Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acsomega.0c01716 http://hdl.handle.net/11449/201900 |
Resumo: | The supply of food derived from aquaculture has increased significantly in recent years. The aim of this industrial sector is to produce sustainable products to meet the needs of consumers, providing food security and nutritional benefits. The development of aquaculture has faced challenges including disease outbreaks that can cause substantial economic losses. These diseases can be controlled using chemicals such as antibiotics. However, the indiscriminate use of these substances can have major negative impacts on human health and the environment with the additional risk of the emergence of resistant organisms. The present manuscript describes the use of phytotherapy in association with nanotechnology in order to obtain a more effective and less harmful system for the control of bacterial diseases in fish. Zein nanoparticles associated with eugenol and garlic essential oil were prepared through antisolvent precipitation and characterized. Zein nanoparticles are promising carrier systems as zein proteins are biodegradable and biocompatible and, in this way, good candidates for encapsulation of active ingredients. The system presented good physicochemical properties with an average particle diameter of approximately 150 nm, a polydispersity index lower than 0.2, and a zeta potential of approximately 30 mV. High encapsulation efficiency was obtained for the active compounds with values higher than 90%, and the compounds were protected against degradation during storage (90 days). The nanoparticle formulations containing the botanical compounds also showed less toxicity in the tests performed with a biomarker (Artemia salina). In addition, the systems showed bactericidal activity against the important fish pathogenic bacteria Aeromonas hydrophila, Edwardsiella tarda, and Streptococcus iniae in vitro. The present study opens new perspectives for the use of botanical compounds in combination with nanotechnology to treat fish diseases caused by bacteria, contributing to a more sustainable fish chain production. |
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Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish PathogensThe supply of food derived from aquaculture has increased significantly in recent years. The aim of this industrial sector is to produce sustainable products to meet the needs of consumers, providing food security and nutritional benefits. The development of aquaculture has faced challenges including disease outbreaks that can cause substantial economic losses. These diseases can be controlled using chemicals such as antibiotics. However, the indiscriminate use of these substances can have major negative impacts on human health and the environment with the additional risk of the emergence of resistant organisms. The present manuscript describes the use of phytotherapy in association with nanotechnology in order to obtain a more effective and less harmful system for the control of bacterial diseases in fish. Zein nanoparticles associated with eugenol and garlic essential oil were prepared through antisolvent precipitation and characterized. Zein nanoparticles are promising carrier systems as zein proteins are biodegradable and biocompatible and, in this way, good candidates for encapsulation of active ingredients. The system presented good physicochemical properties with an average particle diameter of approximately 150 nm, a polydispersity index lower than 0.2, and a zeta potential of approximately 30 mV. High encapsulation efficiency was obtained for the active compounds with values higher than 90%, and the compounds were protected against degradation during storage (90 days). The nanoparticle formulations containing the botanical compounds also showed less toxicity in the tests performed with a biomarker (Artemia salina). In addition, the systems showed bactericidal activity against the important fish pathogenic bacteria Aeromonas hydrophila, Edwardsiella tarda, and Streptococcus iniae in vitro. The present study opens new perspectives for the use of botanical compounds in combination with nanotechnology to treat fish diseases caused by bacteria, contributing to a more sustainable fish chain production.Institute of Science and Technology Laboratory of Environmental Nanotechnology São Paulo State University (UNESP)Federal University of ABCLaboratory of Bioactivity Assessment and Toxicology of Nanomaterials (LABiToN) University of SorocabaLaboratory of Ecotoxicology and Biosafety Embrapa EnvironmentInstitute of Science and Technology Laboratory of Environmental Nanotechnology São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Federal University of ABCUniversity of SorocabaEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Luis, Angélica I. S. [UNESP]Campos, Estefânia V. R.De Oliveira, Jhones L. [UNESP]Guilger-Casagrande, MarianaDe Lima, RenataCastanha, Rodrigo F.De Castro, Vera L. S. S.Fraceto, Leonardo F. [UNESP]2020-12-12T02:44:47Z2020-12-12T02:44:47Z2020-06-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15557-15566application/pdfhttp://dx.doi.org/10.1021/acsomega.0c01716ACS Omega, v. 5, n. 25, p. 15557-15566, 2020.2470-1343http://hdl.handle.net/11449/20190010.1021/acsomega.0c017162-s2.0-85086873881Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengACS Omegainfo:eu-repo/semantics/openAccess2023-11-10T06:10:23Zoai:repositorio.unesp.br:11449/201900Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:17:53.981945Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens |
title |
Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens |
spellingShingle |
Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens Luis, Angélica I. S. [UNESP] |
title_short |
Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens |
title_full |
Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens |
title_fullStr |
Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens |
title_full_unstemmed |
Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens |
title_sort |
Zein Nanoparticles Impregnated with Eugenol and Garlic Essential Oils for Treating Fish Pathogens |
author |
Luis, Angélica I. S. [UNESP] |
author_facet |
Luis, Angélica I. S. [UNESP] Campos, Estefânia V. R. De Oliveira, Jhones L. [UNESP] Guilger-Casagrande, Mariana De Lima, Renata Castanha, Rodrigo F. De Castro, Vera L. S. S. Fraceto, Leonardo F. [UNESP] |
author_role |
author |
author2 |
Campos, Estefânia V. R. De Oliveira, Jhones L. [UNESP] Guilger-Casagrande, Mariana De Lima, Renata Castanha, Rodrigo F. De Castro, Vera L. S. S. Fraceto, Leonardo F. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of ABC University of Sorocaba Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Luis, Angélica I. S. [UNESP] Campos, Estefânia V. R. De Oliveira, Jhones L. [UNESP] Guilger-Casagrande, Mariana De Lima, Renata Castanha, Rodrigo F. De Castro, Vera L. S. S. Fraceto, Leonardo F. [UNESP] |
description |
The supply of food derived from aquaculture has increased significantly in recent years. The aim of this industrial sector is to produce sustainable products to meet the needs of consumers, providing food security and nutritional benefits. The development of aquaculture has faced challenges including disease outbreaks that can cause substantial economic losses. These diseases can be controlled using chemicals such as antibiotics. However, the indiscriminate use of these substances can have major negative impacts on human health and the environment with the additional risk of the emergence of resistant organisms. The present manuscript describes the use of phytotherapy in association with nanotechnology in order to obtain a more effective and less harmful system for the control of bacterial diseases in fish. Zein nanoparticles associated with eugenol and garlic essential oil were prepared through antisolvent precipitation and characterized. Zein nanoparticles are promising carrier systems as zein proteins are biodegradable and biocompatible and, in this way, good candidates for encapsulation of active ingredients. The system presented good physicochemical properties with an average particle diameter of approximately 150 nm, a polydispersity index lower than 0.2, and a zeta potential of approximately 30 mV. High encapsulation efficiency was obtained for the active compounds with values higher than 90%, and the compounds were protected against degradation during storage (90 days). The nanoparticle formulations containing the botanical compounds also showed less toxicity in the tests performed with a biomarker (Artemia salina). In addition, the systems showed bactericidal activity against the important fish pathogenic bacteria Aeromonas hydrophila, Edwardsiella tarda, and Streptococcus iniae in vitro. The present study opens new perspectives for the use of botanical compounds in combination with nanotechnology to treat fish diseases caused by bacteria, contributing to a more sustainable fish chain production. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:44:47Z 2020-12-12T02:44:47Z 2020-06-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acsomega.0c01716 ACS Omega, v. 5, n. 25, p. 15557-15566, 2020. 2470-1343 http://hdl.handle.net/11449/201900 10.1021/acsomega.0c01716 2-s2.0-85086873881 |
url |
http://dx.doi.org/10.1021/acsomega.0c01716 http://hdl.handle.net/11449/201900 |
identifier_str_mv |
ACS Omega, v. 5, n. 25, p. 15557-15566, 2020. 2470-1343 10.1021/acsomega.0c01716 2-s2.0-85086873881 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
ACS Omega |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
15557-15566 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128787114622976 |