Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas

Detalhes bibliográficos
Autor(a) principal: Sena, Bruna V.
Data de Publicação: 2022
Outros Autores: Horta, Rodrigo S., Senhorello, Igor L.S., Matiz, Oscar R.S., Canavari, Isabela C. [UNESP], Souza, Larissa M., Ferreira, Marília G.P.A., Sueiro, Felipe A.R., Costa, Mirela T. [UNESP], Nardi, Andrigo B. de[UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jcpa.2022.03.001
http://hdl.handle.net/11449/239877
Resumo: The objective of this study was to investigate the immunolabelling of acetylated histones and histone desacetylase (HDAC) enzymes in canine soft tissue sarcomas (STSs) and to correlate them with histological and clinical features in order to identify possible prognostic and therapeutic targets in these neoplasms. Fifteen canine STS samples were evaluated and were submitted to immunohistochemistry for acetylated histones 3 (H3) and 4 (H4) and deacetylating enzymes (HDAC1, HDAC2 and HDAC6). Intense immunolabelling of H4 was seen in comparison with H3. A strong positive correlation was observed between the H3 intensity score and the number of mitotic figures (P = 0.004, r = 0.7). Intense immunolabelling of HDAC1 was found in comparison to the expression of HDAC2 and HDAC6 in the evaluated STSs. This finding suggests that HDAC1 may be a potential target for HDAC inhibitors in STSs in dogs.
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spelling Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomascanineepigenetichistonesmesenchymal neoplasiaThe objective of this study was to investigate the immunolabelling of acetylated histones and histone desacetylase (HDAC) enzymes in canine soft tissue sarcomas (STSs) and to correlate them with histological and clinical features in order to identify possible prognostic and therapeutic targets in these neoplasms. Fifteen canine STS samples were evaluated and were submitted to immunohistochemistry for acetylated histones 3 (H3) and 4 (H4) and deacetylating enzymes (HDAC1, HDAC2 and HDAC6). Intense immunolabelling of H4 was seen in comparison with H3. A strong positive correlation was observed between the H3 intensity score and the number of mitotic figures (P = 0.004, r = 0.7). Intense immunolabelling of HDAC1 was found in comparison to the expression of HDAC2 and HDAC6 in the evaluated STSs. This finding suggests that HDAC1 may be a potential target for HDAC inhibitors in STSs in dogs.Department of Veterinary Medicine and Surgery Veterinay School Universidade Federal de Minas Gerais, MGDepartment of Veterinary Medicine Universidade Vila Velha, ESAutonomous Veterinarian Life – Oncologia VeterináriaDepartment of Clinical and Veterinary Surgery College of Agricultural and Veterinary Sciences Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, SPAutonomous Veterinarian, ESVeterinary Hospital Universidade Federal do Vale do São Francisco HU-Univasf, PEVETPAT Pathology and Molecular Biology Laboratory, SPDepartment of Clinical and Veterinary Surgery College of Agricultural and Veterinary Sciences Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, SPUniversidade Federal de Minas Gerais (UFMG)Universidade Vila VelhaLife – Oncologia VeterináriaUniversidade Estadual Paulista (UNESP)Autonomous VeterinarianHU-UnivasfVETPAT Pathology and Molecular Biology LaboratorySena, Bruna V.Horta, Rodrigo S.Senhorello, Igor L.S.Matiz, Oscar R.S.Canavari, Isabela C. [UNESP]Souza, Larissa M.Ferreira, Marília G.P.A.Sueiro, Felipe A.R.Costa, Mirela T. [UNESP]Nardi, Andrigo B. de[UNESP]2023-03-01T19:51:23Z2023-03-01T19:51:23Z2022-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article51-58http://dx.doi.org/10.1016/j.jcpa.2022.03.001Journal of Comparative Pathology, v. 193, p. 51-58.1532-31290021-9975http://hdl.handle.net/11449/23987710.1016/j.jcpa.2022.03.0012-s2.0-85128184230Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Comparative Pathologyinfo:eu-repo/semantics/openAccess2023-03-01T19:51:23Zoai:repositorio.unesp.br:11449/239877Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:06:05.514906Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas
title Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas
spellingShingle Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas
Sena, Bruna V.
canine
epigenetic
histones
mesenchymal neoplasia
title_short Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas
title_full Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas
title_fullStr Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas
title_full_unstemmed Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas
title_sort Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas
author Sena, Bruna V.
author_facet Sena, Bruna V.
Horta, Rodrigo S.
Senhorello, Igor L.S.
Matiz, Oscar R.S.
Canavari, Isabela C. [UNESP]
Souza, Larissa M.
Ferreira, Marília G.P.A.
Sueiro, Felipe A.R.
Costa, Mirela T. [UNESP]
Nardi, Andrigo B. de[UNESP]
author_role author
author2 Horta, Rodrigo S.
Senhorello, Igor L.S.
Matiz, Oscar R.S.
Canavari, Isabela C. [UNESP]
Souza, Larissa M.
Ferreira, Marília G.P.A.
Sueiro, Felipe A.R.
Costa, Mirela T. [UNESP]
Nardi, Andrigo B. de[UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Minas Gerais (UFMG)
Universidade Vila Velha
Life – Oncologia Veterinária
Universidade Estadual Paulista (UNESP)
Autonomous Veterinarian
HU-Univasf
VETPAT Pathology and Molecular Biology Laboratory
dc.contributor.author.fl_str_mv Sena, Bruna V.
Horta, Rodrigo S.
Senhorello, Igor L.S.
Matiz, Oscar R.S.
Canavari, Isabela C. [UNESP]
Souza, Larissa M.
Ferreira, Marília G.P.A.
Sueiro, Felipe A.R.
Costa, Mirela T. [UNESP]
Nardi, Andrigo B. de[UNESP]
dc.subject.por.fl_str_mv canine
epigenetic
histones
mesenchymal neoplasia
topic canine
epigenetic
histones
mesenchymal neoplasia
description The objective of this study was to investigate the immunolabelling of acetylated histones and histone desacetylase (HDAC) enzymes in canine soft tissue sarcomas (STSs) and to correlate them with histological and clinical features in order to identify possible prognostic and therapeutic targets in these neoplasms. Fifteen canine STS samples were evaluated and were submitted to immunohistochemistry for acetylated histones 3 (H3) and 4 (H4) and deacetylating enzymes (HDAC1, HDAC2 and HDAC6). Intense immunolabelling of H4 was seen in comparison with H3. A strong positive correlation was observed between the H3 intensity score and the number of mitotic figures (P = 0.004, r = 0.7). Intense immunolabelling of HDAC1 was found in comparison to the expression of HDAC2 and HDAC6 in the evaluated STSs. This finding suggests that HDAC1 may be a potential target for HDAC inhibitors in STSs in dogs.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-01
2023-03-01T19:51:23Z
2023-03-01T19:51:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jcpa.2022.03.001
Journal of Comparative Pathology, v. 193, p. 51-58.
1532-3129
0021-9975
http://hdl.handle.net/11449/239877
10.1016/j.jcpa.2022.03.001
2-s2.0-85128184230
url http://dx.doi.org/10.1016/j.jcpa.2022.03.001
http://hdl.handle.net/11449/239877
identifier_str_mv Journal of Comparative Pathology, v. 193, p. 51-58.
1532-3129
0021-9975
10.1016/j.jcpa.2022.03.001
2-s2.0-85128184230
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Comparative Pathology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 51-58
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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