Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jmv.26985 http://hdl.handle.net/11449/209317 |
Resumo: | One year into the coronavirus disease 2019 (COVID-19) pandemic, diagnostic strategies, although central for contact tracing and other preventive measures, are still limited. To meet the global demand, lower cost and faster antigen tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection are a convenient alternative to the gold standard reverse transcription-polymerase chain reaction (RT-PCR) assay. We tested laboratory-based RT-PCR RNA detection and two rapid antigen detection (RAD) tests, based on the immunochromatography test for nucleocapsid protein of SARS-CoV-2 (COVID-19 Ag ECO Test, ECO Diagnostica, and Panbio COVID-19 Ag Rapid Test Abbott). Paired collection and testing were done in a small prospective open study in three clinical services in Sao Paulo, constituted of mostly symptomatic volunteers at collection (97%, 109/112) for a median of 4 days (interquartile range: 3-6), ranging from 1 to 30. Among the 108 paired RT-PCR/RAD tests, results were concordant in 96.4% (101/108). The test's performance was comparable, with an overall sensitivity of 87% and a specificity of 96%. These observations add to other data that suggest that antigen tests may provide reasonable sensitivity and specificity and deserve a role to improve testing strategies, especially in resource-limited settings. |
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Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low costCOVID󈙓diagnosticgargle lavagenasopharyngeal swabrapid antigen detectionSARS‐CoV𔂢One year into the coronavirus disease 2019 (COVID-19) pandemic, diagnostic strategies, although central for contact tracing and other preventive measures, are still limited. To meet the global demand, lower cost and faster antigen tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection are a convenient alternative to the gold standard reverse transcription-polymerase chain reaction (RT-PCR) assay. We tested laboratory-based RT-PCR RNA detection and two rapid antigen detection (RAD) tests, based on the immunochromatography test for nucleocapsid protein of SARS-CoV-2 (COVID-19 Ag ECO Test, ECO Diagnostica, and Panbio COVID-19 Ag Rapid Test Abbott). Paired collection and testing were done in a small prospective open study in three clinical services in Sao Paulo, constituted of mostly symptomatic volunteers at collection (97%, 109/112) for a median of 4 days (interquartile range: 3-6), ranging from 1 to 30. Among the 108 paired RT-PCR/RAD tests, results were concordant in 96.4% (101/108). The test's performance was comparable, with an overall sensitivity of 87% and a specificity of 96%. These observations add to other data that suggest that antigen tests may provide reasonable sensitivity and specificity and deserve a role to improve testing strategies, especially in resource-limited settings.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Santo Andre Hlth Secretary, Infect Dis Outpatient Clin, Santo Andre, SP, BrazilAdolfo Lutz Inst, Santo Andre Reg Ctr, Santo Andre, SP, BrazilSao Paulo State Univ, Bioproc & Biotecnol Engn, Botucatu, SP, BrazilAdolfo Lutz Inst, Virol Ctr, Ave Dr Arnaldo 355, BR-01246000 Sao Paulo, SP, BrazilSao Paulo State Univ, Bioproc & Biotecnol Engn, Botucatu, SP, BrazilCNPq: 442776/2019-5FAPESP: 2018/14384-9Wiley-BlackwellSanto Andre Hlth SecretaryAdolfo Lutz InstUniversidade Estadual Paulista (Unesp)Matsuda, Elaine MonteiroCampos, Ivana Barros deOliveira, Isabela Penteriche de [UNESP]Colpas, Daniela RodriguesCarmo, Andreia Moreira dos SantosBrigido, Luis Fernando de Macedo2021-06-25T11:56:07Z2021-06-25T11:56:07Z2021-04-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6http://dx.doi.org/10.1002/jmv.26985Journal Of Medical Virology. Hoboken: Wiley, 6 p., 2021.0146-6615http://hdl.handle.net/11449/20931710.1002/jmv.26985WOS:000637681200001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Medical Virologyinfo:eu-repo/semantics/openAccess2021-10-23T19:28:03Zoai:repositorio.unesp.br:11449/209317Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:28:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost |
title |
Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost |
spellingShingle |
Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost Matsuda, Elaine Monteiro COVID‐ 19 diagnostic gargle lavage nasopharyngeal swab rapid antigen detection SARS‐ CoV‐ 2 |
title_short |
Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost |
title_full |
Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost |
title_fullStr |
Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost |
title_full_unstemmed |
Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost |
title_sort |
Field evaluation of COVID-19 antigen tests versus RNA based detection: Potential lower sensitivity compensated by immediate results, technical simplicity, and low cost |
author |
Matsuda, Elaine Monteiro |
author_facet |
Matsuda, Elaine Monteiro Campos, Ivana Barros de Oliveira, Isabela Penteriche de [UNESP] Colpas, Daniela Rodrigues Carmo, Andreia Moreira dos Santos Brigido, Luis Fernando de Macedo |
author_role |
author |
author2 |
Campos, Ivana Barros de Oliveira, Isabela Penteriche de [UNESP] Colpas, Daniela Rodrigues Carmo, Andreia Moreira dos Santos Brigido, Luis Fernando de Macedo |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Santo Andre Hlth Secretary Adolfo Lutz Inst Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Matsuda, Elaine Monteiro Campos, Ivana Barros de Oliveira, Isabela Penteriche de [UNESP] Colpas, Daniela Rodrigues Carmo, Andreia Moreira dos Santos Brigido, Luis Fernando de Macedo |
dc.subject.por.fl_str_mv |
COVID‐ 19 diagnostic gargle lavage nasopharyngeal swab rapid antigen detection SARS‐ CoV‐ 2 |
topic |
COVID‐ 19 diagnostic gargle lavage nasopharyngeal swab rapid antigen detection SARS‐ CoV‐ 2 |
description |
One year into the coronavirus disease 2019 (COVID-19) pandemic, diagnostic strategies, although central for contact tracing and other preventive measures, are still limited. To meet the global demand, lower cost and faster antigen tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection are a convenient alternative to the gold standard reverse transcription-polymerase chain reaction (RT-PCR) assay. We tested laboratory-based RT-PCR RNA detection and two rapid antigen detection (RAD) tests, based on the immunochromatography test for nucleocapsid protein of SARS-CoV-2 (COVID-19 Ag ECO Test, ECO Diagnostica, and Panbio COVID-19 Ag Rapid Test Abbott). Paired collection and testing were done in a small prospective open study in three clinical services in Sao Paulo, constituted of mostly symptomatic volunteers at collection (97%, 109/112) for a median of 4 days (interquartile range: 3-6), ranging from 1 to 30. Among the 108 paired RT-PCR/RAD tests, results were concordant in 96.4% (101/108). The test's performance was comparable, with an overall sensitivity of 87% and a specificity of 96%. These observations add to other data that suggest that antigen tests may provide reasonable sensitivity and specificity and deserve a role to improve testing strategies, especially in resource-limited settings. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:56:07Z 2021-06-25T11:56:07Z 2021-04-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jmv.26985 Journal Of Medical Virology. Hoboken: Wiley, 6 p., 2021. 0146-6615 http://hdl.handle.net/11449/209317 10.1002/jmv.26985 WOS:000637681200001 |
url |
http://dx.doi.org/10.1002/jmv.26985 http://hdl.handle.net/11449/209317 |
identifier_str_mv |
Journal Of Medical Virology. Hoboken: Wiley, 6 p., 2021. 0146-6615 10.1002/jmv.26985 WOS:000637681200001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Medical Virology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803047376116514816 |