Susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. In western amazonian Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/genes12111693 http://hdl.handle.net/11449/233774 |
Resumo: | Mosquito susceptibility to Plasmodium spp. infection is of paramount importance for malaria occurrence and sustainable transmission. Therefore, understanding the genetic features underlying the mechanisms of susceptibility traits is pivotal to assessing malaria transmission dynamics in endemic areas. The aim of this study was to investigate the susceptibility of Nyssorhynchus darlingi—the dominant malaria vector in Brazil—to Plasmodium spp. using a reduced representation genome-sequencing protocol. The investigation was performed using a genome-wide association study (GWAS) to identify mosquito genes that are predicted to modulate the susceptibility of natural populations of the mosquito to Plasmodium infection. After applying the sequence alignment protocol, we generated the variant panel and filtered variants; leading to the detection of 202,837 SNPs in all specimens analyzed. The resulting panel was used to perform GWAS by comparing the pool of SNP variants present in Ny. darlingi infected with Plasmodium spp. with the pool obtained in field-collected mosquitoes with no evidence of infection by the parasite (all mosquitoes were tested separately using RT-PCR). The GWAS results for infection status showed two statistically significant variants adjacent to important genes that can be associated with susceptibility to Plasmodium infec-tion: Cytochrome P450 (cyp450) and chitinase. This study provides relevant knowledge on malaria transmission dynamics by using a genomic approach to identify mosquito genes associated with susceptibility to Plasmodium infection in Ny. darlingi in western Amazonian Brazil. |
id |
UNSP_83f9fdb7228eb36f30250f9616162971 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/233774 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. In western amazonian BrazilChitinaseCytochrome P450GWASNextRADNyssorhynchus darlingiMosquito susceptibility to Plasmodium spp. infection is of paramount importance for malaria occurrence and sustainable transmission. Therefore, understanding the genetic features underlying the mechanisms of susceptibility traits is pivotal to assessing malaria transmission dynamics in endemic areas. The aim of this study was to investigate the susceptibility of Nyssorhynchus darlingi—the dominant malaria vector in Brazil—to Plasmodium spp. using a reduced representation genome-sequencing protocol. The investigation was performed using a genome-wide association study (GWAS) to identify mosquito genes that are predicted to modulate the susceptibility of natural populations of the mosquito to Plasmodium infection. After applying the sequence alignment protocol, we generated the variant panel and filtered variants; leading to the detection of 202,837 SNPs in all specimens analyzed. The resulting panel was used to perform GWAS by comparing the pool of SNP variants present in Ny. darlingi infected with Plasmodium spp. with the pool obtained in field-collected mosquitoes with no evidence of infection by the parasite (all mosquitoes were tested separately using RT-PCR). The GWAS results for infection status showed two statistically significant variants adjacent to important genes that can be associated with susceptibility to Plasmodium infec-tion: Cytochrome P450 (cyp450) and chitinase. This study provides relevant knowledge on malaria transmission dynamics by using a genomic approach to identify mosquito genes associated with susceptibility to Plasmodium infection in Ny. darlingi in western Amazonian Brazil.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Institutes of HealthConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Biotechnology Institute and Bioscience Institute Sao Paulo State University UNESPDepartamento de Epidemiologia Faculdade de Saúde Pública Universidade de São PauloWadsworth Center New York State Department of HealthDepartment of Biomedical Sciences School of Public Health State University of New YorkBiotechnology Institute and Bioscience Institute Sao Paulo State University UNESPFAPESP: 2014/26229-7National Institutes of Health: 2R01AI110112-06A1CNPq: 301877/2016-5Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)New York State Department of HealthState University of New YorkAlonso, Diego Peres [UNESP]Alvarez, Marcus Vinicius Niz [UNESP]Ribolla, Paulo Eduardo Martins [UNESP]Conn, Jan E.de Oliveira, Tatiane Marques PorangabaSallum, Maria Anice Mureb2022-05-01T10:18:54Z2022-05-01T10:18:54Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/genes12111693Genes, v. 12, n. 11, 2021.2073-4425http://hdl.handle.net/11449/23377410.3390/genes121116932-s2.0-85118474763Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenesinfo:eu-repo/semantics/openAccess2022-05-01T10:18:54Zoai:repositorio.unesp.br:11449/233774Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:11:26.033813Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. In western amazonian Brazil |
title |
Susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. In western amazonian Brazil |
spellingShingle |
Susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. In western amazonian Brazil Alonso, Diego Peres [UNESP] Chitinase Cytochrome P450 GWAS NextRAD Nyssorhynchus darlingi |
title_short |
Susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. In western amazonian Brazil |
title_full |
Susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. In western amazonian Brazil |
title_fullStr |
Susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. In western amazonian Brazil |
title_full_unstemmed |
Susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. In western amazonian Brazil |
title_sort |
Susceptibility of field-collected nyssorhynchus darlingi to plasmodium spp. In western amazonian Brazil |
author |
Alonso, Diego Peres [UNESP] |
author_facet |
Alonso, Diego Peres [UNESP] Alvarez, Marcus Vinicius Niz [UNESP] Ribolla, Paulo Eduardo Martins [UNESP] Conn, Jan E. de Oliveira, Tatiane Marques Porangaba Sallum, Maria Anice Mureb |
author_role |
author |
author2 |
Alvarez, Marcus Vinicius Niz [UNESP] Ribolla, Paulo Eduardo Martins [UNESP] Conn, Jan E. de Oliveira, Tatiane Marques Porangaba Sallum, Maria Anice Mureb |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) New York State Department of Health State University of New York |
dc.contributor.author.fl_str_mv |
Alonso, Diego Peres [UNESP] Alvarez, Marcus Vinicius Niz [UNESP] Ribolla, Paulo Eduardo Martins [UNESP] Conn, Jan E. de Oliveira, Tatiane Marques Porangaba Sallum, Maria Anice Mureb |
dc.subject.por.fl_str_mv |
Chitinase Cytochrome P450 GWAS NextRAD Nyssorhynchus darlingi |
topic |
Chitinase Cytochrome P450 GWAS NextRAD Nyssorhynchus darlingi |
description |
Mosquito susceptibility to Plasmodium spp. infection is of paramount importance for malaria occurrence and sustainable transmission. Therefore, understanding the genetic features underlying the mechanisms of susceptibility traits is pivotal to assessing malaria transmission dynamics in endemic areas. The aim of this study was to investigate the susceptibility of Nyssorhynchus darlingi—the dominant malaria vector in Brazil—to Plasmodium spp. using a reduced representation genome-sequencing protocol. The investigation was performed using a genome-wide association study (GWAS) to identify mosquito genes that are predicted to modulate the susceptibility of natural populations of the mosquito to Plasmodium infection. After applying the sequence alignment protocol, we generated the variant panel and filtered variants; leading to the detection of 202,837 SNPs in all specimens analyzed. The resulting panel was used to perform GWAS by comparing the pool of SNP variants present in Ny. darlingi infected with Plasmodium spp. with the pool obtained in field-collected mosquitoes with no evidence of infection by the parasite (all mosquitoes were tested separately using RT-PCR). The GWAS results for infection status showed two statistically significant variants adjacent to important genes that can be associated with susceptibility to Plasmodium infec-tion: Cytochrome P450 (cyp450) and chitinase. This study provides relevant knowledge on malaria transmission dynamics by using a genomic approach to identify mosquito genes associated with susceptibility to Plasmodium infection in Ny. darlingi in western Amazonian Brazil. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-05-01T10:18:54Z 2022-05-01T10:18:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/genes12111693 Genes, v. 12, n. 11, 2021. 2073-4425 http://hdl.handle.net/11449/233774 10.3390/genes12111693 2-s2.0-85118474763 |
url |
http://dx.doi.org/10.3390/genes12111693 http://hdl.handle.net/11449/233774 |
identifier_str_mv |
Genes, v. 12, n. 11, 2021. 2073-4425 10.3390/genes12111693 2-s2.0-85118474763 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genes |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129169654022144 |