Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.prevetmed.2021.105316 http://hdl.handle.net/11449/207432 |
Resumo: | Faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli (ESC-R E. coli) in dogs has been reported worldwide and can reduce the effectiveness of treatments against bacterial infections. However, the drivers that influence faecal carriage of ESC-R E. coli in dogs are poorly understood. The aims of this study were to estimate the prevalence of ESC-R E. coli among dogs prior to their admission to a veterinary teaching hospital and to identify risk factors associated with the faecal carriage of ESC-R E. coli. Rectal swabs (n = 130) were collected from dogs and screened for ESC-R E. coli using MacConkey agar supplemented with cefotaxime (2 μg/mL). E. coli species was confirmed by MALDI-TOF and screening of extended-spectrum beta-lactamase (ESBL) genes was conducted by multiplex PCR. Questionnaires were completed by each dog's owner to test several human and dog characteristics associated with ESC-R E. coli. The prevalence of faecal carriage of ESC-R E. coli was 9.2 % and 67 % of ESC-R E. coli isolates harboured ESBL genes including CTX-M alone or in combination with TEM. All ESC-R E. coli isolates were resistant to ceftriaxone, cefpodoxime, and cefotaxime and were susceptible to cefoxitin and carbapenems. The likelihood of carrying ESC-R E. coli was 15 times higher (OR = 14.41 [95 % CI: 1.80−38.02], p < 0.01) if the dog was treated with antibiotics 3–12 months prior to sampling and 8 times higher (OR = 7.96 [95 % CI: 2.96−92.07], p < 0.01) if the dog had direct contact with livestock, but 15 times lower (OR = 0.07 [95 % CI: 0.01−0.32], p < 0.01) if the dog was dewormed during the previous year. Our findings confirm the faecal carriage of ESC-R E. coli in subclinical dogs and call for further investigation regarding the impact of deworming on antibiotic-resistant bacteria in companion animals. |
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Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast BrazilAntimicrobial resistanceCompanion animalsDewormingE. coliExtended-spectrum beta-lactamaseLatin AmericaFaecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli (ESC-R E. coli) in dogs has been reported worldwide and can reduce the effectiveness of treatments against bacterial infections. However, the drivers that influence faecal carriage of ESC-R E. coli in dogs are poorly understood. The aims of this study were to estimate the prevalence of ESC-R E. coli among dogs prior to their admission to a veterinary teaching hospital and to identify risk factors associated with the faecal carriage of ESC-R E. coli. Rectal swabs (n = 130) were collected from dogs and screened for ESC-R E. coli using MacConkey agar supplemented with cefotaxime (2 μg/mL). E. coli species was confirmed by MALDI-TOF and screening of extended-spectrum beta-lactamase (ESBL) genes was conducted by multiplex PCR. Questionnaires were completed by each dog's owner to test several human and dog characteristics associated with ESC-R E. coli. The prevalence of faecal carriage of ESC-R E. coli was 9.2 % and 67 % of ESC-R E. coli isolates harboured ESBL genes including CTX-M alone or in combination with TEM. All ESC-R E. coli isolates were resistant to ceftriaxone, cefpodoxime, and cefotaxime and were susceptible to cefoxitin and carbapenems. The likelihood of carrying ESC-R E. coli was 15 times higher (OR = 14.41 [95 % CI: 1.80−38.02], p < 0.01) if the dog was treated with antibiotics 3–12 months prior to sampling and 8 times higher (OR = 7.96 [95 % CI: 2.96−92.07], p < 0.01) if the dog had direct contact with livestock, but 15 times lower (OR = 0.07 [95 % CI: 0.01−0.32], p < 0.01) if the dog was dewormed during the previous year. Our findings confirm the faecal carriage of ESC-R E. coli in subclinical dogs and call for further investigation regarding the impact of deworming on antibiotic-resistant bacteria in companion animals.Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science Sao Paulo State University (UNESP)Millennium Initiative for Collaborative Research On Bacterial Resistance (MICROB-R)Departamento de Ecología y Biodiversidad Facultad de Ciencias de la Vida Universidad Andres BelloCentro de Investigación para la Sustentabilidad Universidad Andres Bello, Facultad de Ciencias de la VidaGenomics and Resistant Microbes Group Facultad de Medicina Clínica Alemana Universidad del DesarrolloEscuela de Medicina Facultad de Medicina Pontificia Universidad Católica de ChileEscuela de Medicina Veterinaria Pontificia Universidad Católica de ChileDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science Sao Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Millennium Initiative for Collaborative Research On Bacterial Resistance (MICROB-R)Universidad Andres BelloUniversidad del DesarrolloPontificia Universidad Católica de ChileSalgado-Caxito, Marília [UNESP]Benavides, Julio A.Munita, Jose M.Rivas, LinaGarcía, PatriciaListoni, Fernando J.P. [UNESP]Moreno-Switt, Andrea I.Paes, Antonio C. [UNESP]2021-06-25T10:55:03Z2021-06-25T10:55:03Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.prevetmed.2021.105316Preventive Veterinary Medicine, v. 190.0167-5877http://hdl.handle.net/11449/20743210.1016/j.prevetmed.2021.1053162-s2.0-85102389846Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPreventive Veterinary Medicineinfo:eu-repo/semantics/openAccess2024-09-09T13:01:23Zoai:repositorio.unesp.br:11449/207432Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T13:01:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast Brazil |
title |
Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast Brazil |
spellingShingle |
Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast Brazil Salgado-Caxito, Marília [UNESP] Antimicrobial resistance Companion animals Deworming E. coli Extended-spectrum beta-lactamase Latin America |
title_short |
Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast Brazil |
title_full |
Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast Brazil |
title_fullStr |
Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast Brazil |
title_full_unstemmed |
Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast Brazil |
title_sort |
Risk factors associated with faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli among dogs in Southeast Brazil |
author |
Salgado-Caxito, Marília [UNESP] |
author_facet |
Salgado-Caxito, Marília [UNESP] Benavides, Julio A. Munita, Jose M. Rivas, Lina García, Patricia Listoni, Fernando J.P. [UNESP] Moreno-Switt, Andrea I. Paes, Antonio C. [UNESP] |
author_role |
author |
author2 |
Benavides, Julio A. Munita, Jose M. Rivas, Lina García, Patricia Listoni, Fernando J.P. [UNESP] Moreno-Switt, Andrea I. Paes, Antonio C. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Millennium Initiative for Collaborative Research On Bacterial Resistance (MICROB-R) Universidad Andres Bello Universidad del Desarrollo Pontificia Universidad Católica de Chile |
dc.contributor.author.fl_str_mv |
Salgado-Caxito, Marília [UNESP] Benavides, Julio A. Munita, Jose M. Rivas, Lina García, Patricia Listoni, Fernando J.P. [UNESP] Moreno-Switt, Andrea I. Paes, Antonio C. [UNESP] |
dc.subject.por.fl_str_mv |
Antimicrobial resistance Companion animals Deworming E. coli Extended-spectrum beta-lactamase Latin America |
topic |
Antimicrobial resistance Companion animals Deworming E. coli Extended-spectrum beta-lactamase Latin America |
description |
Faecal carriage of extended-spectrum cephalosporin-resistant Escherichia coli (ESC-R E. coli) in dogs has been reported worldwide and can reduce the effectiveness of treatments against bacterial infections. However, the drivers that influence faecal carriage of ESC-R E. coli in dogs are poorly understood. The aims of this study were to estimate the prevalence of ESC-R E. coli among dogs prior to their admission to a veterinary teaching hospital and to identify risk factors associated with the faecal carriage of ESC-R E. coli. Rectal swabs (n = 130) were collected from dogs and screened for ESC-R E. coli using MacConkey agar supplemented with cefotaxime (2 μg/mL). E. coli species was confirmed by MALDI-TOF and screening of extended-spectrum beta-lactamase (ESBL) genes was conducted by multiplex PCR. Questionnaires were completed by each dog's owner to test several human and dog characteristics associated with ESC-R E. coli. The prevalence of faecal carriage of ESC-R E. coli was 9.2 % and 67 % of ESC-R E. coli isolates harboured ESBL genes including CTX-M alone or in combination with TEM. All ESC-R E. coli isolates were resistant to ceftriaxone, cefpodoxime, and cefotaxime and were susceptible to cefoxitin and carbapenems. The likelihood of carrying ESC-R E. coli was 15 times higher (OR = 14.41 [95 % CI: 1.80−38.02], p < 0.01) if the dog was treated with antibiotics 3–12 months prior to sampling and 8 times higher (OR = 7.96 [95 % CI: 2.96−92.07], p < 0.01) if the dog had direct contact with livestock, but 15 times lower (OR = 0.07 [95 % CI: 0.01−0.32], p < 0.01) if the dog was dewormed during the previous year. Our findings confirm the faecal carriage of ESC-R E. coli in subclinical dogs and call for further investigation regarding the impact of deworming on antibiotic-resistant bacteria in companion animals. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:55:03Z 2021-06-25T10:55:03Z 2021-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.prevetmed.2021.105316 Preventive Veterinary Medicine, v. 190. 0167-5877 http://hdl.handle.net/11449/207432 10.1016/j.prevetmed.2021.105316 2-s2.0-85102389846 |
url |
http://dx.doi.org/10.1016/j.prevetmed.2021.105316 http://hdl.handle.net/11449/207432 |
identifier_str_mv |
Preventive Veterinary Medicine, v. 190. 0167-5877 10.1016/j.prevetmed.2021.105316 2-s2.0-85102389846 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Preventive Veterinary Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021332458405888 |