Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jcpa.2022.03.001 http://hdl.handle.net/11449/239877 |
Resumo: | The objective of this study was to investigate the immunolabelling of acetylated histones and histone desacetylase (HDAC) enzymes in canine soft tissue sarcomas (STSs) and to correlate them with histological and clinical features in order to identify possible prognostic and therapeutic targets in these neoplasms. Fifteen canine STS samples were evaluated and were submitted to immunohistochemistry for acetylated histones 3 (H3) and 4 (H4) and deacetylating enzymes (HDAC1, HDAC2 and HDAC6). Intense immunolabelling of H4 was seen in comparison with H3. A strong positive correlation was observed between the H3 intensity score and the number of mitotic figures (P = 0.004, r = 0.7). Intense immunolabelling of HDAC1 was found in comparison to the expression of HDAC2 and HDAC6 in the evaluated STSs. This finding suggests that HDAC1 may be a potential target for HDAC inhibitors in STSs in dogs. |
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Repositório Institucional da UNESP |
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Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomascanineepigenetichistonesmesenchymal neoplasiaThe objective of this study was to investigate the immunolabelling of acetylated histones and histone desacetylase (HDAC) enzymes in canine soft tissue sarcomas (STSs) and to correlate them with histological and clinical features in order to identify possible prognostic and therapeutic targets in these neoplasms. Fifteen canine STS samples were evaluated and were submitted to immunohistochemistry for acetylated histones 3 (H3) and 4 (H4) and deacetylating enzymes (HDAC1, HDAC2 and HDAC6). Intense immunolabelling of H4 was seen in comparison with H3. A strong positive correlation was observed between the H3 intensity score and the number of mitotic figures (P = 0.004, r = 0.7). Intense immunolabelling of HDAC1 was found in comparison to the expression of HDAC2 and HDAC6 in the evaluated STSs. This finding suggests that HDAC1 may be a potential target for HDAC inhibitors in STSs in dogs.Department of Veterinary Medicine and Surgery Veterinay School Universidade Federal de Minas Gerais, MGDepartment of Veterinary Medicine Universidade Vila Velha, ESAutonomous Veterinarian Life – Oncologia VeterináriaDepartment of Clinical and Veterinary Surgery College of Agricultural and Veterinary Sciences Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, SPAutonomous Veterinarian, ESVeterinary Hospital Universidade Federal do Vale do São Francisco HU-Univasf, PEVETPAT Pathology and Molecular Biology Laboratory, SPDepartment of Clinical and Veterinary Surgery College of Agricultural and Veterinary Sciences Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, SPUniversidade Federal de Minas Gerais (UFMG)Universidade Vila VelhaLife – Oncologia VeterináriaUniversidade Estadual Paulista (UNESP)Autonomous VeterinarianHU-UnivasfVETPAT Pathology and Molecular Biology LaboratorySena, Bruna V.Horta, Rodrigo S.Senhorello, Igor L.S.Matiz, Oscar R.S.Canavari, Isabela C. [UNESP]Souza, Larissa M.Ferreira, Marília G.P.A.Sueiro, Felipe A.R.Costa, Mirela T. [UNESP]Nardi, Andrigo B. de[UNESP]2023-03-01T19:51:23Z2023-03-01T19:51:23Z2022-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article51-58http://dx.doi.org/10.1016/j.jcpa.2022.03.001Journal of Comparative Pathology, v. 193, p. 51-58.1532-31290021-9975http://hdl.handle.net/11449/23987710.1016/j.jcpa.2022.03.0012-s2.0-85128184230Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Comparative Pathologyinfo:eu-repo/semantics/openAccess2023-03-01T19:51:23Zoai:repositorio.unesp.br:11449/239877Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:06:05.514906Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas |
title |
Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas |
spellingShingle |
Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas Sena, Bruna V. canine epigenetic histones mesenchymal neoplasia |
title_short |
Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas |
title_full |
Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas |
title_fullStr |
Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas |
title_full_unstemmed |
Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas |
title_sort |
Immunolabelling of Acetylated Histones 3 and 4 and Histone Deacetylases 1, 2 and 6 in Canine Soft Tissue Sarcomas |
author |
Sena, Bruna V. |
author_facet |
Sena, Bruna V. Horta, Rodrigo S. Senhorello, Igor L.S. Matiz, Oscar R.S. Canavari, Isabela C. [UNESP] Souza, Larissa M. Ferreira, Marília G.P.A. Sueiro, Felipe A.R. Costa, Mirela T. [UNESP] Nardi, Andrigo B. de[UNESP] |
author_role |
author |
author2 |
Horta, Rodrigo S. Senhorello, Igor L.S. Matiz, Oscar R.S. Canavari, Isabela C. [UNESP] Souza, Larissa M. Ferreira, Marília G.P.A. Sueiro, Felipe A.R. Costa, Mirela T. [UNESP] Nardi, Andrigo B. de[UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG) Universidade Vila Velha Life – Oncologia Veterinária Universidade Estadual Paulista (UNESP) Autonomous Veterinarian HU-Univasf VETPAT Pathology and Molecular Biology Laboratory |
dc.contributor.author.fl_str_mv |
Sena, Bruna V. Horta, Rodrigo S. Senhorello, Igor L.S. Matiz, Oscar R.S. Canavari, Isabela C. [UNESP] Souza, Larissa M. Ferreira, Marília G.P.A. Sueiro, Felipe A.R. Costa, Mirela T. [UNESP] Nardi, Andrigo B. de[UNESP] |
dc.subject.por.fl_str_mv |
canine epigenetic histones mesenchymal neoplasia |
topic |
canine epigenetic histones mesenchymal neoplasia |
description |
The objective of this study was to investigate the immunolabelling of acetylated histones and histone desacetylase (HDAC) enzymes in canine soft tissue sarcomas (STSs) and to correlate them with histological and clinical features in order to identify possible prognostic and therapeutic targets in these neoplasms. Fifteen canine STS samples were evaluated and were submitted to immunohistochemistry for acetylated histones 3 (H3) and 4 (H4) and deacetylating enzymes (HDAC1, HDAC2 and HDAC6). Intense immunolabelling of H4 was seen in comparison with H3. A strong positive correlation was observed between the H3 intensity score and the number of mitotic figures (P = 0.004, r = 0.7). Intense immunolabelling of HDAC1 was found in comparison to the expression of HDAC2 and HDAC6 in the evaluated STSs. This finding suggests that HDAC1 may be a potential target for HDAC inhibitors in STSs in dogs. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01 2023-03-01T19:51:23Z 2023-03-01T19:51:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jcpa.2022.03.001 Journal of Comparative Pathology, v. 193, p. 51-58. 1532-3129 0021-9975 http://hdl.handle.net/11449/239877 10.1016/j.jcpa.2022.03.001 2-s2.0-85128184230 |
url |
http://dx.doi.org/10.1016/j.jcpa.2022.03.001 http://hdl.handle.net/11449/239877 |
identifier_str_mv |
Journal of Comparative Pathology, v. 193, p. 51-58. 1532-3129 0021-9975 10.1016/j.jcpa.2022.03.001 2-s2.0-85128184230 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Comparative Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
51-58 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129283882745856 |