Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma

Detalhes bibliográficos
Autor(a) principal: Mello, Victor Carlos
Data de Publicação: 2022
Outros Autores: Araújo, Victor Hugo Sousa [UNESP], de Paiva, Karen Letycia Rodrigues, Simões, Marina Mesquita, Marques, Dafne Caroline, da Silva Costa, Nelice Roberta, de Souza, Isadora Florêncio, da Silva, Patricia Bento, Santos, Igor, Almeida, Raquel, Magalhães, Kelly Grace, da Silva, Sebastião William, Santos, Alexandre Silva, Veiga-Souza, Fabiane, Souza, Paulo Eduardo Narcizo, Raddichi, Marina Arantes, Longo, João Paulo Figueiró, de Araújo, Jennifer Thayanne Cavalcante [UNESP], Muehlmann, Luis Alexandre, Chorilli, Marlus [UNESP], Báo, Sônia Nair
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/nano12203547
http://hdl.handle.net/11449/249318
Resumo: Photodynamic therapy (PDT) mediated by photosensitizers loaded in nanostructures as solid lipid nanoparticles has been pinpointed as an effective and safe treatment against different skin cancers. Amazon butters have an interesting lipid composition when it comes to forming solid lipid nanoparticles (SLN). In the present report, a new third-generation photosensitizing system consisting of aluminum-phthalocyanine associated with Amazon butter-based solid lipid nanoparticles (SLN-AlPc) is described. The SLN was developed using murumuru butter, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 40 nm was obtained. The study of the permeation of these AlPc did not permeate the analyzed skin, but when incorporated into the system, SLN-AlPc allowed permeation of almost 100% with 8 h of contact. It must be emphasized that SLN-AlPc was efficient for carrying aluminum-phthalocyanine photosensitizers and exhibited no toxicity in the dark. Photoactivated SLN-AlPc exhibited a 50% cytotoxicity concentration (IC50) of 19.62 nM when applied to B16-F10 monolayers, and the type of death caused by the treatment was apoptosis. The exposed phospholipid phosphatidylserine was identified, and the treatment triggered a high expression of Caspase 3. A stable Amazon butter-based SLN-AlPc formulation was developed, which exhibits strong in vitro photodynamic activity on melanoma cells.
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spelling Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against MelanomaAmazon butterscancerdrug delivery systemsphotodynamic therapythird-generation photosensitizersPhotodynamic therapy (PDT) mediated by photosensitizers loaded in nanostructures as solid lipid nanoparticles has been pinpointed as an effective and safe treatment against different skin cancers. Amazon butters have an interesting lipid composition when it comes to forming solid lipid nanoparticles (SLN). In the present report, a new third-generation photosensitizing system consisting of aluminum-phthalocyanine associated with Amazon butter-based solid lipid nanoparticles (SLN-AlPc) is described. The SLN was developed using murumuru butter, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 40 nm was obtained. The study of the permeation of these AlPc did not permeate the analyzed skin, but when incorporated into the system, SLN-AlPc allowed permeation of almost 100% with 8 h of contact. It must be emphasized that SLN-AlPc was efficient for carrying aluminum-phthalocyanine photosensitizers and exhibited no toxicity in the dark. Photoactivated SLN-AlPc exhibited a 50% cytotoxicity concentration (IC50) of 19.62 nM when applied to B16-F10 monolayers, and the type of death caused by the treatment was apoptosis. The exposed phospholipid phosphatidylserine was identified, and the treatment triggered a high expression of Caspase 3. A stable Amazon butter-based SLN-AlPc formulation was developed, which exhibits strong in vitro photodynamic activity on melanoma cells.Fundação de Apoio à Pesquisa do Distrito FederalConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Postgraduate Program in Animal Biology Department of Genetics and Morphology Institute of Biological Sciences University of Brasília, DFLaboratory of Microscopy and Microanalysis Department of Cell Biology Institute of Biological Sciences University of Brasília, DFCOOIL Institute, DFSchool of Pharmaceutical Sciences São Paulo State University, SPLaboratory of Nanobiotechnology Department of Genetics and Morphology Institute of Biological Sciences University of Brasilia, DFLaboratory of Immunology and Inflammation Department of Cell Biology University of Brasilia, DFOptical Spectroscopy Laboratory Institute of Physics University of Brasilia, DFLaboratory of Protein Chemistry and Biochemistry Department of Cell Biology Institute of Biology University of Brasília, DFFaculty of Ceilandia University of Brasilia, DFLaboratory of Electron Paramagnetic Resonance Institute of Physics University of Brasília, DFSchool of Pharmaceutical Sciences São Paulo State University, SPFundação de Apoio à Pesquisa do Distrito Federal: 00193.00000920/2020-23Fundação de Apoio à Pesquisa do Distrito Federal: 00193.00001053/2021-24CNPq: 302355/2016-2CNPq: 401957/2016-0CNPq: 40356/2021University of BrasíliaCOOIL InstituteUniversidade Estadual Paulista (UNESP)University of BrasiliaMello, Victor CarlosAraújo, Victor Hugo Sousa [UNESP]de Paiva, Karen Letycia RodriguesSimões, Marina MesquitaMarques, Dafne Carolineda Silva Costa, Nelice Robertade Souza, Isadora Florêncioda Silva, Patricia BentoSantos, IgorAlmeida, RaquelMagalhães, Kelly Graceda Silva, Sebastião WilliamSantos, Alexandre SilvaVeiga-Souza, FabianeSouza, Paulo Eduardo NarcizoRaddichi, Marina ArantesLongo, João Paulo Figueiróde Araújo, Jennifer Thayanne Cavalcante [UNESP]Muehlmann, Luis AlexandreChorilli, Marlus [UNESP]Báo, Sônia Nair2023-07-29T15:12:46Z2023-07-29T15:12:46Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/nano12203547Nanomaterials, v. 12, n. 20, 2022.2079-4991http://hdl.handle.net/11449/24931810.3390/nano122035472-s2.0-85140910464Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNanomaterialsinfo:eu-repo/semantics/openAccess2024-06-24T13:46:32Zoai:repositorio.unesp.br:11449/249318Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:00:47.539370Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma
title Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma
spellingShingle Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma
Mello, Victor Carlos
Amazon butters
cancer
drug delivery systems
photodynamic therapy
third-generation photosensitizers
title_short Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma
title_full Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma
title_fullStr Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma
title_full_unstemmed Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma
title_sort Development of New Natural Lipid-Based Nanoparticles Loaded with Aluminum-Phthalocyanine for Photodynamic Therapy against Melanoma
author Mello, Victor Carlos
author_facet Mello, Victor Carlos
Araújo, Victor Hugo Sousa [UNESP]
de Paiva, Karen Letycia Rodrigues
Simões, Marina Mesquita
Marques, Dafne Caroline
da Silva Costa, Nelice Roberta
de Souza, Isadora Florêncio
da Silva, Patricia Bento
Santos, Igor
Almeida, Raquel
Magalhães, Kelly Grace
da Silva, Sebastião William
Santos, Alexandre Silva
Veiga-Souza, Fabiane
Souza, Paulo Eduardo Narcizo
Raddichi, Marina Arantes
Longo, João Paulo Figueiró
de Araújo, Jennifer Thayanne Cavalcante [UNESP]
Muehlmann, Luis Alexandre
Chorilli, Marlus [UNESP]
Báo, Sônia Nair
author_role author
author2 Araújo, Victor Hugo Sousa [UNESP]
de Paiva, Karen Letycia Rodrigues
Simões, Marina Mesquita
Marques, Dafne Caroline
da Silva Costa, Nelice Roberta
de Souza, Isadora Florêncio
da Silva, Patricia Bento
Santos, Igor
Almeida, Raquel
Magalhães, Kelly Grace
da Silva, Sebastião William
Santos, Alexandre Silva
Veiga-Souza, Fabiane
Souza, Paulo Eduardo Narcizo
Raddichi, Marina Arantes
Longo, João Paulo Figueiró
de Araújo, Jennifer Thayanne Cavalcante [UNESP]
Muehlmann, Luis Alexandre
Chorilli, Marlus [UNESP]
Báo, Sônia Nair
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Brasília
COOIL Institute
Universidade Estadual Paulista (UNESP)
University of Brasilia
dc.contributor.author.fl_str_mv Mello, Victor Carlos
Araújo, Victor Hugo Sousa [UNESP]
de Paiva, Karen Letycia Rodrigues
Simões, Marina Mesquita
Marques, Dafne Caroline
da Silva Costa, Nelice Roberta
de Souza, Isadora Florêncio
da Silva, Patricia Bento
Santos, Igor
Almeida, Raquel
Magalhães, Kelly Grace
da Silva, Sebastião William
Santos, Alexandre Silva
Veiga-Souza, Fabiane
Souza, Paulo Eduardo Narcizo
Raddichi, Marina Arantes
Longo, João Paulo Figueiró
de Araújo, Jennifer Thayanne Cavalcante [UNESP]
Muehlmann, Luis Alexandre
Chorilli, Marlus [UNESP]
Báo, Sônia Nair
dc.subject.por.fl_str_mv Amazon butters
cancer
drug delivery systems
photodynamic therapy
third-generation photosensitizers
topic Amazon butters
cancer
drug delivery systems
photodynamic therapy
third-generation photosensitizers
description Photodynamic therapy (PDT) mediated by photosensitizers loaded in nanostructures as solid lipid nanoparticles has been pinpointed as an effective and safe treatment against different skin cancers. Amazon butters have an interesting lipid composition when it comes to forming solid lipid nanoparticles (SLN). In the present report, a new third-generation photosensitizing system consisting of aluminum-phthalocyanine associated with Amazon butter-based solid lipid nanoparticles (SLN-AlPc) is described. The SLN was developed using murumuru butter, and a monodisperse population of nanodroplets with a hydrodynamic diameter of approximately 40 nm was obtained. The study of the permeation of these AlPc did not permeate the analyzed skin, but when incorporated into the system, SLN-AlPc allowed permeation of almost 100% with 8 h of contact. It must be emphasized that SLN-AlPc was efficient for carrying aluminum-phthalocyanine photosensitizers and exhibited no toxicity in the dark. Photoactivated SLN-AlPc exhibited a 50% cytotoxicity concentration (IC50) of 19.62 nM when applied to B16-F10 monolayers, and the type of death caused by the treatment was apoptosis. The exposed phospholipid phosphatidylserine was identified, and the treatment triggered a high expression of Caspase 3. A stable Amazon butter-based SLN-AlPc formulation was developed, which exhibits strong in vitro photodynamic activity on melanoma cells.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-01
2023-07-29T15:12:46Z
2023-07-29T15:12:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/nano12203547
Nanomaterials, v. 12, n. 20, 2022.
2079-4991
http://hdl.handle.net/11449/249318
10.3390/nano12203547
2-s2.0-85140910464
url http://dx.doi.org/10.3390/nano12203547
http://hdl.handle.net/11449/249318
identifier_str_mv Nanomaterials, v. 12, n. 20, 2022.
2079-4991
10.3390/nano12203547
2-s2.0-85140910464
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nanomaterials
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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