Prevalence and etiology of buffalo mastitis and milk somatic cell countin dry and rainy seasons in a buffalo herd from Analândia, São Paulo State,Brazil

• Autor(a) principal: Pizauro, L. J. I. [UNESP]
• Data de Publicação: 2014
• Outros Autores: Silva, D. G. [UNESP], Santana, A. M. [UNESP], Clemente, V., Lara, G. H. B. [UNESP], Listoni, F. J. P., Vaz, A. C. N., Vidal-martins, A. M. C., Ribeiro, M. G., Fagliari, J. J.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000601703&lng=en&nrm=iso&tlng=en; http://hdl.handle.net/11449/127413
• topic: Antibiogram; Bubalus bubalis; SCC; Mammary gland; Microorganisms; Antibiograma; Bubalus bubalis; CCS; Glândula mamária; Micro-organismos

The aim of this study was to evaluate somatic cell count (SCC), prevalence andetiology of mastitis in a dairy buffalo herd from Analândia, São Paulo State, Brazil,in the dry and rainy seasons. Additionally, antimicrobial susceptibility profile ofmicroorganisms isolated from milk samples was also evaluated. 1,042 milk samples fromfemale Murrah buffaloes in a dairy farm located in Analândia, São Paulo State,Brazil, collected between May 2011 and November 2012 were analyzed. After the mammarygland physical examination, strip cup test and California Mastitis Test (CMT) wereperformed. Afterwards, 50mL of milk samples from each mammary quarter were collectedaseptically for SCC in automatic equipment and microbiological examination. Theantimicrobial sensitivity profile to ampicillin, cefoperazone, ceftiofur,enrofloxacin, gentamicin, neomycin, oxacillin, penicillin, andsulfamethoxazole/trimethoprim was evaluated by disk diffusion method. The monthlyaverage temperature and pluviometric index were obtained from Centro Integrado deInformações Agrometeorológicas (CIIAGRO) of Instituto Agronômico de Campina(IAC). Milk samples with positive results in the microbiological test showed averageSCC of 137,720 cells/mL in the dry period and 190,309 cells/mL in the rainy period.Although a higher number of isolated microorganisms was observed in buffalo milksamples during the rainy period (69/600) compared to the dry period (50/442), theseason had no significant effect on the frequency of isolation of microorganisms. Themain genera of microorganisms isolated were coagulase-negative Staphylococcus (38.4%), Streptococcus agalactiae(28.8%), and Bacillus spp. (7.56%) during the dry season and Corynebacterium sp. (23.5%), Streptococcus spp.(32.3%), and Streptococcus agalactiae (9.24%) during the rainyperiod. Multidrug resistance was observed in 30.1% of the isolatedmicroorganisms.