Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000518697 http://hdl.handle.net/11449/229755 |
Resumo: | Background: Chagasic megaesophagus (CM) as well as the presence of human papillomavirus (HPV) has been reported as etiological factors for esophageal squamous cell carcinoma (ESCC). Objective: We assessed the prevalence of HPV DNA in a series of ESCCs associated or not with CM. Data obtained were further correlated to the pathological and clinical data of affected individuals. Methods: A retrospective study was performed on 92 formalin-fixed and paraffin-embedded tissues collected from patients referred to 3 different hospitals in São Paulo, Brazil: Barretos Cancer Hospital, Barretos, São Paulo; Federal University of Triângulo Mineiro, Uberaba, Minas Gerais; and São Paulo State University, Botucatu, São Paulo. Cases were divided into 3 groups: (i) 24 patients with CM associated with ESCC (CM/ESCC); (ii) 37 patients with ESCC without CM (ESCC); and (iii) 31 patients with CM without ESCC (CM). Detection of HPV DNA was assessed in all samples by a genotyping assay combining multiplex polymerase chain reaction and bead-based Luminex technology. Results: We identified a high prevalence of high-risk HPV in patients in the CM group (12/31, 38.8%) and CM/ESCC (8/24, 33.3%), compared to individuals in the ESCC group (6/37, 16.3%). The individuals in the groups with cancer (ESCC and CM/ESCC) had a higher frequency of HPV-16 (4/9, 44.5% and 2/8, 25.0%). The other types of high-risk HPVs detected were HPV-31, 45, 51, 53, 56, 66, and 73. We also observed in some samples HPV coinfection by more than one viral type. Despite the high incidence of HPV, it did not show any association with the patient's clinical-pathological and molecular (TP53 mutation status) characteristics. Conclusion: This is the first report of the presence of HPV DNA in CM associated with ESCC. HPV infection was more presence in megaesophagus lesions. Further studies are needed to confirm and better understand the role of persistent HPV infection in patients with CM. |
id |
UNSP_861bb1c588896b8e59a6507a3a01b631 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/229755 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell CarcinomaBrazilChagas diseaseChagasic megaesophagusEsophageal cancerEsophageal squamous cell carcinomaHuman papillomavirusBackground: Chagasic megaesophagus (CM) as well as the presence of human papillomavirus (HPV) has been reported as etiological factors for esophageal squamous cell carcinoma (ESCC). Objective: We assessed the prevalence of HPV DNA in a series of ESCCs associated or not with CM. Data obtained were further correlated to the pathological and clinical data of affected individuals. Methods: A retrospective study was performed on 92 formalin-fixed and paraffin-embedded tissues collected from patients referred to 3 different hospitals in São Paulo, Brazil: Barretos Cancer Hospital, Barretos, São Paulo; Federal University of Triângulo Mineiro, Uberaba, Minas Gerais; and São Paulo State University, Botucatu, São Paulo. Cases were divided into 3 groups: (i) 24 patients with CM associated with ESCC (CM/ESCC); (ii) 37 patients with ESCC without CM (ESCC); and (iii) 31 patients with CM without ESCC (CM). Detection of HPV DNA was assessed in all samples by a genotyping assay combining multiplex polymerase chain reaction and bead-based Luminex technology. Results: We identified a high prevalence of high-risk HPV in patients in the CM group (12/31, 38.8%) and CM/ESCC (8/24, 33.3%), compared to individuals in the ESCC group (6/37, 16.3%). The individuals in the groups with cancer (ESCC and CM/ESCC) had a higher frequency of HPV-16 (4/9, 44.5% and 2/8, 25.0%). The other types of high-risk HPVs detected were HPV-31, 45, 51, 53, 56, 66, and 73. We also observed in some samples HPV coinfection by more than one viral type. Despite the high incidence of HPV, it did not show any association with the patient's clinical-pathological and molecular (TP53 mutation status) characteristics. Conclusion: This is the first report of the presence of HPV DNA in CM associated with ESCC. HPV infection was more presence in megaesophagus lesions. Further studies are needed to confirm and better understand the role of persistent HPV infection in patients with CM.Molecular Oncology Research Center Barretos Cancer HospitalCenter for Translational Research in Oncology Instituto Do Cancer Do Estado de Sao Paulo-ICESP, Cerqueira CésarDepartment of Digestive Surgery Barretos Cancer HospitalDepartment of Pathology Diagnosis of Biopsies and Surgical Specimens Barretos Cancer HospitalDepartment of Digestive Surgery and Pathology Medical School UFTM Federal University of Triangulo MineiroDepartament of Gastroenterology Surgery and Pathology Medical School UNESP São Paulo State UniversityDepartment of Endoscopy Barretos Cancer Hospital BarretosLife and Health Sciences Research Institute (ICVS) School of Medicine University of MinhoICVS/3B's-PT Government Associate Laboratory, GuimarãesDepartment of Radiology and Oncology Faculdade de Medicina Universidade de Sao PauloDepartment of Radiology and Oncology Medical School University of São Paulo, ButantaMedical Laboratory of Medical Investigation (LIM) 14 Department of Pathology Medical School University of São Paulo, ButantaDepartament of Gastroenterology Surgery and Pathology Medical School UNESP São Paulo State UniversityBarretos Cancer HospitalInstituto Do Cancer Do Estado de Sao Paulo-ICESPFederal University of Triangulo MineiroUniversidade Estadual Paulista (UNESP)BarretosUniversity of MinhoICVS/3B's-PT Government Associate LaboratoryUniversidade de São Paulo (USP)Munari, Fernanda FrancoSichero, LauraCarloni, Adriana CruvinelLacerda, Croider FrancoNunes, Emily MontosaDe Oliveira, Antônio Talvane TorresScapulatempo-Neto, CristovamDa Silva, Sandra Regina MoriniCrema, EduardoAdad, Sheila Jorge [UNESP]Rodrigues, Maria Aparecida Marchesan [UNESP]Henry, Maria Aparecida Coelho Arruda [UNESP]Guimarães, Denise PeixotoReis, Rui ManuelVilla, Luisa LinaLongatto-Filho, Adhemar2022-04-29T08:35:38Z2022-04-29T08:35:38Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article29-37http://dx.doi.org/10.1159/000518697Pathobiology, v. 89, n. 1, p. 29-37, 2022.1423-02911015-2008http://hdl.handle.net/11449/22975510.1159/0005186972-s2.0-85117521323Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathobiologyinfo:eu-repo/semantics/openAccess2022-04-29T08:35:38Zoai:repositorio.unesp.br:11449/229755Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:35:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma |
title |
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma |
spellingShingle |
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma Munari, Fernanda Franco Brazil Chagas disease Chagasic megaesophagus Esophageal cancer Esophageal squamous cell carcinoma Human papillomavirus |
title_short |
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma |
title_full |
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma |
title_fullStr |
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma |
title_full_unstemmed |
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma |
title_sort |
Frequency of Human Papillomavirus Detection in Chagasic Megaesophagus Associated or Not with Esophageal Squamous Cell Carcinoma |
author |
Munari, Fernanda Franco |
author_facet |
Munari, Fernanda Franco Sichero, Laura Carloni, Adriana Cruvinel Lacerda, Croider Franco Nunes, Emily Montosa De Oliveira, Antônio Talvane Torres Scapulatempo-Neto, Cristovam Da Silva, Sandra Regina Morini Crema, Eduardo Adad, Sheila Jorge [UNESP] Rodrigues, Maria Aparecida Marchesan [UNESP] Henry, Maria Aparecida Coelho Arruda [UNESP] Guimarães, Denise Peixoto Reis, Rui Manuel Villa, Luisa Lina Longatto-Filho, Adhemar |
author_role |
author |
author2 |
Sichero, Laura Carloni, Adriana Cruvinel Lacerda, Croider Franco Nunes, Emily Montosa De Oliveira, Antônio Talvane Torres Scapulatempo-Neto, Cristovam Da Silva, Sandra Regina Morini Crema, Eduardo Adad, Sheila Jorge [UNESP] Rodrigues, Maria Aparecida Marchesan [UNESP] Henry, Maria Aparecida Coelho Arruda [UNESP] Guimarães, Denise Peixoto Reis, Rui Manuel Villa, Luisa Lina Longatto-Filho, Adhemar |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Barretos Cancer Hospital Instituto Do Cancer Do Estado de Sao Paulo-ICESP Federal University of Triangulo Mineiro Universidade Estadual Paulista (UNESP) Barretos University of Minho ICVS/3B's-PT Government Associate Laboratory Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Munari, Fernanda Franco Sichero, Laura Carloni, Adriana Cruvinel Lacerda, Croider Franco Nunes, Emily Montosa De Oliveira, Antônio Talvane Torres Scapulatempo-Neto, Cristovam Da Silva, Sandra Regina Morini Crema, Eduardo Adad, Sheila Jorge [UNESP] Rodrigues, Maria Aparecida Marchesan [UNESP] Henry, Maria Aparecida Coelho Arruda [UNESP] Guimarães, Denise Peixoto Reis, Rui Manuel Villa, Luisa Lina Longatto-Filho, Adhemar |
dc.subject.por.fl_str_mv |
Brazil Chagas disease Chagasic megaesophagus Esophageal cancer Esophageal squamous cell carcinoma Human papillomavirus |
topic |
Brazil Chagas disease Chagasic megaesophagus Esophageal cancer Esophageal squamous cell carcinoma Human papillomavirus |
description |
Background: Chagasic megaesophagus (CM) as well as the presence of human papillomavirus (HPV) has been reported as etiological factors for esophageal squamous cell carcinoma (ESCC). Objective: We assessed the prevalence of HPV DNA in a series of ESCCs associated or not with CM. Data obtained were further correlated to the pathological and clinical data of affected individuals. Methods: A retrospective study was performed on 92 formalin-fixed and paraffin-embedded tissues collected from patients referred to 3 different hospitals in São Paulo, Brazil: Barretos Cancer Hospital, Barretos, São Paulo; Federal University of Triângulo Mineiro, Uberaba, Minas Gerais; and São Paulo State University, Botucatu, São Paulo. Cases were divided into 3 groups: (i) 24 patients with CM associated with ESCC (CM/ESCC); (ii) 37 patients with ESCC without CM (ESCC); and (iii) 31 patients with CM without ESCC (CM). Detection of HPV DNA was assessed in all samples by a genotyping assay combining multiplex polymerase chain reaction and bead-based Luminex technology. Results: We identified a high prevalence of high-risk HPV in patients in the CM group (12/31, 38.8%) and CM/ESCC (8/24, 33.3%), compared to individuals in the ESCC group (6/37, 16.3%). The individuals in the groups with cancer (ESCC and CM/ESCC) had a higher frequency of HPV-16 (4/9, 44.5% and 2/8, 25.0%). The other types of high-risk HPVs detected were HPV-31, 45, 51, 53, 56, 66, and 73. We also observed in some samples HPV coinfection by more than one viral type. Despite the high incidence of HPV, it did not show any association with the patient's clinical-pathological and molecular (TP53 mutation status) characteristics. Conclusion: This is the first report of the presence of HPV DNA in CM associated with ESCC. HPV infection was more presence in megaesophagus lesions. Further studies are needed to confirm and better understand the role of persistent HPV infection in patients with CM. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:35:38Z 2022-04-29T08:35:38Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000518697 Pathobiology, v. 89, n. 1, p. 29-37, 2022. 1423-0291 1015-2008 http://hdl.handle.net/11449/229755 10.1159/000518697 2-s2.0-85117521323 |
url |
http://dx.doi.org/10.1159/000518697 http://hdl.handle.net/11449/229755 |
identifier_str_mv |
Pathobiology, v. 89, n. 1, p. 29-37, 2022. 1423-0291 1015-2008 10.1159/000518697 2-s2.0-85117521323 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathobiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
29-37 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965383552139264 |