Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocomposites

Detalhes bibliográficos
Autor(a) principal: Domeneguetti, Rafael R. [UNESP]
Data de Publicação: 2023
Outros Autores: Sakai, Vanessa Y., Perotti, Gustavo F., Silva, Isabel C. [UNESP], Tercjak, Agnieszka, Barud, Hernane S., Pavan, Fernando [UNESP], Constantino, Vera R.L., Ribeiro, Sidney J. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.clay.2023.106851
http://hdl.handle.net/11449/248398
Resumo: Bacterial cellulose (BC) membranes were produced in culture media containing Komagataeibacter xylinus microorganism and four different concentrations of Laponite (Lap) XLG up to 1.0% (m/v) to evaluate the availability of in-situ method for production of BC/Lap nanocomposites. The effect of Lap on the structure and some properties of the biosynthesised polymer was evaluated by thermogravimetric analysis coupled to mass spectrometry (TGA-MS), Fourier Transform infrared (FT-IR) and Raman (FT-Raman) vibrational spectroscopies, X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy dispersive X-ray spectroscopy (EDS) and atomic force microscopy (AFM). TGA-MS and vibrational spectroscopy indicated that Lap particles were not incorporated into the BC membranes using 0.10 or 0.25% of the inorganic phase whereas all obtained results showed that its presence had a strong influence on the BC properties. This result can be an effect of the interaction of hydrophilic Lap particles with elementary fibrils of cellulose, expelled from the bacteria, fostering the disruption of chains organization. However, the 3D hierarchical organization of BC at the microscale was not collapsed according to SEM and AFM images. As the amount of Lap increased in the culture medium and in the produced membrane, the ribbons became less defined, suggesting the predominance of thinner ribbons. The transparency of the membranes was enhanced when the nanoclay loading was raised. The presence of high clay loadings in the BC/Lap nanocomposite did not negatively impact the cell viability against keratinocytes in comparison to pristine BC membranes, opening new opportunities to explore such nanocomposites for biomedical, pharmaceutical and cosmetics uses.
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spelling Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocompositesBiopolymersBiosynthesised nanocompositeCellulose-synthesizing bacteriaHectoriteLayered magnesium silicatePolymer-clay nanocompositesBacterial cellulose (BC) membranes were produced in culture media containing Komagataeibacter xylinus microorganism and four different concentrations of Laponite (Lap) XLG up to 1.0% (m/v) to evaluate the availability of in-situ method for production of BC/Lap nanocomposites. The effect of Lap on the structure and some properties of the biosynthesised polymer was evaluated by thermogravimetric analysis coupled to mass spectrometry (TGA-MS), Fourier Transform infrared (FT-IR) and Raman (FT-Raman) vibrational spectroscopies, X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy dispersive X-ray spectroscopy (EDS) and atomic force microscopy (AFM). TGA-MS and vibrational spectroscopy indicated that Lap particles were not incorporated into the BC membranes using 0.10 or 0.25% of the inorganic phase whereas all obtained results showed that its presence had a strong influence on the BC properties. This result can be an effect of the interaction of hydrophilic Lap particles with elementary fibrils of cellulose, expelled from the bacteria, fostering the disruption of chains organization. However, the 3D hierarchical organization of BC at the microscale was not collapsed according to SEM and AFM images. As the amount of Lap increased in the culture medium and in the produced membrane, the ribbons became less defined, suggesting the predominance of thinner ribbons. The transparency of the membranes was enhanced when the nanoclay loading was raised. The presence of high clay loadings in the BC/Lap nanocomposite did not negatively impact the cell viability against keratinocytes in comparison to pristine BC membranes, opening new opportunities to explore such nanocomposites for biomedical, pharmaceutical and cosmetics uses.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Química Inorgânica Instituto de Química Universidade Estadual Paulista (UNESP), Rua Prof. Francisco Degni, 55 Bairro Quitandinha, SPDepartamento de Química Fundamental Instituto de Química Universidade de São Paulo (USP), Av. Prof. Lineu Prestes, 748, Cidade Universitária, SPInstituto de Ciências Exatas e Tecnologia Universidade Federal do Amazonas (UFAM), Rua Nossa Senhora do Rosário, 3863, Bairro Tiradentes, AMDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (UNESP), Rodovia Araraquara Jaú, Km 01 - s/n, Bairro Campos Ville, SPGroup ‘Materials + Technologies´ (GMT) Department of Chemical and Environmental Engineering Faculty of Engineering Gipuzkoa University of the Basque Country (UPV/EHU), Plaza Europa 1Laboratório de BioPolímeros e Biomateriais (BioPolMat/Uniara), Rua Carlos Gomes, 1338, Bairro Centro, SPDepartamento de Química Inorgânica Instituto de Química Universidade Estadual Paulista (UNESP), Rua Prof. Francisco Degni, 55 Bairro Quitandinha, SPDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (UNESP), Rodovia Araraquara Jaú, Km 01 - s/n, Bairro Campos Ville, SPFAPESP: 2013/07793-6FAPESP: 2014/50869-6FAPESP: 2018/25512-8FAPESP: 314034/2021-8CNPq: 407822/2018-6CNPq: INCT-INFOUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Universidade Federal do Amazonas (UFAM)University of the Basque Country (UPV/EHU)Laboratório de BioPolímeros e Biomateriais (BioPolMat/Uniara)Domeneguetti, Rafael R. [UNESP]Sakai, Vanessa Y.Perotti, Gustavo F.Silva, Isabel C. [UNESP]Tercjak, AgnieszkaBarud, Hernane S.Pavan, Fernando [UNESP]Constantino, Vera R.L.Ribeiro, Sidney J. [UNESP]2023-07-29T13:42:56Z2023-07-29T13:42:56Z2023-03-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.clay.2023.106851Applied Clay Science, v. 234.0169-1317http://hdl.handle.net/11449/24839810.1016/j.clay.2023.1068512-s2.0-85148541547Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Clay Scienceinfo:eu-repo/semantics/openAccess2023-07-29T13:42:56Zoai:repositorio.unesp.br:11449/248398Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:42:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocomposites
title Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocomposites
spellingShingle Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocomposites
Domeneguetti, Rafael R. [UNESP]
Biopolymers
Biosynthesised nanocomposite
Cellulose-synthesizing bacteria
Hectorite
Layered magnesium silicate
Polymer-clay nanocomposites
title_short Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocomposites
title_full Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocomposites
title_fullStr Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocomposites
title_full_unstemmed Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocomposites
title_sort Structural and morphological properties of in-situ biosynthesis of biocompatible bacterial cellulose/Laponite nanocomposites
author Domeneguetti, Rafael R. [UNESP]
author_facet Domeneguetti, Rafael R. [UNESP]
Sakai, Vanessa Y.
Perotti, Gustavo F.
Silva, Isabel C. [UNESP]
Tercjak, Agnieszka
Barud, Hernane S.
Pavan, Fernando [UNESP]
Constantino, Vera R.L.
Ribeiro, Sidney J. [UNESP]
author_role author
author2 Sakai, Vanessa Y.
Perotti, Gustavo F.
Silva, Isabel C. [UNESP]
Tercjak, Agnieszka
Barud, Hernane S.
Pavan, Fernando [UNESP]
Constantino, Vera R.L.
Ribeiro, Sidney J. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
Universidade Federal do Amazonas (UFAM)
University of the Basque Country (UPV/EHU)
Laboratório de BioPolímeros e Biomateriais (BioPolMat/Uniara)
dc.contributor.author.fl_str_mv Domeneguetti, Rafael R. [UNESP]
Sakai, Vanessa Y.
Perotti, Gustavo F.
Silva, Isabel C. [UNESP]
Tercjak, Agnieszka
Barud, Hernane S.
Pavan, Fernando [UNESP]
Constantino, Vera R.L.
Ribeiro, Sidney J. [UNESP]
dc.subject.por.fl_str_mv Biopolymers
Biosynthesised nanocomposite
Cellulose-synthesizing bacteria
Hectorite
Layered magnesium silicate
Polymer-clay nanocomposites
topic Biopolymers
Biosynthesised nanocomposite
Cellulose-synthesizing bacteria
Hectorite
Layered magnesium silicate
Polymer-clay nanocomposites
description Bacterial cellulose (BC) membranes were produced in culture media containing Komagataeibacter xylinus microorganism and four different concentrations of Laponite (Lap) XLG up to 1.0% (m/v) to evaluate the availability of in-situ method for production of BC/Lap nanocomposites. The effect of Lap on the structure and some properties of the biosynthesised polymer was evaluated by thermogravimetric analysis coupled to mass spectrometry (TGA-MS), Fourier Transform infrared (FT-IR) and Raman (FT-Raman) vibrational spectroscopies, X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy dispersive X-ray spectroscopy (EDS) and atomic force microscopy (AFM). TGA-MS and vibrational spectroscopy indicated that Lap particles were not incorporated into the BC membranes using 0.10 or 0.25% of the inorganic phase whereas all obtained results showed that its presence had a strong influence on the BC properties. This result can be an effect of the interaction of hydrophilic Lap particles with elementary fibrils of cellulose, expelled from the bacteria, fostering the disruption of chains organization. However, the 3D hierarchical organization of BC at the microscale was not collapsed according to SEM and AFM images. As the amount of Lap increased in the culture medium and in the produced membrane, the ribbons became less defined, suggesting the predominance of thinner ribbons. The transparency of the membranes was enhanced when the nanoclay loading was raised. The presence of high clay loadings in the BC/Lap nanocomposite did not negatively impact the cell viability against keratinocytes in comparison to pristine BC membranes, opening new opportunities to explore such nanocomposites for biomedical, pharmaceutical and cosmetics uses.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:42:56Z
2023-07-29T13:42:56Z
2023-03-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.clay.2023.106851
Applied Clay Science, v. 234.
0169-1317
http://hdl.handle.net/11449/248398
10.1016/j.clay.2023.106851
2-s2.0-85148541547
url http://dx.doi.org/10.1016/j.clay.2023.106851
http://hdl.handle.net/11449/248398
identifier_str_mv Applied Clay Science, v. 234.
0169-1317
10.1016/j.clay.2023.106851
2-s2.0-85148541547
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Applied Clay Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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