Diferenciação gonadal em Piaractus mesopotamicus

Detalhes bibliográficos
Autor(a) principal: Barbosa, Roosevelt Passos [UNESP]
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/131955
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-12-2015/000854852.pdf
Resumo: In this study, aspects related with growth performance and characterization of the germ cells from diploids (2n) and triploids (3n) males and females of yellow tail tetra Astyanax altiparanae were analyzed. The principal aim was to verify the performance and sterility of 3n fish. In the Chapter I, growth and carcass traits were examined. Female 3n presented high values of saturated fatty acids in comparison with unsaturated and polyunsaturated. Additionally, decreased GSI and better growth performance was verified within triploids. Triploid males presented similar GSI and similar performance parameters in comparison with normal diploid males. In the Chapter II, the aim was to study histologically the gonads of diploids and triploids, and additionally to characterize the spines in the pelvic and anal fins (sexual secondary trait in male), and some sperm parameters (motility, concentration and viability, ultrastructure and ploidy) of 2n and 3n fishes. Triploid males presented spines in the anal fin, similarly as observed in diploids. Histological analysis showed that 2n female present developed gonads with vitelogenic oocytes. On the other hand, 3n female exhibit immature gonads. The gonads of 2n and 3n males presented similar morphology, however, 2n male presented increased amount of spermatozoa. Triploid males also presented decreased sperm concentration, motility and duration of motility. Regarding the sperm viability, no significant differences were observed (P > 0.05). In both statuses, the spermatozoa presented similar ultrastructures and same number of mitochondria, with characteristic morphological structures: head (round), midpiece and flagela, which have a typical tubular formation (9+2). Additionally, the flow cytometrycal analysis showed that 3n male produce aneuploid spermatozoa (n=1.71). In conclusion, the triploidization does not ensure sterility in males, however, 3n female (sterile) may be used for aquaculture ...
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spelling Diferenciação gonadal em Piaractus mesopotamicusPeixePacu (Peixe)Sexo (Biologia)HistologiaGonadasHistologyIn this study, aspects related with growth performance and characterization of the germ cells from diploids (2n) and triploids (3n) males and females of yellow tail tetra Astyanax altiparanae were analyzed. The principal aim was to verify the performance and sterility of 3n fish. In the Chapter I, growth and carcass traits were examined. Female 3n presented high values of saturated fatty acids in comparison with unsaturated and polyunsaturated. Additionally, decreased GSI and better growth performance was verified within triploids. Triploid males presented similar GSI and similar performance parameters in comparison with normal diploid males. In the Chapter II, the aim was to study histologically the gonads of diploids and triploids, and additionally to characterize the spines in the pelvic and anal fins (sexual secondary trait in male), and some sperm parameters (motility, concentration and viability, ultrastructure and ploidy) of 2n and 3n fishes. Triploid males presented spines in the anal fin, similarly as observed in diploids. Histological analysis showed that 2n female present developed gonads with vitelogenic oocytes. On the other hand, 3n female exhibit immature gonads. The gonads of 2n and 3n males presented similar morphology, however, 2n male presented increased amount of spermatozoa. Triploid males also presented decreased sperm concentration, motility and duration of motility. Regarding the sperm viability, no significant differences were observed (P > 0.05). In both statuses, the spermatozoa presented similar ultrastructures and same number of mitochondria, with characteristic morphological structures: head (round), midpiece and flagela, which have a typical tubular formation (9+2). Additionally, the flow cytometrycal analysis showed that 3n male produce aneuploid spermatozoa (n=1.71). In conclusion, the triploidization does not ensure sterility in males, however, 3n female (sterile) may be used for aquaculture ...Neste estudo, foram avaliados aspectos relacionados ao desempenho zootécnico e caracterização das células germinativas de machos e fêmeas diploides (2n) e triploides (3n) de Astyanax altiparanae. O principal objetivo foi verificar a performance e a possível esterilidade em peixes 3n. No Capítulo I, o crescimento e as características da carcaça foram examinadas. Fêmeas 3n apresentam maiores valores de ácidos graxos saturados e menores de insaturados e poli-insaturados. Adicionalmente, foi verificada uma diminuição no IGS e maior crescimento. Machos triploides apresentaram IGS e crescimento similares em comparação com os machos diploides normais. No Capítulo II o objetivo foi analisar histologicamente as gônadas dos diploides e triploides, e adicionalmente, caracterizar a estrutura das espículas presentes nas nadadeiras pélvicas e anal (característica sexual secundária em machos) e alguns parâmetros seminais (motilidade, concentração e viabilidade, ultraestrutura e ploidia). Machos 3n apresentam espículas nas nadadeiras pélvicas e anal, assim como observado nos diploides. Análise histológica mostrou que fêmeas 2n apresentaram gônadas desenvolvidas com ovócitos vitelogênicos. Por outro lado, fêmeas 3n exibiram gônadas imaturas. As gônadas dos machos 2n e 3n apresentaram morfologia similar, no entanto, machos 2n exibiram maior quantidade espermatozoides. Machos 3n apresentaram menor concentração espermática, motilidade e duração da motilidade. No que se refere à viabilidade espermática, não foi observada diferença significativa (P > 0,05). Em ambas ploidias, os espermatozoides apresentaram ultraestrutura similar e mesmo número de mitocôndrias, com estruturas morfológicas características: cabeça (redonda), peça e flagelo, este com formação tubular típica (9+2). Adicionalmente, a análise de citometria de fluxo mostrou que machos triploides produzem espermatozoides aneuploides (n=1,71). Em conclusão.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CNPq: 131296/2013-2Universidade Estadual Paulista (Unesp)Batlouni, Sergio Ricardo [UNESP]Kuradomi, Rafael Yutaka [UNESP]Universidade Estadual Paulista (Unesp)Barbosa, Roosevelt Passos [UNESP]2015-12-10T14:22:46Z2015-12-10T14:22:46Z2015-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisviii, 42 f. : il.application/pdfBARBOSA, Roosevelt Passos. Diferenciação gonadal em Piaractus mesopotamicus. 2015. viii, 42 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Centro de Aquicultura de Jaboticabal, 2015.http://hdl.handle.net/11449/131955000854852http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-12-2015/000854852.pdf33004102049P7Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-06-05T12:40:05Zoai:repositorio.unesp.br:11449/131955Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:00:36.585976Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Diferenciação gonadal em Piaractus mesopotamicus
title Diferenciação gonadal em Piaractus mesopotamicus
spellingShingle Diferenciação gonadal em Piaractus mesopotamicus
Barbosa, Roosevelt Passos [UNESP]
Peixe
Pacu (Peixe)
Sexo (Biologia)
Histologia
Gonadas
Histology
title_short Diferenciação gonadal em Piaractus mesopotamicus
title_full Diferenciação gonadal em Piaractus mesopotamicus
title_fullStr Diferenciação gonadal em Piaractus mesopotamicus
title_full_unstemmed Diferenciação gonadal em Piaractus mesopotamicus
title_sort Diferenciação gonadal em Piaractus mesopotamicus
author Barbosa, Roosevelt Passos [UNESP]
author_facet Barbosa, Roosevelt Passos [UNESP]
author_role author
dc.contributor.none.fl_str_mv Batlouni, Sergio Ricardo [UNESP]
Kuradomi, Rafael Yutaka [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Barbosa, Roosevelt Passos [UNESP]
dc.subject.por.fl_str_mv Peixe
Pacu (Peixe)
Sexo (Biologia)
Histologia
Gonadas
Histology
topic Peixe
Pacu (Peixe)
Sexo (Biologia)
Histologia
Gonadas
Histology
description In this study, aspects related with growth performance and characterization of the germ cells from diploids (2n) and triploids (3n) males and females of yellow tail tetra Astyanax altiparanae were analyzed. The principal aim was to verify the performance and sterility of 3n fish. In the Chapter I, growth and carcass traits were examined. Female 3n presented high values of saturated fatty acids in comparison with unsaturated and polyunsaturated. Additionally, decreased GSI and better growth performance was verified within triploids. Triploid males presented similar GSI and similar performance parameters in comparison with normal diploid males. In the Chapter II, the aim was to study histologically the gonads of diploids and triploids, and additionally to characterize the spines in the pelvic and anal fins (sexual secondary trait in male), and some sperm parameters (motility, concentration and viability, ultrastructure and ploidy) of 2n and 3n fishes. Triploid males presented spines in the anal fin, similarly as observed in diploids. Histological analysis showed that 2n female present developed gonads with vitelogenic oocytes. On the other hand, 3n female exhibit immature gonads. The gonads of 2n and 3n males presented similar morphology, however, 2n male presented increased amount of spermatozoa. Triploid males also presented decreased sperm concentration, motility and duration of motility. Regarding the sperm viability, no significant differences were observed (P > 0.05). In both statuses, the spermatozoa presented similar ultrastructures and same number of mitochondria, with characteristic morphological structures: head (round), midpiece and flagela, which have a typical tubular formation (9+2). Additionally, the flow cytometrycal analysis showed that 3n male produce aneuploid spermatozoa (n=1.71). In conclusion, the triploidization does not ensure sterility in males, however, 3n female (sterile) may be used for aquaculture ...
publishDate 2015
dc.date.none.fl_str_mv 2015-12-10T14:22:46Z
2015-12-10T14:22:46Z
2015-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv BARBOSA, Roosevelt Passos. Diferenciação gonadal em Piaractus mesopotamicus. 2015. viii, 42 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Centro de Aquicultura de Jaboticabal, 2015.
http://hdl.handle.net/11449/131955
000854852
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-12-2015/000854852.pdf
33004102049P7
identifier_str_mv BARBOSA, Roosevelt Passos. Diferenciação gonadal em Piaractus mesopotamicus. 2015. viii, 42 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Centro de Aquicultura de Jaboticabal, 2015.
000854852
33004102049P7
url http://hdl.handle.net/11449/131955
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/09-12-2015/000854852.pdf
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv viii, 42 f. : il.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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