Detecção e caracterização de Escherichia coli potencialmente patogênicas em aves selvagens e pombos-domésticos na cidade de Jaboticabal-SP

• Autor(a) principal: Borges, Clarissa Araújo [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/123679; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-06-2015/000831940.pdf
• topic: Ave; Pombo; Epidemiologia; Saúde pública; Escherichia coli; Zoonoses; Birds

Escherichia coli strains that cause disease outside the intestine are known as extraintestinal pathogenic E. coli (ExPEC) and include uropathogenic E. coli (UPEC), isolated from urinary tract infections, neonatal-meningitis E. coli (NMEC), isolated from human neonates and avian pathogenic E. coli (APEC), responsible for colibacillosis in birds. These pathotypes shows similarities that suggest that the avian strains potentially have zoonotic properties. The aim of this study was to detect and characterize E. coli strains possessing virulence genes related to APEC in wild birds and pigeons, both captivity and free-living, apparently healthy. Of 500 samples collected, there were 49 isolates obtained that were analyzed by PCR for the presence of virulence genes, submitted to pathogenicity test in one day old chicks, to antimicrobial susceptibility test, serotyping, phylogenetic typing and PFGE. Virulence genes, somatic and flagellar antigens found in the isolates were previously associated to high pathogenic APEC and were also detected in human extraintestinal infections strains. Most of the isolates belonged to phylogenetic groups B1 and B2, previously described as groups that harbor pathogenic isolates from animals and the ones that cause extraintestinal infections, respectively. Furthermore, these groups had isolates with the highest number of virulence genes and highly pathogenic, while most strains of group D were no pathogenic. The captivity pigeons showed more multidrug-resistant isolates (100.0%), followed by domestic wild birds (75.0%), free-living wild birds (53.3%) and free-living pigeons (26.3%). The isolates showed high heterogeneity by PFGE, indicating that there were not E. coli prevalent clones among these birds. The results showed that wild birds and pigeons are reservoirs of multidrug-resistant E. coli harboring virulence genes related to APEC, being potentially pathogenic for humans and other animals, being able to ...