Genetic Studies on the Effects of Nanomaterials
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/978-1-4614-8993-1_8 http://hdl.handle.net/11449/245687 |
Resumo: | The aim of this chapter is to present some of the principal methodologies used to study the effects of toxic substances on DNA, which can be used to analyze aspects of nanomaterial toxicity. It is not the intention here to undertake an in-depth survey of the topic, but rather to highlight the techniques that can be used in nanotoxicity studies. The available methods for evaluating effects on DNA include genotoxicity tests such as (1) the Allium cepa chromosome aberration test, (2) the comet analysis, (3) the micronucleus test, and (4) the cytogenetic analysis. In addition, this chapter also described the methodologies for analysis of gene expression that can be applied to the effects of nanomaterials. A description of the characteristics of each method is provided, together with results of selected studies that have evaluated the effects of nanomaterials, and a critical discussion of the main advantages and disadvantages of each technique. The chapter also highlights the challenges and future perspectives for studies of the effects of nanostructured materials on DNA. |
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Genetic Studies on the Effects of NanomaterialsThe aim of this chapter is to present some of the principal methodologies used to study the effects of toxic substances on DNA, which can be used to analyze aspects of nanomaterial toxicity. It is not the intention here to undertake an in-depth survey of the topic, but rather to highlight the techniques that can be used in nanotoxicity studies. The available methods for evaluating effects on DNA include genotoxicity tests such as (1) the Allium cepa chromosome aberration test, (2) the comet analysis, (3) the micronucleus test, and (4) the cytogenetic analysis. In addition, this chapter also described the methodologies for analysis of gene expression that can be applied to the effects of nanomaterials. A description of the characteristics of each method is provided, together with results of selected studies that have evaluated the effects of nanomaterials, and a critical discussion of the main advantages and disadvantages of each technique. The chapter also highlights the challenges and future perspectives for studies of the effects of nanostructured materials on DNA.Univ Sorocaba, Dept Biotechnol, BR-18023000 Sao Paulo, BrazilUniv Fed Sao Carlos, Sao Paulo, BrazilSao Paulo State Univ, UNESP, Dept Environm Engn, BR-18087180 Sao Paulo, BrazilSao Paulo State Univ, UNESP, Dept Environm Engn, BR-18087180 Sao Paulo, BrazilSpringerUniv SorocabaUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)Lima, Renata deFraceto, Leonardo Fernandes [UNESP]Duran, N.Guterres, S. S.Alves, O. L.2023-07-29T12:02:06Z2023-07-29T12:02:06Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article177-199http://dx.doi.org/10.1007/978-1-4614-8993-1_8Nanotoxicology: Materials, Methodologies, and Assessments. Berlin: Springer-verlag Berlin, p. 177-199, 2014.2194-0452http://hdl.handle.net/11449/24568710.1007/978-1-4614-8993-1_8WOS:000332364400010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNanotoxicology: Materials, Methodologies, And Assessmentsinfo:eu-repo/semantics/openAccess2023-07-29T12:02:06Zoai:repositorio.unesp.br:11449/245687Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:44:16.478255Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic Studies on the Effects of Nanomaterials |
title |
Genetic Studies on the Effects of Nanomaterials |
spellingShingle |
Genetic Studies on the Effects of Nanomaterials Lima, Renata de |
title_short |
Genetic Studies on the Effects of Nanomaterials |
title_full |
Genetic Studies on the Effects of Nanomaterials |
title_fullStr |
Genetic Studies on the Effects of Nanomaterials |
title_full_unstemmed |
Genetic Studies on the Effects of Nanomaterials |
title_sort |
Genetic Studies on the Effects of Nanomaterials |
author |
Lima, Renata de |
author_facet |
Lima, Renata de Fraceto, Leonardo Fernandes [UNESP] Duran, N. Guterres, S. S. Alves, O. L. |
author_role |
author |
author2 |
Fraceto, Leonardo Fernandes [UNESP] Duran, N. Guterres, S. S. Alves, O. L. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Univ Sorocaba Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Lima, Renata de Fraceto, Leonardo Fernandes [UNESP] Duran, N. Guterres, S. S. Alves, O. L. |
description |
The aim of this chapter is to present some of the principal methodologies used to study the effects of toxic substances on DNA, which can be used to analyze aspects of nanomaterial toxicity. It is not the intention here to undertake an in-depth survey of the topic, but rather to highlight the techniques that can be used in nanotoxicity studies. The available methods for evaluating effects on DNA include genotoxicity tests such as (1) the Allium cepa chromosome aberration test, (2) the comet analysis, (3) the micronucleus test, and (4) the cytogenetic analysis. In addition, this chapter also described the methodologies for analysis of gene expression that can be applied to the effects of nanomaterials. A description of the characteristics of each method is provided, together with results of selected studies that have evaluated the effects of nanomaterials, and a critical discussion of the main advantages and disadvantages of each technique. The chapter also highlights the challenges and future perspectives for studies of the effects of nanostructured materials on DNA. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 2023-07-29T12:02:06Z 2023-07-29T12:02:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/978-1-4614-8993-1_8 Nanotoxicology: Materials, Methodologies, and Assessments. Berlin: Springer-verlag Berlin, p. 177-199, 2014. 2194-0452 http://hdl.handle.net/11449/245687 10.1007/978-1-4614-8993-1_8 WOS:000332364400010 |
url |
http://dx.doi.org/10.1007/978-1-4614-8993-1_8 http://hdl.handle.net/11449/245687 |
identifier_str_mv |
Nanotoxicology: Materials, Methodologies, and Assessments. Berlin: Springer-verlag Berlin, p. 177-199, 2014. 2194-0452 10.1007/978-1-4614-8993-1_8 WOS:000332364400010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nanotoxicology: Materials, Methodologies, And Assessments |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
177-199 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129456767762432 |