Gastrointestinal motility and morphology in mice: Strain-dependent differences
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/nmo.13824 http://hdl.handle.net/11449/201770 |
Resumo: | Background: BALB/c and C57BL/6 mice are widely used in biomedical research; however, the differences between strains are still underestimated. Our aims were to develop an experimental protocol to evaluate the duodenal contractility and gastrointestinal transit in mice using the Alternating Current Biosusceptometry (ACB) technique and to compare gastrointestinal motor function and morphology between BALB/c and C57BL/6 strains. Methods: Male mice were used in experiments (a) duodenal contractility: animals which had a magnetic marker surgically fixed in the duodenum to determine the frequency and amplitude of contractions and (b) gastrointestinal transit: animals which ingested a magnetically marked chow to calculate the Oro-Anal Transit Time (OATT) and the Fecal Pellet Elimination Rate (FPER). The animals were killed after the experiments for organ collection and morphometric analysis. Key Results: BALB/c and C57BL/6 had two different duodenal frequencies (high and low) with similar amplitudes. After 10 hours of monitoring, BALB/c eliminated around 89% of the ingested marker and C57BL/6 eliminated 33%; OATT and FPER were slower for C57BL/6 compared with BALB/c. The OATT and amplitude of low frequency had a strong positive correlation in C57BL/6. For BALB/c, the gastric muscular layer was thicker compared to that measured for C57BL/6. Conclusions and Inferences: The experimental protocol to evaluate duodenal contractility and fecal magnetic pellets output using the ACB technique in mice was successfully established. BALB/c strains had higher duodenal frequencies and a shorter time to eliminate the ingested marker. Our results showed differences in both motor function and gastrointestinal morphology between BALB/c and C57BL/6 strains. |
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Repositório Institucional da UNESP |
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Gastrointestinal motility and morphology in mice: Strain-dependent differencesBALB/cC57BL/6duodenal contractilitygastrointestinal transitinbredBackground: BALB/c and C57BL/6 mice are widely used in biomedical research; however, the differences between strains are still underestimated. Our aims were to develop an experimental protocol to evaluate the duodenal contractility and gastrointestinal transit in mice using the Alternating Current Biosusceptometry (ACB) technique and to compare gastrointestinal motor function and morphology between BALB/c and C57BL/6 strains. Methods: Male mice were used in experiments (a) duodenal contractility: animals which had a magnetic marker surgically fixed in the duodenum to determine the frequency and amplitude of contractions and (b) gastrointestinal transit: animals which ingested a magnetically marked chow to calculate the Oro-Anal Transit Time (OATT) and the Fecal Pellet Elimination Rate (FPER). The animals were killed after the experiments for organ collection and morphometric analysis. Key Results: BALB/c and C57BL/6 had two different duodenal frequencies (high and low) with similar amplitudes. After 10 hours of monitoring, BALB/c eliminated around 89% of the ingested marker and C57BL/6 eliminated 33%; OATT and FPER were slower for C57BL/6 compared with BALB/c. The OATT and amplitude of low frequency had a strong positive correlation in C57BL/6. For BALB/c, the gastric muscular layer was thicker compared to that measured for C57BL/6. Conclusions and Inferences: The experimental protocol to evaluate duodenal contractility and fecal magnetic pellets output using the ACB technique in mice was successfully established. BALB/c strains had higher duodenal frequencies and a shorter time to eliminate the ingested marker. Our results showed differences in both motor function and gastrointestinal morphology between BALB/c and C57BL/6 strains.Institute of Biosciences São Paulo State University UNESPInstitute of Biological Sciences and Health Federal University of Mato Grosso UFMTAlagoas State University of Health Sciences UNCISALInstitute of Biosciences São Paulo State University UNESPUniversidade Estadual Paulista (Unesp)UFMTUNCISALGama, Loyane Almeida [UNESP]Rocha Machado, Mariana Pirani [UNESP]Beckmann, Ana Paula SimõesMiranda, José Ricardo de Arruda [UNESP]Corá, Luciana AparecidaAmérico, Madileine Francely [UNESP]2020-12-12T02:41:23Z2020-12-12T02:41:23Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/nmo.13824Neurogastroenterology and Motility, v. 32, n. 6, 2020.1365-29821350-1925http://hdl.handle.net/11449/20177010.1111/nmo.138242-s2.0-85084924419Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeurogastroenterology and Motilityinfo:eu-repo/semantics/openAccess2021-10-22T21:54:16Zoai:repositorio.unesp.br:11449/201770Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T20:47:29.020016Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gastrointestinal motility and morphology in mice: Strain-dependent differences |
title |
Gastrointestinal motility and morphology in mice: Strain-dependent differences |
spellingShingle |
Gastrointestinal motility and morphology in mice: Strain-dependent differences Gama, Loyane Almeida [UNESP] BALB/c C57BL/6 duodenal contractility gastrointestinal transit inbred |
title_short |
Gastrointestinal motility and morphology in mice: Strain-dependent differences |
title_full |
Gastrointestinal motility and morphology in mice: Strain-dependent differences |
title_fullStr |
Gastrointestinal motility and morphology in mice: Strain-dependent differences |
title_full_unstemmed |
Gastrointestinal motility and morphology in mice: Strain-dependent differences |
title_sort |
Gastrointestinal motility and morphology in mice: Strain-dependent differences |
author |
Gama, Loyane Almeida [UNESP] |
author_facet |
Gama, Loyane Almeida [UNESP] Rocha Machado, Mariana Pirani [UNESP] Beckmann, Ana Paula Simões Miranda, José Ricardo de Arruda [UNESP] Corá, Luciana Aparecida Américo, Madileine Francely [UNESP] |
author_role |
author |
author2 |
Rocha Machado, Mariana Pirani [UNESP] Beckmann, Ana Paula Simões Miranda, José Ricardo de Arruda [UNESP] Corá, Luciana Aparecida Américo, Madileine Francely [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UFMT UNCISAL |
dc.contributor.author.fl_str_mv |
Gama, Loyane Almeida [UNESP] Rocha Machado, Mariana Pirani [UNESP] Beckmann, Ana Paula Simões Miranda, José Ricardo de Arruda [UNESP] Corá, Luciana Aparecida Américo, Madileine Francely [UNESP] |
dc.subject.por.fl_str_mv |
BALB/c C57BL/6 duodenal contractility gastrointestinal transit inbred |
topic |
BALB/c C57BL/6 duodenal contractility gastrointestinal transit inbred |
description |
Background: BALB/c and C57BL/6 mice are widely used in biomedical research; however, the differences between strains are still underestimated. Our aims were to develop an experimental protocol to evaluate the duodenal contractility and gastrointestinal transit in mice using the Alternating Current Biosusceptometry (ACB) technique and to compare gastrointestinal motor function and morphology between BALB/c and C57BL/6 strains. Methods: Male mice were used in experiments (a) duodenal contractility: animals which had a magnetic marker surgically fixed in the duodenum to determine the frequency and amplitude of contractions and (b) gastrointestinal transit: animals which ingested a magnetically marked chow to calculate the Oro-Anal Transit Time (OATT) and the Fecal Pellet Elimination Rate (FPER). The animals were killed after the experiments for organ collection and morphometric analysis. Key Results: BALB/c and C57BL/6 had two different duodenal frequencies (high and low) with similar amplitudes. After 10 hours of monitoring, BALB/c eliminated around 89% of the ingested marker and C57BL/6 eliminated 33%; OATT and FPER were slower for C57BL/6 compared with BALB/c. The OATT and amplitude of low frequency had a strong positive correlation in C57BL/6. For BALB/c, the gastric muscular layer was thicker compared to that measured for C57BL/6. Conclusions and Inferences: The experimental protocol to evaluate duodenal contractility and fecal magnetic pellets output using the ACB technique in mice was successfully established. BALB/c strains had higher duodenal frequencies and a shorter time to eliminate the ingested marker. Our results showed differences in both motor function and gastrointestinal morphology between BALB/c and C57BL/6 strains. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:41:23Z 2020-12-12T02:41:23Z 2020-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/nmo.13824 Neurogastroenterology and Motility, v. 32, n. 6, 2020. 1365-2982 1350-1925 http://hdl.handle.net/11449/201770 10.1111/nmo.13824 2-s2.0-85084924419 |
url |
http://dx.doi.org/10.1111/nmo.13824 http://hdl.handle.net/11449/201770 |
identifier_str_mv |
Neurogastroenterology and Motility, v. 32, n. 6, 2020. 1365-2982 1350-1925 10.1111/nmo.13824 2-s2.0-85084924419 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neurogastroenterology and Motility |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045694981799936 |