Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects

Detalhes bibliográficos
Autor(a) principal: Graton, Murilo E. [UNESP]
Data de Publicação: 2022
Outros Autores: Ferreira, Bruno H. S. H. [UNESP], Troiano, Jéssica A. [UNESP], Potje, Simone R., Vale, Gabriel T., Nakamune, Ana Cláudia M. S. [UNESP], Tirapelli, Carlos R., Miller, Francis J., Ximenes, Valdecir F. [UNESP], Antoniali, Cristina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fphys.2022.1047916
http://hdl.handle.net/11449/249010
Resumo: Reactive oxygen species (ROS) derived from NOX enzymes activity play an important role in the development of cardiovascular diseases. Compounds able to decrease oxidative stress damage are potential candidates as drugs and/or supplements for hypertension treatment. Here, we aimed to compare in vitro ROS scavenging potency, effective NOX inhibition and effects on vascular reactivity of apocynin to another phenolic compound, protocatechuic acid, in vascular cells from spontaneously hypertensive rat (SHR), where redox signaling is altered and contributes to the development and/or maintenance of hypertension. We evaluated the in vitro antioxidant capacity and free radical scavenging capacity of both phenolic compounds. Moreover, we investigated the effect of both compounds on lipid peroxidation, lucigenin chemiluminescence, nitric oxide (NO•) levels and ROS concentration in vascular cells of SHR or human umbilical vein endothelial cell (HUVEC). Apocynin and protocatechuic acid presented antioxidant capacity and ability as free radical scavengers, decreased thiobarbituric acid reactive substances (TBARS) in aortic cells from SHR, and increased NO• concentration in isolated HUVEC. Both compounds were able to reduce lucigenin chemiluminescence and increased the potency of acetylcholine in aorta of SHR. However, in SHR aortas, only apocynin diminished the contraction induced by phenylephrine. In conclusion, these results strongly reinforce the potential application of substances such as apocynin and protocatechuic acid that combine abilities as scavenging and/or prevention of ROS generation, establishment of NO bioactivity and modulation of vascular reactivity. Due to its phytochemical origin and low toxicity, its potential therapeutic use in vascular diseases should be considered.
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spelling Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effectsapocyninnitric oxideoxidative stressprotocatechuic acidreactive oxygen speciesReactive oxygen species (ROS) derived from NOX enzymes activity play an important role in the development of cardiovascular diseases. Compounds able to decrease oxidative stress damage are potential candidates as drugs and/or supplements for hypertension treatment. Here, we aimed to compare in vitro ROS scavenging potency, effective NOX inhibition and effects on vascular reactivity of apocynin to another phenolic compound, protocatechuic acid, in vascular cells from spontaneously hypertensive rat (SHR), where redox signaling is altered and contributes to the development and/or maintenance of hypertension. We evaluated the in vitro antioxidant capacity and free radical scavenging capacity of both phenolic compounds. Moreover, we investigated the effect of both compounds on lipid peroxidation, lucigenin chemiluminescence, nitric oxide (NO•) levels and ROS concentration in vascular cells of SHR or human umbilical vein endothelial cell (HUVEC). Apocynin and protocatechuic acid presented antioxidant capacity and ability as free radical scavengers, decreased thiobarbituric acid reactive substances (TBARS) in aortic cells from SHR, and increased NO• concentration in isolated HUVEC. Both compounds were able to reduce lucigenin chemiluminescence and increased the potency of acetylcholine in aorta of SHR. However, in SHR aortas, only apocynin diminished the contraction induced by phenylephrine. In conclusion, these results strongly reinforce the potential application of substances such as apocynin and protocatechuic acid that combine abilities as scavenging and/or prevention of ROS generation, establishment of NO bioactivity and modulation of vascular reactivity. Due to its phytochemical origin and low toxicity, its potential therapeutic use in vascular diseases should be considered.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Institute of Standards and TechnologyPrograma de Pós-graduação Multicêntrico em Ciências Fisiológicas SBFis São Paulo State University (UNESP), São PauloDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP), São PauloDepartment of Support Production and Animal Health School of Veterinary Medicine São Paulo State University (UNESP), São PauloFundação Dracenense de Educação e Cultura (FUNDEC) Faculdades de Dracena (UNIFADRA)Department of Biosciences Minas Gerais State University (UEMG), Minas GeraisDepartment of Psychiatry Nursing and Human Sciences College of Nursing of Ribeirão Preto University of São Paulo (USP), São PauloNashville VA Medical Center Vanderbilt UniversityDepartment of Chemistry Faculty of Sciences São Paulo State University (UNESP), São PauloPrograma de Pós-graduação Multicêntrico em Ciências Fisiológicas SBFis São Paulo State University (UNESP), São PauloDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP), São PauloDepartment of Support Production and Animal Health School of Veterinary Medicine São Paulo State University (UNESP), São PauloDepartment of Chemistry Faculty of Sciences São Paulo State University (UNESP), São PauloFAPESP: 2016/22180-9 2016/01476-7 2017/18436-0CNPq: 302526/2016-1National Institute of Standards and Technology: 465637/2014-0 2014/50926-0Universidade Estadual Paulista (UNESP)Faculdades de Dracena (UNIFADRA)Universidade Estadual de Maringá (UEM)Universidade de São Paulo (USP)Vanderbilt UniversityGraton, Murilo E. [UNESP]Ferreira, Bruno H. S. H. [UNESP]Troiano, Jéssica A. [UNESP]Potje, Simone R.Vale, Gabriel T.Nakamune, Ana Cláudia M. S. [UNESP]Tirapelli, Carlos R.Miller, Francis J.Ximenes, Valdecir F. [UNESP]Antoniali, Cristina [UNESP]2023-07-29T13:59:57Z2023-07-29T13:59:57Z2022-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fphys.2022.1047916Frontiers in Physiology, v. 13.1664-042Xhttp://hdl.handle.net/11449/24901010.3389/fphys.2022.10479162-s2.0-85143160730Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Physiologyinfo:eu-repo/semantics/openAccess2024-08-15T18:46:26Zoai:repositorio.unesp.br:11449/249010Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T18:46:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects
title Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects
spellingShingle Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects
Graton, Murilo E. [UNESP]
apocynin
nitric oxide
oxidative stress
protocatechuic acid
reactive oxygen species
title_short Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects
title_full Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects
title_fullStr Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects
title_full_unstemmed Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects
title_sort Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects
author Graton, Murilo E. [UNESP]
author_facet Graton, Murilo E. [UNESP]
Ferreira, Bruno H. S. H. [UNESP]
Troiano, Jéssica A. [UNESP]
Potje, Simone R.
Vale, Gabriel T.
Nakamune, Ana Cláudia M. S. [UNESP]
Tirapelli, Carlos R.
Miller, Francis J.
Ximenes, Valdecir F. [UNESP]
Antoniali, Cristina [UNESP]
author_role author
author2 Ferreira, Bruno H. S. H. [UNESP]
Troiano, Jéssica A. [UNESP]
Potje, Simone R.
Vale, Gabriel T.
Nakamune, Ana Cláudia M. S. [UNESP]
Tirapelli, Carlos R.
Miller, Francis J.
Ximenes, Valdecir F. [UNESP]
Antoniali, Cristina [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Faculdades de Dracena (UNIFADRA)
Universidade Estadual de Maringá (UEM)
Universidade de São Paulo (USP)
Vanderbilt University
dc.contributor.author.fl_str_mv Graton, Murilo E. [UNESP]
Ferreira, Bruno H. S. H. [UNESP]
Troiano, Jéssica A. [UNESP]
Potje, Simone R.
Vale, Gabriel T.
Nakamune, Ana Cláudia M. S. [UNESP]
Tirapelli, Carlos R.
Miller, Francis J.
Ximenes, Valdecir F. [UNESP]
Antoniali, Cristina [UNESP]
dc.subject.por.fl_str_mv apocynin
nitric oxide
oxidative stress
protocatechuic acid
reactive oxygen species
topic apocynin
nitric oxide
oxidative stress
protocatechuic acid
reactive oxygen species
description Reactive oxygen species (ROS) derived from NOX enzymes activity play an important role in the development of cardiovascular diseases. Compounds able to decrease oxidative stress damage are potential candidates as drugs and/or supplements for hypertension treatment. Here, we aimed to compare in vitro ROS scavenging potency, effective NOX inhibition and effects on vascular reactivity of apocynin to another phenolic compound, protocatechuic acid, in vascular cells from spontaneously hypertensive rat (SHR), where redox signaling is altered and contributes to the development and/or maintenance of hypertension. We evaluated the in vitro antioxidant capacity and free radical scavenging capacity of both phenolic compounds. Moreover, we investigated the effect of both compounds on lipid peroxidation, lucigenin chemiluminescence, nitric oxide (NO•) levels and ROS concentration in vascular cells of SHR or human umbilical vein endothelial cell (HUVEC). Apocynin and protocatechuic acid presented antioxidant capacity and ability as free radical scavengers, decreased thiobarbituric acid reactive substances (TBARS) in aortic cells from SHR, and increased NO• concentration in isolated HUVEC. Both compounds were able to reduce lucigenin chemiluminescence and increased the potency of acetylcholine in aorta of SHR. However, in SHR aortas, only apocynin diminished the contraction induced by phenylephrine. In conclusion, these results strongly reinforce the potential application of substances such as apocynin and protocatechuic acid that combine abilities as scavenging and/or prevention of ROS generation, establishment of NO bioactivity and modulation of vascular reactivity. Due to its phytochemical origin and low toxicity, its potential therapeutic use in vascular diseases should be considered.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-15
2023-07-29T13:59:57Z
2023-07-29T13:59:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fphys.2022.1047916
Frontiers in Physiology, v. 13.
1664-042X
http://hdl.handle.net/11449/249010
10.3389/fphys.2022.1047916
2-s2.0-85143160730
url http://dx.doi.org/10.3389/fphys.2022.1047916
http://hdl.handle.net/11449/249010
identifier_str_mv Frontiers in Physiology, v. 13.
1664-042X
10.3389/fphys.2022.1047916
2-s2.0-85143160730
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Physiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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