Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.chemosphere.2020.129014 http://hdl.handle.net/11449/207085 |
Resumo: | The liver of anurans play an important role in metabolism, including detoxification, the biotransformation of molecules, and the storage of metabolites. Surfactants are part of domestic and industrial effluents. The effects of linear alkylbenzene sulfonate (LAS) on anuran liver remain unknown, however, some studies have evaluated the effects of LAS on the skin, gills, heart, testes, and liver of fishes. Here, we tested the hypothesis that LAS is hepatotoxic, promoting morphometric alterations in hepatocytes along with inflammation in the tissue, altering hepatic catabolism. We evaluated the effects of a LAS concentration that is considered environmentally safe in Brazilian inland waters on the liver of Lithobates catesbeianus tadpoles, including studies on morphology, morphometry, immunology, and metabolism. LAS exposure promoted enlargement of liver sinusoids and vacuolization of hepatocytes. Exposure to LAS also increased the area of mast cells and melanomacrophages (MMs). Additionally, LAS exposure increased hemosiderin inside MMs, suggesting alterations in the catabolism and storage of iron. Hepatocyte size increased after exposure to LAS, suggesting cytotoxic effects. Integrative analyses (i.e., morphometric, metabolic, and immunological) demonstrated hepatotoxic effects of LAS. These types of studies are key to understanding the negative effects of these substances on tadpole health, as these liver alterations impair anuran homeostasis. |
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Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpolesHemosiderinHepatic melanomacrophagesHepatocytesImmune cellsMorphometrySodium dodecylbenzene sulfonateSurfactantsThe liver of anurans play an important role in metabolism, including detoxification, the biotransformation of molecules, and the storage of metabolites. Surfactants are part of domestic and industrial effluents. The effects of linear alkylbenzene sulfonate (LAS) on anuran liver remain unknown, however, some studies have evaluated the effects of LAS on the skin, gills, heart, testes, and liver of fishes. Here, we tested the hypothesis that LAS is hepatotoxic, promoting morphometric alterations in hepatocytes along with inflammation in the tissue, altering hepatic catabolism. We evaluated the effects of a LAS concentration that is considered environmentally safe in Brazilian inland waters on the liver of Lithobates catesbeianus tadpoles, including studies on morphology, morphometry, immunology, and metabolism. LAS exposure promoted enlargement of liver sinusoids and vacuolization of hepatocytes. Exposure to LAS also increased the area of mast cells and melanomacrophages (MMs). Additionally, LAS exposure increased hemosiderin inside MMs, suggesting alterations in the catabolism and storage of iron. Hepatocyte size increased after exposure to LAS, suggesting cytotoxic effects. Integrative analyses (i.e., morphometric, metabolic, and immunological) demonstrated hepatotoxic effects of LAS. These types of studies are key to understanding the negative effects of these substances on tadpole health, as these liver alterations impair anuran homeostasis.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)British and Foreign School SocietyLaboratório de Fisiologia da Conservação (LAFISC) Universidade Federal de São Carlos (UFSCar)Departamento de Biologia Universidade Estadual Paulista (UNESP), São José do Rio PretoLaboratório de Patologia Experimental (LAPEx) Instituto de Biociências (INBIO) Universidade Federal de Mato Grosso do Sul UFMSLaboratório de Ecotoxicologia e Biomarcadores Em Animais (LEBA) Universidade Federal de São Carlos, SorocabaLaboratório de Biologia Estrutural e Funcional Universidade Federal de São Carlos, SorocabaLaboratório de Microbiologia Ambiental (LAMA) Universidade Federal de São Carlos, SorocabaRECETOX Faculty of Science Masaryk University, Kamenice 753/5, Pavilion A29Departamento de Biologia Universidade Estadual Paulista (UNESP), São José do Rio PretoCNPq: #573921/2008–3FAPESP: 08/57712–4FAPESP: 2014/00946–4British and Foreign School Society: 304552/2019–4CNPq: 304552/2019–4CNPq: 573921/2008–3Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Masaryk UniversityFranco-Belussi, Lilian [UNESP]Jones-Costa, MonicaSalla, Raquel FernandaSouza, Bruno Fernando SantosPinto-Vidal, Felipe AugustoOliveira, Cristiane RonchiSilva-Zacarin, Elaine Cristina MathiasAbdalla, Fabio CamargoDuarte, Iolanda Cristina SilveiraDe Oliveira, Classius [UNESP]2021-06-25T10:48:40Z2021-06-25T10:48:40Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.chemosphere.2020.129014Chemosphere, v. 266.1879-12980045-6535http://hdl.handle.net/11449/20708510.1016/j.chemosphere.2020.1290142-s2.0-8509892344157318566502178590000-0002-2029-7900Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphereinfo:eu-repo/semantics/openAccess2021-10-23T16:15:51Zoai:repositorio.unesp.br:11449/207085Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T16:15:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles |
title |
Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles |
spellingShingle |
Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles Franco-Belussi, Lilian [UNESP] Hemosiderin Hepatic melanomacrophages Hepatocytes Immune cells Morphometry Sodium dodecylbenzene sulfonate Surfactants |
title_short |
Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles |
title_full |
Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles |
title_fullStr |
Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles |
title_full_unstemmed |
Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles |
title_sort |
Hepatotoxicity of the anionic surfactant linear alkylbenzene sulphonate (LAS) in bullfrog tadpoles |
author |
Franco-Belussi, Lilian [UNESP] |
author_facet |
Franco-Belussi, Lilian [UNESP] Jones-Costa, Monica Salla, Raquel Fernanda Souza, Bruno Fernando Santos Pinto-Vidal, Felipe Augusto Oliveira, Cristiane Ronchi Silva-Zacarin, Elaine Cristina Mathias Abdalla, Fabio Camargo Duarte, Iolanda Cristina Silveira De Oliveira, Classius [UNESP] |
author_role |
author |
author2 |
Jones-Costa, Monica Salla, Raquel Fernanda Souza, Bruno Fernando Santos Pinto-Vidal, Felipe Augusto Oliveira, Cristiane Ronchi Silva-Zacarin, Elaine Cristina Mathias Abdalla, Fabio Camargo Duarte, Iolanda Cristina Silveira De Oliveira, Classius [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso do Sul (UFMS) Masaryk University |
dc.contributor.author.fl_str_mv |
Franco-Belussi, Lilian [UNESP] Jones-Costa, Monica Salla, Raquel Fernanda Souza, Bruno Fernando Santos Pinto-Vidal, Felipe Augusto Oliveira, Cristiane Ronchi Silva-Zacarin, Elaine Cristina Mathias Abdalla, Fabio Camargo Duarte, Iolanda Cristina Silveira De Oliveira, Classius [UNESP] |
dc.subject.por.fl_str_mv |
Hemosiderin Hepatic melanomacrophages Hepatocytes Immune cells Morphometry Sodium dodecylbenzene sulfonate Surfactants |
topic |
Hemosiderin Hepatic melanomacrophages Hepatocytes Immune cells Morphometry Sodium dodecylbenzene sulfonate Surfactants |
description |
The liver of anurans play an important role in metabolism, including detoxification, the biotransformation of molecules, and the storage of metabolites. Surfactants are part of domestic and industrial effluents. The effects of linear alkylbenzene sulfonate (LAS) on anuran liver remain unknown, however, some studies have evaluated the effects of LAS on the skin, gills, heart, testes, and liver of fishes. Here, we tested the hypothesis that LAS is hepatotoxic, promoting morphometric alterations in hepatocytes along with inflammation in the tissue, altering hepatic catabolism. We evaluated the effects of a LAS concentration that is considered environmentally safe in Brazilian inland waters on the liver of Lithobates catesbeianus tadpoles, including studies on morphology, morphometry, immunology, and metabolism. LAS exposure promoted enlargement of liver sinusoids and vacuolization of hepatocytes. Exposure to LAS also increased the area of mast cells and melanomacrophages (MMs). Additionally, LAS exposure increased hemosiderin inside MMs, suggesting alterations in the catabolism and storage of iron. Hepatocyte size increased after exposure to LAS, suggesting cytotoxic effects. Integrative analyses (i.e., morphometric, metabolic, and immunological) demonstrated hepatotoxic effects of LAS. These types of studies are key to understanding the negative effects of these substances on tadpole health, as these liver alterations impair anuran homeostasis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:48:40Z 2021-06-25T10:48:40Z 2021-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.chemosphere.2020.129014 Chemosphere, v. 266. 1879-1298 0045-6535 http://hdl.handle.net/11449/207085 10.1016/j.chemosphere.2020.129014 2-s2.0-85098923441 5731856650217859 0000-0002-2029-7900 |
url |
http://dx.doi.org/10.1016/j.chemosphere.2020.129014 http://hdl.handle.net/11449/207085 |
identifier_str_mv |
Chemosphere, v. 266. 1879-1298 0045-6535 10.1016/j.chemosphere.2020.129014 2-s2.0-85098923441 5731856650217859 0000-0002-2029-7900 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemosphere |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803046356610187264 |