Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/nano12203547 http://hdl.handle.net/11449/249318 |
Resumo: | Photodynamic therapy (PDT) mediated by photosensitizers loaded in nanostructures as solid lipid nanoparticles has been pinpointed as an effective and safe treatment against different skin cancers. Amazon butters have an interesting lipid composition when it comes to forming solid lipid nanoparticles (SLN). In the present report, a new third-generation photosensitizing system consisting of aluminum-phthalocyanine associated with Amazon butter-based solid lipid nanoparticles (SLN-AlPc) is described. The SLN was developed using murumuru butter, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 40 nm was obtained. The study of the permeation of these AlPc did not permeate the analyzed skin, but when incorporated into the system, SLN-AlPc allowed permeation of almost 100% with 8 h of contact. It must be emphasized that SLN-AlPc was efficient for carrying aluminum-phthalocyanine photosensitizers and exhibited no toxicity in the dark. Photoactivated SLN-AlPc exhibited a 50% cytotoxicity concentration (IC50) of 19.62 nM when applied to B16-F10 monolayers, and the type of death caused by the treatment was apoptosis. The exposed phospholipid phosphatidylserine was identified, and the treatment triggered a high expression of Caspase 3. A stable Amazon butter-based SLN-AlPc formulation was developed, which exhibits strong in vitro photodynamic activity on melanoma cells. |
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Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against MelanomaAmazon butterscancerdrug delivery systemsphotodynamic therapythird-generation photosensitizersPhotodynamic therapy (PDT) mediated by photosensitizers loaded in nanostructures as solid lipid nanoparticles has been pinpointed as an effective and safe treatment against different skin cancers. Amazon butters have an interesting lipid composition when it comes to forming solid lipid nanoparticles (SLN). In the present report, a new third-generation photosensitizing system consisting of aluminum-phthalocyanine associated with Amazon butter-based solid lipid nanoparticles (SLN-AlPc) is described. The SLN was developed using murumuru butter, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 40 nm was obtained. The study of the permeation of these AlPc did not permeate the analyzed skin, but when incorporated into the system, SLN-AlPc allowed permeation of almost 100% with 8 h of contact. It must be emphasized that SLN-AlPc was efficient for carrying aluminum-phthalocyanine photosensitizers and exhibited no toxicity in the dark. Photoactivated SLN-AlPc exhibited a 50% cytotoxicity concentration (IC50) of 19.62 nM when applied to B16-F10 monolayers, and the type of death caused by the treatment was apoptosis. The exposed phospholipid phosphatidylserine was identified, and the treatment triggered a high expression of Caspase 3. A stable Amazon butter-based SLN-AlPc formulation was developed, which exhibits strong in vitro photodynamic activity on melanoma cells.Fundação de Apoio à Pesquisa do Distrito FederalConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Postgraduate Program in Animal Biology Department of Genetics and Morphology Institute of Biological Sciences University of Brasília, DFLaboratory of Microscopy and Microanalysis Department of Cell Biology Institute of Biological Sciences University of Brasília, DFCOOIL Institute, DFSchool of Pharmaceutical Sciences São Paulo State University, SPLaboratory of Nanobiotechnology Department of Genetics and Morphology Institute of Biological Sciences University of Brasilia, DFLaboratory of Immunology and Inflammation Department of Cell Biology University of Brasilia, DFOptical Spectroscopy Laboratory Institute of Physics University of Brasilia, DFLaboratory of Protein Chemistry and Biochemistry Department of Cell Biology Institute of Biology University of Brasília, DFFaculty of Ceilandia University of Brasilia, DFLaboratory of Electron Paramagnetic Resonance Institute of Physics University of Brasília, DFSchool of Pharmaceutical Sciences São Paulo State University, SPFundação de Apoio à Pesquisa do Distrito Federal: 00193.00000920/2020-23Fundação de Apoio à Pesquisa do Distrito Federal: 00193.00001053/2021-24CNPq: 302355/2016-2CNPq: 401957/2016-0CNPq: 40356/2021University of BrasíliaCOOIL InstituteUniversidade Estadual Paulista (UNESP)University of BrasiliaMello, Victor CarlosAraújo, Victor Hugo Sousa [UNESP]de Paiva, Karen Letycia RodriguesSimões, Marina MesquitaMarques, Dafne Carolineda Silva Costa, Nelice Robertade Souza, Isadora Florêncioda Silva, Patricia BentoSantos, IgorAlmeida, RaquelMagalhães, Kelly Graceda Silva, Sebastião WilliamSantos, Alexandre SilvaVeiga-Souza, FabianeSouza, Paulo Eduardo NarcizoRaddichi, Marina ArantesLongo, João Paulo Figueiróde Araújo, Jennifer Thayanne Cavalcante [UNESP]Muehlmann, Luis AlexandreChorilli, Marlus [UNESP]Báo, Sônia Nair2023-07-29T15:12:46Z2023-07-29T15:12:46Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/nano12203547Nanomaterials, v. 12, n. 20, 2022.2079-4991http://hdl.handle.net/11449/24931810.3390/nano122035472-s2.0-85140910464Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNanomaterialsinfo:eu-repo/semantics/openAccess2024-06-24T13:46:32Zoai:repositorio.unesp.br:11449/249318Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:00:47.539370Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma |
title |
Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma |
spellingShingle |
Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma Mello, Victor Carlos Amazon butters cancer drug delivery systems photodynamic therapy third-generation photosensitizers |
title_short |
Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma |
title_full |
Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma |
title_fullStr |
Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma |
title_full_unstemmed |
Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma |
title_sort |
Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma |
author |
Mello, Victor Carlos |
author_facet |
Mello, Victor Carlos Araújo, Victor Hugo Sousa [UNESP] de Paiva, Karen Letycia Rodrigues Simões, Marina Mesquita Marques, Dafne Caroline da Silva Costa, Nelice Roberta de Souza, Isadora Florêncio da Silva, Patricia Bento Santos, Igor Almeida, Raquel Magalhães, Kelly Grace da Silva, Sebastião William Santos, Alexandre Silva Veiga-Souza, Fabiane Souza, Paulo Eduardo Narcizo Raddichi, Marina Arantes Longo, João Paulo Figueiró de Araújo, Jennifer Thayanne Cavalcante [UNESP] Muehlmann, Luis Alexandre Chorilli, Marlus [UNESP] Báo, Sônia Nair |
author_role |
author |
author2 |
Araújo, Victor Hugo Sousa [UNESP] de Paiva, Karen Letycia Rodrigues Simões, Marina Mesquita Marques, Dafne Caroline da Silva Costa, Nelice Roberta de Souza, Isadora Florêncio da Silva, Patricia Bento Santos, Igor Almeida, Raquel Magalhães, Kelly Grace da Silva, Sebastião William Santos, Alexandre Silva Veiga-Souza, Fabiane Souza, Paulo Eduardo Narcizo Raddichi, Marina Arantes Longo, João Paulo Figueiró de Araújo, Jennifer Thayanne Cavalcante [UNESP] Muehlmann, Luis Alexandre Chorilli, Marlus [UNESP] Báo, Sônia Nair |
author2_role |
author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Brasília COOIL Institute Universidade Estadual Paulista (UNESP) University of Brasilia |
dc.contributor.author.fl_str_mv |
Mello, Victor Carlos Araújo, Victor Hugo Sousa [UNESP] de Paiva, Karen Letycia Rodrigues Simões, Marina Mesquita Marques, Dafne Caroline da Silva Costa, Nelice Roberta de Souza, Isadora Florêncio da Silva, Patricia Bento Santos, Igor Almeida, Raquel Magalhães, Kelly Grace da Silva, Sebastião William Santos, Alexandre Silva Veiga-Souza, Fabiane Souza, Paulo Eduardo Narcizo Raddichi, Marina Arantes Longo, João Paulo Figueiró de Araújo, Jennifer Thayanne Cavalcante [UNESP] Muehlmann, Luis Alexandre Chorilli, Marlus [UNESP] Báo, Sônia Nair |
dc.subject.por.fl_str_mv |
Amazon butters cancer drug delivery systems photodynamic therapy third-generation photosensitizers |
topic |
Amazon butters cancer drug delivery systems photodynamic therapy third-generation photosensitizers |
description |
Photodynamic therapy (PDT) mediated by photosensitizers loaded in nanostructures as solid lipid nanoparticles has been pinpointed as an effective and safe treatment against different skin cancers. Amazon butters have an interesting lipid composition when it comes to forming solid lipid nanoparticles (SLN). In the present report, a new third-generation photosensitizing system consisting of aluminum-phthalocyanine associated with Amazon butter-based solid lipid nanoparticles (SLN-AlPc) is described. The SLN was developed using murumuru butter, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 40 nm was obtained. The study of the permeation of these AlPc did not permeate the analyzed skin, but when incorporated into the system, SLN-AlPc allowed permeation of almost 100% with 8 h of contact. It must be emphasized that SLN-AlPc was efficient for carrying aluminum-phthalocyanine photosensitizers and exhibited no toxicity in the dark. Photoactivated SLN-AlPc exhibited a 50% cytotoxicity concentration (IC50) of 19.62 nM when applied to B16-F10 monolayers, and the type of death caused by the treatment was apoptosis. The exposed phospholipid phosphatidylserine was identified, and the treatment triggered a high expression of Caspase 3. A stable Amazon butter-based SLN-AlPc formulation was developed, which exhibits strong in vitro photodynamic activity on melanoma cells. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-01 2023-07-29T15:12:46Z 2023-07-29T15:12:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/nano12203547 Nanomaterials, v. 12, n. 20, 2022. 2079-4991 http://hdl.handle.net/11449/249318 10.3390/nano12203547 2-s2.0-85140910464 |
url |
http://dx.doi.org/10.3390/nano12203547 http://hdl.handle.net/11449/249318 |
identifier_str_mv |
Nanomaterials, v. 12, n. 20, 2022. 2079-4991 10.3390/nano12203547 2-s2.0-85140910464 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nanomaterials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128239113076736 |