Cross-reactivity between antigens from Amblyomma cajennense and A. hebraeum (Acari: Ixodidae)
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4102/jsava.v75i1.447 http://jsava.co.za/index.php/jsava/article/view/447 http://hdl.handle.net/11449/243624 |
Resumo: | Laboratory animals exposed to feeding ticks develop resistance which is reflected by a decline in tick engorgement weight, egg-laying by adults and reduced egg viability. Serum antibodies from these hosts and their reaction with tick antigens have been detected by different methods, including precipitation techniques, immunofluorescent techniques, ELISA and Western blots. However, little is known about the effects of antibodies on ticks that engorge on resistant hosts, or which tissues of the tick body are possibly immunogenic. Some researchers, using immunohistochemistry, have detected host antibodies in the gut, salivary glands and haemolymph of ticks engorged on resistant animals. The same technique has helped considerably in determining antigenic sites or antibody target in other arthropods. Consequently, immunohistochemistry techniques were used in this study to detect cross-reactivity between sera raised against Amblyomma cajennense (Fabricius, 1787) with Amblyomma hebraeum (Koch, 1844), and vice versa. The results show the existence of shared antigens between the 2 tick species. In general, our results point more to a 1-way cross-reactivity of A. hebraeum with A. cajennense than a reciprocal cross-reactivity, suggesting that A. hebraeum is more immunogenic than A. cajennense. |
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Cross-reactivity between antigens from Amblyomma cajennense and A. hebraeum (Acari: Ixodidae)Amblyomma cajennenseAmblyomma hebraeumAntigensCross-reactivityLaboratory animals exposed to feeding ticks develop resistance which is reflected by a decline in tick engorgement weight, egg-laying by adults and reduced egg viability. Serum antibodies from these hosts and their reaction with tick antigens have been detected by different methods, including precipitation techniques, immunofluorescent techniques, ELISA and Western blots. However, little is known about the effects of antibodies on ticks that engorge on resistant hosts, or which tissues of the tick body are possibly immunogenic. Some researchers, using immunohistochemistry, have detected host antibodies in the gut, salivary glands and haemolymph of ticks engorged on resistant animals. The same technique has helped considerably in determining antigenic sites or antibody target in other arthropods. Consequently, immunohistochemistry techniques were used in this study to detect cross-reactivity between sera raised against Amblyomma cajennense (Fabricius, 1787) with Amblyomma hebraeum (Koch, 1844), and vice versa. The results show the existence of shared antigens between the 2 tick species. In general, our results point more to a 1-way cross-reactivity of A. hebraeum with A. cajennense than a reciprocal cross-reactivity, suggesting that A. hebraeum is more immunogenic than A. cajennense.Parasitology Research Programme Department of Zoology/Entomology University of the Free State, Private Bag X13, Phuthaditjhaba 9866Department of Animal Pathology The State University of Sao Paulo, 14.870-000 Jaboticabal-SPUniv Free State, Parasitol Res Programme, Dept Zool & Entomol, ZA-9866 Phuthaditjhaba, South AfricaState Univ São Paulo, Dept Anim Pathol, BR-14870000 Jaboticabal, SP, BrazilState Univ São Paulo, Dept Anim Pathol, BR-14870000 Jaboticabal, SP, BrazilSouth African Vet AssnUniversity of the Free StateUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Hlatshwayo, M.Szaboó, M. J.P.Bechara, G. H.Mbati, P. A.2022-04-29T08:43:43Z2014-05-20T15:27:38Z2022-04-29T08:43:43Z2014-05-20T15:27:38Z2004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article40-42application/pdfhttp://dx.doi.org/10.4102/jsava.v75i1.447http://jsava.co.za/index.php/jsava/article/view/447Journal of the South African Veterinary Association, v. 75, n. 1, p. 40-42, 2004.Journal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniging. Monument Park: South African Vet Assn, v. 75, n. 1, p. 40-42, 2004.2224-94351019-91280038-2809http://hdl.handle.net/11449/24362410.4102/jsava.v75i1.447WOS:0002221453000092-s2.0-4644242667WOS000222145300009.pdf2382374201685423ScopusWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the South African Veterinary AssociationJournal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniginginfo:eu-repo/semantics/openAccess2024-06-07T13:02:05Zoai:repositorio.unesp.br:11449/243624Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:09:00.278974Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cross-reactivity between antigens from Amblyomma cajennense and A. hebraeum (Acari: Ixodidae) |
title |
Cross-reactivity between antigens from Amblyomma cajennense and A. hebraeum (Acari: Ixodidae) |
spellingShingle |
Cross-reactivity between antigens from Amblyomma cajennense and A. hebraeum (Acari: Ixodidae) Hlatshwayo, M. Amblyomma cajennense Amblyomma hebraeum Antigens Cross-reactivity |
title_short |
Cross-reactivity between antigens from Amblyomma cajennense and A. hebraeum (Acari: Ixodidae) |
title_full |
Cross-reactivity between antigens from Amblyomma cajennense and A. hebraeum (Acari: Ixodidae) |
title_fullStr |
Cross-reactivity between antigens from Amblyomma cajennense and A. hebraeum (Acari: Ixodidae) |
title_full_unstemmed |
Cross-reactivity between antigens from Amblyomma cajennense and A. hebraeum (Acari: Ixodidae) |
title_sort |
Cross-reactivity between antigens from Amblyomma cajennense and A. hebraeum (Acari: Ixodidae) |
author |
Hlatshwayo, M. |
author_facet |
Hlatshwayo, M. Szaboó, M. J.P. Bechara, G. H. Mbati, P. A. |
author_role |
author |
author2 |
Szaboó, M. J.P. Bechara, G. H. Mbati, P. A. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
University of the Free State Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Hlatshwayo, M. Szaboó, M. J.P. Bechara, G. H. Mbati, P. A. |
dc.subject.por.fl_str_mv |
Amblyomma cajennense Amblyomma hebraeum Antigens Cross-reactivity |
topic |
Amblyomma cajennense Amblyomma hebraeum Antigens Cross-reactivity |
description |
Laboratory animals exposed to feeding ticks develop resistance which is reflected by a decline in tick engorgement weight, egg-laying by adults and reduced egg viability. Serum antibodies from these hosts and their reaction with tick antigens have been detected by different methods, including precipitation techniques, immunofluorescent techniques, ELISA and Western blots. However, little is known about the effects of antibodies on ticks that engorge on resistant hosts, or which tissues of the tick body are possibly immunogenic. Some researchers, using immunohistochemistry, have detected host antibodies in the gut, salivary glands and haemolymph of ticks engorged on resistant animals. The same technique has helped considerably in determining antigenic sites or antibody target in other arthropods. Consequently, immunohistochemistry techniques were used in this study to detect cross-reactivity between sera raised against Amblyomma cajennense (Fabricius, 1787) with Amblyomma hebraeum (Koch, 1844), and vice versa. The results show the existence of shared antigens between the 2 tick species. In general, our results point more to a 1-way cross-reactivity of A. hebraeum with A. cajennense than a reciprocal cross-reactivity, suggesting that A. hebraeum is more immunogenic than A. cajennense. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 2014-05-20T15:27:38Z 2014-05-20T15:27:38Z 2022-04-29T08:43:43Z 2022-04-29T08:43:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4102/jsava.v75i1.447 http://jsava.co.za/index.php/jsava/article/view/447 Journal of the South African Veterinary Association, v. 75, n. 1, p. 40-42, 2004. Journal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniging. Monument Park: South African Vet Assn, v. 75, n. 1, p. 40-42, 2004. 2224-9435 1019-9128 0038-2809 http://hdl.handle.net/11449/243624 10.4102/jsava.v75i1.447 WOS:000222145300009 2-s2.0-4644242667 WOS000222145300009.pdf 2382374201685423 |
url |
http://dx.doi.org/10.4102/jsava.v75i1.447 http://jsava.co.za/index.php/jsava/article/view/447 http://hdl.handle.net/11449/243624 |
identifier_str_mv |
Journal of the South African Veterinary Association, v. 75, n. 1, p. 40-42, 2004. Journal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniging. Monument Park: South African Vet Assn, v. 75, n. 1, p. 40-42, 2004. 2224-9435 1019-9128 0038-2809 10.4102/jsava.v75i1.447 WOS:000222145300009 2-s2.0-4644242667 WOS000222145300009.pdf 2382374201685423 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of the South African Veterinary Association Journal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniging |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
40-42 application/pdf |
dc.publisher.none.fl_str_mv |
South African Vet Assn |
publisher.none.fl_str_mv |
South African Vet Assn |
dc.source.none.fl_str_mv |
Scopus Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128762913488896 |