laser therapy Streptococcus mutans coloring agent diagnostic agent succinate dehydrogenase tetrazolium thiazole derivative thiazolyl blue biofilm comparative study diode laser fungus hyphae growth, development and aging human instrumentation low level laser therapy materials testing methodology microbial viability microbiological examination microbiology mouth mycology organismal interaction radiation dose radiation exposure radiation response scanning electron microscopy temperature time ultrastructure Bacteriological Techniques Biofilms Coloring Agents Dose-Response Relationship, Radiation Humans Hyphae Laser Therapy, Low-Level Lasers, Semiconductor Materials Testing Microbial Interactions Microbial Viability Microscopy, Electron, Scanning Mouth Mycology Radiation Dosage Succinate Dehydrogenase Temperature Tetrazolium Salts Thiazoles Time Factors |
topic |
Biofilm Candida albicans Low level laser therapy Streptococcus mutans coloring agent diagnostic agent succinate dehydrogenase tetrazolium thiazole derivative thiazolyl blue biofilm comparative study diode laser fungus hyphae growth, development and aging human instrumentation low level laser therapy materials testing methodology microbial viability microbiological examination microbiology mouth mycology organismal interaction radiation dose radiation exposure radiation response scanning electron microscopy temperature time ultrastructure Bacteriological Techniques Biofilms Coloring Agents Dose-Response Relationship, Radiation Humans Hyphae Laser Therapy, Low-Level Lasers, Semiconductor Materials Testing Microbial Interactions Microbial Viability Microscopy, Electron, Scanning Mouth Mycology Radiation Dosage Succinate Dehydrogenase Temperature Tetrazolium Salts Thiazoles Time Factors |
description |
The aim of this study was to evaluate the effect of specific parameters of low-level laser therapy (LLLT) on biofilms formed by Streptococcus mutans, Candida albicans or an association of both species. Single and dual-species biofilms - SSB and DSB - were exposed to laser doses of 5, 10 or 20 J/cm 2 from a near infrared InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm, 0.04 W). After irradiation, the analysis of biobilm viability (MTT assay), biofilm growth (cfu/mL) and cell morphology (SEM) showed that LLLT reduced cell viability as well as the growth of biofilms. The response of S. mutans (SSB) to irradiation was similar for all laser doses and the biofilm growth was dose dependent. However, when associated with C. albicans (DSB), S. mutans was resistant to LLLT. For C. albicans, the association with S. mutans (DSB) caused a significant decrease in biofilm growth in a dose-dependent fashion. The morphology of the microorganisms in the SSB was not altered by LLLT, while the association of microbial species (DSB) promoted a reduction in the formation of C. albicans hyphae. LLLT had an inhibitory effect on the microorganisms, and this capacity can be altered according to the interactions between different microbial species. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-28 2014-05-27T11:26:21Z 2014-05-27T11:26:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0103-64402011000600011 Brazilian Dental Journal, v. 22, n. 6, p. 502-510, 2011. 0103-6440 1806-4760 http://hdl.handle.net/11449/73110 10.1590/S0103-64402011000600011 S0103-64402011000600011 2-s2.0-84255166814 2-s2.0-84255166814.pdf 4517484241515548 |
url |
http://dx.doi.org/10.1590/S0103-64402011000600011 http://hdl.handle.net/11449/73110 |
identifier_str_mv |
Brazilian Dental Journal, v. 22, n. 6, p. 502-510, 2011. 0103-6440 1806-4760 10.1590/S0103-64402011000600011 S0103-64402011000600011 2-s2.0-84255166814 2-s2.0-84255166814.pdf 4517484241515548 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal 0,476 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
502-510 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128545369620480 |