Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fphys.2022.1047916 http://hdl.handle.net/11449/249010 |
Resumo: | Reactive oxygen species (ROS) derived from NOX enzymes activity play an important role in the development of cardiovascular diseases. Compounds able to decrease oxidative stress damage are potential candidates as drugs and/or supplements for hypertension treatment. Here, we aimed to compare in vitro ROS scavenging potency, effective NOX inhibition and effects on vascular reactivity of apocynin to another phenolic compound, protocatechuic acid, in vascular cells from spontaneously hypertensive rat (SHR), where redox signaling is altered and contributes to the development and/or maintenance of hypertension. We evaluated the in vitro antioxidant capacity and free radical scavenging capacity of both phenolic compounds. Moreover, we investigated the effect of both compounds on lipid peroxidation, lucigenin chemiluminescence, nitric oxide (NO•) levels and ROS concentration in vascular cells of SHR or human umbilical vein endothelial cell (HUVEC). Apocynin and protocatechuic acid presented antioxidant capacity and ability as free radical scavengers, decreased thiobarbituric acid reactive substances (TBARS) in aortic cells from SHR, and increased NO• concentration in isolated HUVEC. Both compounds were able to reduce lucigenin chemiluminescence and increased the potency of acetylcholine in aorta of SHR. However, in SHR aortas, only apocynin diminished the contraction induced by phenylephrine. In conclusion, these results strongly reinforce the potential application of substances such as apocynin and protocatechuic acid that combine abilities as scavenging and/or prevention of ROS generation, establishment of NO bioactivity and modulation of vascular reactivity. Due to its phytochemical origin and low toxicity, its potential therapeutic use in vascular diseases should be considered. |
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Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effectsapocyninnitric oxideoxidative stressprotocatechuic acidreactive oxygen speciesReactive oxygen species (ROS) derived from NOX enzymes activity play an important role in the development of cardiovascular diseases. Compounds able to decrease oxidative stress damage are potential candidates as drugs and/or supplements for hypertension treatment. Here, we aimed to compare in vitro ROS scavenging potency, effective NOX inhibition and effects on vascular reactivity of apocynin to another phenolic compound, protocatechuic acid, in vascular cells from spontaneously hypertensive rat (SHR), where redox signaling is altered and contributes to the development and/or maintenance of hypertension. We evaluated the in vitro antioxidant capacity and free radical scavenging capacity of both phenolic compounds. Moreover, we investigated the effect of both compounds on lipid peroxidation, lucigenin chemiluminescence, nitric oxide (NO•) levels and ROS concentration in vascular cells of SHR or human umbilical vein endothelial cell (HUVEC). Apocynin and protocatechuic acid presented antioxidant capacity and ability as free radical scavengers, decreased thiobarbituric acid reactive substances (TBARS) in aortic cells from SHR, and increased NO• concentration in isolated HUVEC. Both compounds were able to reduce lucigenin chemiluminescence and increased the potency of acetylcholine in aorta of SHR. However, in SHR aortas, only apocynin diminished the contraction induced by phenylephrine. In conclusion, these results strongly reinforce the potential application of substances such as apocynin and protocatechuic acid that combine abilities as scavenging and/or prevention of ROS generation, establishment of NO bioactivity and modulation of vascular reactivity. Due to its phytochemical origin and low toxicity, its potential therapeutic use in vascular diseases should be considered.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Institute of Standards and TechnologyPrograma de Pós-graduação Multicêntrico em Ciências Fisiológicas SBFis São Paulo State University (UNESP), São PauloDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP), São PauloDepartment of Support Production and Animal Health School of Veterinary Medicine São Paulo State University (UNESP), São PauloFundação Dracenense de Educação e Cultura (FUNDEC) Faculdades de Dracena (UNIFADRA)Department of Biosciences Minas Gerais State University (UEMG), Minas GeraisDepartment of Psychiatry Nursing and Human Sciences College of Nursing of Ribeirão Preto University of São Paulo (USP), São PauloNashville VA Medical Center Vanderbilt UniversityDepartment of Chemistry Faculty of Sciences São Paulo State University (UNESP), São PauloPrograma de Pós-graduação Multicêntrico em Ciências Fisiológicas SBFis São Paulo State University (UNESP), São PauloDepartment of Basic Sciences School of Dentistry São Paulo State University (UNESP), São PauloDepartment of Support Production and Animal Health School of Veterinary Medicine São Paulo State University (UNESP), São PauloDepartment of Chemistry Faculty of Sciences São Paulo State University (UNESP), São PauloFAPESP: 2016/22180-9 2016/01476-7 2017/18436-0CNPq: 302526/2016-1National Institute of Standards and Technology: 465637/2014-0 2014/50926-0Universidade Estadual Paulista (UNESP)Faculdades de Dracena (UNIFADRA)Universidade Estadual de Maringá (UEM)Universidade de São Paulo (USP)Vanderbilt UniversityGraton, Murilo E. [UNESP]Ferreira, Bruno H. S. H. [UNESP]Troiano, Jéssica A. [UNESP]Potje, Simone R.Vale, Gabriel T.Nakamune, Ana Cláudia M. S. [UNESP]Tirapelli, Carlos R.Miller, Francis J.Ximenes, Valdecir F. [UNESP]Antoniali, Cristina [UNESP]2023-07-29T13:59:57Z2023-07-29T13:59:57Z2022-11-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fphys.2022.1047916Frontiers in Physiology, v. 13.1664-042Xhttp://hdl.handle.net/11449/24901010.3389/fphys.2022.10479162-s2.0-85143160730Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Physiologyinfo:eu-repo/semantics/openAccess2024-08-15T18:46:26Zoai:repositorio.unesp.br:11449/249010Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T18:46:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects |
title |
Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects |
spellingShingle |
Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects Graton, Murilo E. [UNESP] apocynin nitric oxide oxidative stress protocatechuic acid reactive oxygen species |
title_short |
Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects |
title_full |
Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects |
title_fullStr |
Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects |
title_full_unstemmed |
Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects |
title_sort |
Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects |
author |
Graton, Murilo E. [UNESP] |
author_facet |
Graton, Murilo E. [UNESP] Ferreira, Bruno H. S. H. [UNESP] Troiano, Jéssica A. [UNESP] Potje, Simone R. Vale, Gabriel T. Nakamune, Ana Cláudia M. S. [UNESP] Tirapelli, Carlos R. Miller, Francis J. Ximenes, Valdecir F. [UNESP] Antoniali, Cristina [UNESP] |
author_role |
author |
author2 |
Ferreira, Bruno H. S. H. [UNESP] Troiano, Jéssica A. [UNESP] Potje, Simone R. Vale, Gabriel T. Nakamune, Ana Cláudia M. S. [UNESP] Tirapelli, Carlos R. Miller, Francis J. Ximenes, Valdecir F. [UNESP] Antoniali, Cristina [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Faculdades de Dracena (UNIFADRA) Universidade Estadual de Maringá (UEM) Universidade de São Paulo (USP) Vanderbilt University |
dc.contributor.author.fl_str_mv |
Graton, Murilo E. [UNESP] Ferreira, Bruno H. S. H. [UNESP] Troiano, Jéssica A. [UNESP] Potje, Simone R. Vale, Gabriel T. Nakamune, Ana Cláudia M. S. [UNESP] Tirapelli, Carlos R. Miller, Francis J. Ximenes, Valdecir F. [UNESP] Antoniali, Cristina [UNESP] |
dc.subject.por.fl_str_mv |
apocynin nitric oxide oxidative stress protocatechuic acid reactive oxygen species |
topic |
apocynin nitric oxide oxidative stress protocatechuic acid reactive oxygen species |
description |
Reactive oxygen species (ROS) derived from NOX enzymes activity play an important role in the development of cardiovascular diseases. Compounds able to decrease oxidative stress damage are potential candidates as drugs and/or supplements for hypertension treatment. Here, we aimed to compare in vitro ROS scavenging potency, effective NOX inhibition and effects on vascular reactivity of apocynin to another phenolic compound, protocatechuic acid, in vascular cells from spontaneously hypertensive rat (SHR), where redox signaling is altered and contributes to the development and/or maintenance of hypertension. We evaluated the in vitro antioxidant capacity and free radical scavenging capacity of both phenolic compounds. Moreover, we investigated the effect of both compounds on lipid peroxidation, lucigenin chemiluminescence, nitric oxide (NO•) levels and ROS concentration in vascular cells of SHR or human umbilical vein endothelial cell (HUVEC). Apocynin and protocatechuic acid presented antioxidant capacity and ability as free radical scavengers, decreased thiobarbituric acid reactive substances (TBARS) in aortic cells from SHR, and increased NO• concentration in isolated HUVEC. Both compounds were able to reduce lucigenin chemiluminescence and increased the potency of acetylcholine in aorta of SHR. However, in SHR aortas, only apocynin diminished the contraction induced by phenylephrine. In conclusion, these results strongly reinforce the potential application of substances such as apocynin and protocatechuic acid that combine abilities as scavenging and/or prevention of ROS generation, establishment of NO bioactivity and modulation of vascular reactivity. Due to its phytochemical origin and low toxicity, its potential therapeutic use in vascular diseases should be considered. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-15 2023-07-29T13:59:57Z 2023-07-29T13:59:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fphys.2022.1047916 Frontiers in Physiology, v. 13. 1664-042X http://hdl.handle.net/11449/249010 10.3389/fphys.2022.1047916 2-s2.0-85143160730 |
url |
http://dx.doi.org/10.3389/fphys.2022.1047916 http://hdl.handle.net/11449/249010 |
identifier_str_mv |
Frontiers in Physiology, v. 13. 1664-042X 10.3389/fphys.2022.1047916 2-s2.0-85143160730 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128117526495232 |