Differential miRNA expression in human macrophage-like cells infected with histoplasma capsulatum yeasts cultured in planktonic and biofilm forms
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/jof7010060 http://hdl.handle.net/11449/208345 |
Resumo: | Histoplasma capsulatum affects healthy and immunocompromised individuals, sometimes causing a severe disease. This fungus has two morphotypes, the mycelial (infective) and the yeast (parasitic) phases. MicroRNAs (miRNAs) are small RNAs involved in the regulation of several cellular processes, and their differential expression has been associated with many disease states. To investigate miRNA expression in host cells during H. capsulatum infection, we studied the changes in the miRNA profiles of differentiated human macrophages infected with yeasts from two fungal strains with different virulence, EH-315 (high virulence) and 60I (low virulence) grown in planktonic cultures, and EH-315 grown in biofilm form. MiRNA profiles were evaluated by means of reverse transcription-quantitative polymerase chain reaction using a commercial human miRNome panel. The target genes of the differentially expressed miRNAs and their corresponding signaling pathways were predicted using bioinformatics analyses. Here, we confirmed biofilm structures were present in the EH-315 culture whose conditions facilitated producing insoluble exopolysaccharide and intracellular polysaccharides. In infected macrophages, bioinformatics analyses revealed especially increased (hsa-miR-99b-3p) or decreased (hsa-miR-342-3p) miRNAs expression levels in response to infection with biofilms or both growth forms of H. capsulatum yeasts, respectively. The results of miRNAs suggested that infection by H. capsulatum can affect important biological pathways of the host cell, targeting two genes: one encoding a protein that is important in the cortical cytoskeleton; the other, a protein involved in the formation of stress granules. Expressed miRNAs in the host’s response could be proposed as new therapeutic and/or diagnostic tools for histoplasmosis. |
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Differential miRNA expression in human macrophage-like cells infected with histoplasma capsulatum yeasts cultured in planktonic and biofilm formsBiofilmsFungal-host interactionsHistoplasma capsulatumMacrophagesMicroRNAsHistoplasma capsulatum affects healthy and immunocompromised individuals, sometimes causing a severe disease. This fungus has two morphotypes, the mycelial (infective) and the yeast (parasitic) phases. MicroRNAs (miRNAs) are small RNAs involved in the regulation of several cellular processes, and their differential expression has been associated with many disease states. To investigate miRNA expression in host cells during H. capsulatum infection, we studied the changes in the miRNA profiles of differentiated human macrophages infected with yeasts from two fungal strains with different virulence, EH-315 (high virulence) and 60I (low virulence) grown in planktonic cultures, and EH-315 grown in biofilm form. MiRNA profiles were evaluated by means of reverse transcription-quantitative polymerase chain reaction using a commercial human miRNome panel. The target genes of the differentially expressed miRNAs and their corresponding signaling pathways were predicted using bioinformatics analyses. Here, we confirmed biofilm structures were present in the EH-315 culture whose conditions facilitated producing insoluble exopolysaccharide and intracellular polysaccharides. In infected macrophages, bioinformatics analyses revealed especially increased (hsa-miR-99b-3p) or decreased (hsa-miR-342-3p) miRNAs expression levels in response to infection with biofilms or both growth forms of H. capsulatum yeasts, respectively. The results of miRNAs suggested that infection by H. capsulatum can affect important biological pathways of the host cell, targeting two genes: one encoding a protein that is important in the cortical cytoskeleton; the other, a protein involved in the formation of stress granules. Expressed miRNAs in the host’s response could be proposed as new therapeutic and/or diagnostic tools for histoplasmosis.Universidade Estadual PaulistaUniversidad Nacional Autónoma de MéxicoConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)School of Pharmaceutical Sciences São Paulo State University (UNESP)Department of Cellular and Molecular Biology Ribeirão Preto School of Medicine University of São PauloSchool of Pharmaceutical Sciences Food and Nutrition Federal University of Mato Grosso do SulUnidad de Micología Departamento de Microbiología y Parasitología Facultad de Medicina UNAM-Universidad Nacional Autónoma de MéxicoDepartamento de Microbiología Escuela Nacional de Ciencias Biológicas IPN–Instituto Politécnico NacionalInstituto de Medicina Molecular Faculdade de Medicina Universidade de LisboaSchool of Pharmaceutical Sciences São Paulo State University (UNESP)Universidad Nacional Autónoma de México: 15090-563-24-V-04CNPq: 480316/2012-0Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Federal University of Mato Grosso do SulUNAM-Universidad Nacional Autónoma de MéxicoIPN–Instituto Politécnico NacionalUniversidade de LisboaPitangui, Nayla de Souza [UNESP]Singulani, Junya de Lacorte [UNESP]Sardi, Janaina de Cássia Orlandide Souza, Paula Carolina [UNESP]Rodríguez-Arellanes, GabrielaGarcía-Pérez, Blanca EstelaEnguita, Francisco JavierPavan, Fernando R. [UNESP]Taylor, Maria LuciaMendes-Giannini, Maria José Soares [UNESP]Fusco-Almeida, Ana Marisa [UNESP]2021-06-25T11:10:35Z2021-06-25T11:10:35Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-23http://dx.doi.org/10.3390/jof7010060Journal of Fungi, v. 7, n. 1, p. 1-23, 2021.2309-608Xhttp://hdl.handle.net/11449/20834510.3390/jof70100602-s2.0-85099828400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Fungiinfo:eu-repo/semantics/openAccess2021-10-23T19:02:07Zoai:repositorio.unesp.br:11449/208345Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:02:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differential miRNA expression in human macrophage-like cells infected with histoplasma capsulatum yeasts cultured in planktonic and biofilm forms |
title |
Differential miRNA expression in human macrophage-like cells infected with histoplasma capsulatum yeasts cultured in planktonic and biofilm forms |
spellingShingle |
Differential miRNA expression in human macrophage-like cells infected with histoplasma capsulatum yeasts cultured in planktonic and biofilm forms Pitangui, Nayla de Souza [UNESP] Biofilms Fungal-host interactions Histoplasma capsulatum Macrophages MicroRNAs |
title_short |
Differential miRNA expression in human macrophage-like cells infected with histoplasma capsulatum yeasts cultured in planktonic and biofilm forms |
title_full |
Differential miRNA expression in human macrophage-like cells infected with histoplasma capsulatum yeasts cultured in planktonic and biofilm forms |
title_fullStr |
Differential miRNA expression in human macrophage-like cells infected with histoplasma capsulatum yeasts cultured in planktonic and biofilm forms |
title_full_unstemmed |
Differential miRNA expression in human macrophage-like cells infected with histoplasma capsulatum yeasts cultured in planktonic and biofilm forms |
title_sort |
Differential miRNA expression in human macrophage-like cells infected with histoplasma capsulatum yeasts cultured in planktonic and biofilm forms |
author |
Pitangui, Nayla de Souza [UNESP] |
author_facet |
Pitangui, Nayla de Souza [UNESP] Singulani, Junya de Lacorte [UNESP] Sardi, Janaina de Cássia Orlandi de Souza, Paula Carolina [UNESP] Rodríguez-Arellanes, Gabriela García-Pérez, Blanca Estela Enguita, Francisco Javier Pavan, Fernando R. [UNESP] Taylor, Maria Lucia Mendes-Giannini, Maria José Soares [UNESP] Fusco-Almeida, Ana Marisa [UNESP] |
author_role |
author |
author2 |
Singulani, Junya de Lacorte [UNESP] Sardi, Janaina de Cássia Orlandi de Souza, Paula Carolina [UNESP] Rodríguez-Arellanes, Gabriela García-Pérez, Blanca Estela Enguita, Francisco Javier Pavan, Fernando R. [UNESP] Taylor, Maria Lucia Mendes-Giannini, Maria José Soares [UNESP] Fusco-Almeida, Ana Marisa [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Federal University of Mato Grosso do Sul UNAM-Universidad Nacional Autónoma de México IPN–Instituto Politécnico Nacional Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Pitangui, Nayla de Souza [UNESP] Singulani, Junya de Lacorte [UNESP] Sardi, Janaina de Cássia Orlandi de Souza, Paula Carolina [UNESP] Rodríguez-Arellanes, Gabriela García-Pérez, Blanca Estela Enguita, Francisco Javier Pavan, Fernando R. [UNESP] Taylor, Maria Lucia Mendes-Giannini, Maria José Soares [UNESP] Fusco-Almeida, Ana Marisa [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms Fungal-host interactions Histoplasma capsulatum Macrophages MicroRNAs |
topic |
Biofilms Fungal-host interactions Histoplasma capsulatum Macrophages MicroRNAs |
description |
Histoplasma capsulatum affects healthy and immunocompromised individuals, sometimes causing a severe disease. This fungus has two morphotypes, the mycelial (infective) and the yeast (parasitic) phases. MicroRNAs (miRNAs) are small RNAs involved in the regulation of several cellular processes, and their differential expression has been associated with many disease states. To investigate miRNA expression in host cells during H. capsulatum infection, we studied the changes in the miRNA profiles of differentiated human macrophages infected with yeasts from two fungal strains with different virulence, EH-315 (high virulence) and 60I (low virulence) grown in planktonic cultures, and EH-315 grown in biofilm form. MiRNA profiles were evaluated by means of reverse transcription-quantitative polymerase chain reaction using a commercial human miRNome panel. The target genes of the differentially expressed miRNAs and their corresponding signaling pathways were predicted using bioinformatics analyses. Here, we confirmed biofilm structures were present in the EH-315 culture whose conditions facilitated producing insoluble exopolysaccharide and intracellular polysaccharides. In infected macrophages, bioinformatics analyses revealed especially increased (hsa-miR-99b-3p) or decreased (hsa-miR-342-3p) miRNAs expression levels in response to infection with biofilms or both growth forms of H. capsulatum yeasts, respectively. The results of miRNAs suggested that infection by H. capsulatum can affect important biological pathways of the host cell, targeting two genes: one encoding a protein that is important in the cortical cytoskeleton; the other, a protein involved in the formation of stress granules. Expressed miRNAs in the host’s response could be proposed as new therapeutic and/or diagnostic tools for histoplasmosis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:10:35Z 2021-06-25T11:10:35Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/jof7010060 Journal of Fungi, v. 7, n. 1, p. 1-23, 2021. 2309-608X http://hdl.handle.net/11449/208345 10.3390/jof7010060 2-s2.0-85099828400 |
url |
http://dx.doi.org/10.3390/jof7010060 http://hdl.handle.net/11449/208345 |
identifier_str_mv |
Journal of Fungi, v. 7, n. 1, p. 1-23, 2021. 2309-608X 10.3390/jof7010060 2-s2.0-85099828400 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Fungi |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-23 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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_version_ |
1799965324342198272 |