In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3382/japr/pfx045 http://hdl.handle.net/11449/175985 |
Resumo: | The health of poultry is closely related to how the animals are raised, and the gut microbiota plays a key role in the fulfillment of their productive potential. Lactobacillus spp. are bacteria present in the natural microbiota of poultry that, when employed as probiotic agents, should present several characteristics. This study aims at performing the in vitro probiotic isolation and characterization of Lactobacillus spp. Isolates were obtained from ceca content of healthy turkeys through the isolation and identification of morphological, molecular, and physiologic characteristics. They were identified through Gram staining, catalase and hydrogen peroxide production tests, gas production tests during glucose fermentation, and hydrogen sulfide production during the triple sugar iron test. The samples were identified molecularly through polymerase chain reaction tests and were subjected to genetic sequencing. The assessment of the probiotic potential was conducted through artificial gastric juice tolerance and bile salt tolerance tests. A hydrophobicity test was used as an indirect method of assessing an isolate's probable ability to adhere to the intestinal mucosa. In addition, other analyses were performed, such as multiplication potential tests, Salmonella Heildelberg antagonism assays, hydrogen peroxide production tests, and antibiograms, as well as an assessment of the genes responsible for resistance to antimicrobial agents in Integron C. In conclusion, we identified, through morphologic, physiologic, pathogen antagonism, and antimicrobial resistance tests, 11 strains of Lactobacillus spp. belonging to species L. reuteri (9), L. johnsonii (1), and L. frumenti (1) that showed potential as probiotic candidates for in vivo application. |
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In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated TurkeysMicrobiotaPathogenPoultryProbioticResistanceThe health of poultry is closely related to how the animals are raised, and the gut microbiota plays a key role in the fulfillment of their productive potential. Lactobacillus spp. are bacteria present in the natural microbiota of poultry that, when employed as probiotic agents, should present several characteristics. This study aims at performing the in vitro probiotic isolation and characterization of Lactobacillus spp. Isolates were obtained from ceca content of healthy turkeys through the isolation and identification of morphological, molecular, and physiologic characteristics. They were identified through Gram staining, catalase and hydrogen peroxide production tests, gas production tests during glucose fermentation, and hydrogen sulfide production during the triple sugar iron test. The samples were identified molecularly through polymerase chain reaction tests and were subjected to genetic sequencing. The assessment of the probiotic potential was conducted through artificial gastric juice tolerance and bile salt tolerance tests. A hydrophobicity test was used as an indirect method of assessing an isolate's probable ability to adhere to the intestinal mucosa. In addition, other analyses were performed, such as multiplication potential tests, Salmonella Heildelberg antagonism assays, hydrogen peroxide production tests, and antibiograms, as well as an assessment of the genes responsible for resistance to antimicrobial agents in Integron C. In conclusion, we identified, through morphologic, physiologic, pathogen antagonism, and antimicrobial resistance tests, 11 strains of Lactobacillus spp. belonging to species L. reuteri (9), L. johnsonii (1), and L. frumenti (1) that showed potential as probiotic candidates for in vivo application.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Veterinary Clinic Department FMVZ UNESP Botucatu - SPBiostatistic Department IBB UNESP Botucatu - SPVeterinary Clinic Department FMVZ UNESP Botucatu - SPBiostatistic Department IBB UNESP Botucatu - SPUniversidade Estadual Paulista (Unesp)Altarugio, R. [UNESP]Vellano, I. H.B. [UNESP]Moraes, A. C.I. [UNESP]Milbradt, E. L. [UNESP]Filho, R.L. Andreatti [UNESP]Guimarães-Okamoto, P. T.C. [UNESP]Padovani, C. R. [UNESP]Okamoto, A. S. [UNESP]2018-12-11T17:18:25Z2018-12-11T17:18:25Z2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article81-91application/pdfhttp://dx.doi.org/10.3382/japr/pfx045Journal of Applied Poultry Research, v. 27, n. 1, p. 81-91, 2018.1537-04371056-6171http://hdl.handle.net/11449/17598510.3382/japr/pfx0452-s2.0-850432299892-s2.0-85043229989.pdf4703983380224373508548498021412585024628735174640000-0002-0355-98410000-0003-1944-24260000-0003-4939-8024Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Poultry Research0,5850,585info:eu-repo/semantics/openAccess2023-10-18T06:10:31Zoai:repositorio.unesp.br:11449/175985Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:15:54.608246Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys |
title |
In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys |
spellingShingle |
In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys Altarugio, R. [UNESP] Microbiota Pathogen Poultry Probiotic Resistance |
title_short |
In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys |
title_full |
In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys |
title_fullStr |
In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys |
title_full_unstemmed |
In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys |
title_sort |
In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys |
author |
Altarugio, R. [UNESP] |
author_facet |
Altarugio, R. [UNESP] Vellano, I. H.B. [UNESP] Moraes, A. C.I. [UNESP] Milbradt, E. L. [UNESP] Filho, R.L. Andreatti [UNESP] Guimarães-Okamoto, P. T.C. [UNESP] Padovani, C. R. [UNESP] Okamoto, A. S. [UNESP] |
author_role |
author |
author2 |
Vellano, I. H.B. [UNESP] Moraes, A. C.I. [UNESP] Milbradt, E. L. [UNESP] Filho, R.L. Andreatti [UNESP] Guimarães-Okamoto, P. T.C. [UNESP] Padovani, C. R. [UNESP] Okamoto, A. S. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Altarugio, R. [UNESP] Vellano, I. H.B. [UNESP] Moraes, A. C.I. [UNESP] Milbradt, E. L. [UNESP] Filho, R.L. Andreatti [UNESP] Guimarães-Okamoto, P. T.C. [UNESP] Padovani, C. R. [UNESP] Okamoto, A. S. [UNESP] |
dc.subject.por.fl_str_mv |
Microbiota Pathogen Poultry Probiotic Resistance |
topic |
Microbiota Pathogen Poultry Probiotic Resistance |
description |
The health of poultry is closely related to how the animals are raised, and the gut microbiota plays a key role in the fulfillment of their productive potential. Lactobacillus spp. are bacteria present in the natural microbiota of poultry that, when employed as probiotic agents, should present several characteristics. This study aims at performing the in vitro probiotic isolation and characterization of Lactobacillus spp. Isolates were obtained from ceca content of healthy turkeys through the isolation and identification of morphological, molecular, and physiologic characteristics. They were identified through Gram staining, catalase and hydrogen peroxide production tests, gas production tests during glucose fermentation, and hydrogen sulfide production during the triple sugar iron test. The samples were identified molecularly through polymerase chain reaction tests and were subjected to genetic sequencing. The assessment of the probiotic potential was conducted through artificial gastric juice tolerance and bile salt tolerance tests. A hydrophobicity test was used as an indirect method of assessing an isolate's probable ability to adhere to the intestinal mucosa. In addition, other analyses were performed, such as multiplication potential tests, Salmonella Heildelberg antagonism assays, hydrogen peroxide production tests, and antibiograms, as well as an assessment of the genes responsible for resistance to antimicrobial agents in Integron C. In conclusion, we identified, through morphologic, physiologic, pathogen antagonism, and antimicrobial resistance tests, 11 strains of Lactobacillus spp. belonging to species L. reuteri (9), L. johnsonii (1), and L. frumenti (1) that showed potential as probiotic candidates for in vivo application. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:18:25Z 2018-12-11T17:18:25Z 2018-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3382/japr/pfx045 Journal of Applied Poultry Research, v. 27, n. 1, p. 81-91, 2018. 1537-0437 1056-6171 http://hdl.handle.net/11449/175985 10.3382/japr/pfx045 2-s2.0-85043229989 2-s2.0-85043229989.pdf 4703983380224373 5085484980214125 8502462873517464 0000-0002-0355-9841 0000-0003-1944-2426 0000-0003-4939-8024 |
url |
http://dx.doi.org/10.3382/japr/pfx045 http://hdl.handle.net/11449/175985 |
identifier_str_mv |
Journal of Applied Poultry Research, v. 27, n. 1, p. 81-91, 2018. 1537-0437 1056-6171 10.3382/japr/pfx045 2-s2.0-85043229989 2-s2.0-85043229989.pdf 4703983380224373 5085484980214125 8502462873517464 0000-0002-0355-9841 0000-0003-1944-2426 0000-0003-4939-8024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Poultry Research 0,585 0,585 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
81-91 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128490710499328 |