Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.

Detalhes bibliográficos
Autor(a) principal: Machado, R. Z. [UNESP]
Data de Publicação: 1994
Outros Autores: Valadão, C. A. [UNESP], Melo, W. R. [UNESP], Alessi, A. C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/223984
Resumo: 1.We describe the isolation of viable merozoites from erythrocytes infected with Babesia bovis or Babesia bigemina organisms by ammonium chloride lysis. 2. Parasite morphology was examined by both light and transmission electron microscopy. Erythrocyte-free parasites maintain their viability and infectivity, retain their antigenicity and are suitable for use in the indirect fluorescent antibody assay.
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spelling Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.1.We describe the isolation of viable merozoites from erythrocytes infected with Babesia bovis or Babesia bigemina organisms by ammonium chloride lysis. 2. Parasite morphology was examined by both light and transmission electron microscopy. Erythrocyte-free parasites maintain their viability and infectivity, retain their antigenicity and are suitable for use in the indirect fluorescent antibody assay.Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias UNESP SPDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias UNESP SPUniversidade Estadual Paulista (UNESP)Machado, R. Z. [UNESP]Valadão, C. A. [UNESP]Melo, W. R. [UNESP]Alessi, A. C. [UNESP]2022-04-28T19:54:02Z2022-04-28T19:54:02Z1994-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2591-2598Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], v. 27, n. 11, p. 2591-2598, 1994.0100-879Xhttp://hdl.handle.net/11449/2239842-s2.0-0028543544Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]info:eu-repo/semantics/openAccess2022-04-28T19:54:02Zoai:repositorio.unesp.br:11449/223984Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:54:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.
title Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.
spellingShingle Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.
Machado, R. Z. [UNESP]
title_short Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.
title_full Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.
title_fullStr Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.
title_full_unstemmed Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.
title_sort Isolation of Babesia bigemina and babesia bovis merozoites by ammonium chloride lysis of infected erythrocytes.
author Machado, R. Z. [UNESP]
author_facet Machado, R. Z. [UNESP]
Valadão, C. A. [UNESP]
Melo, W. R. [UNESP]
Alessi, A. C. [UNESP]
author_role author
author2 Valadão, C. A. [UNESP]
Melo, W. R. [UNESP]
Alessi, A. C. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Machado, R. Z. [UNESP]
Valadão, C. A. [UNESP]
Melo, W. R. [UNESP]
Alessi, A. C. [UNESP]
description 1.We describe the isolation of viable merozoites from erythrocytes infected with Babesia bovis or Babesia bigemina organisms by ammonium chloride lysis. 2. Parasite morphology was examined by both light and transmission electron microscopy. Erythrocyte-free parasites maintain their viability and infectivity, retain their antigenicity and are suitable for use in the indirect fluorescent antibody assay.
publishDate 1994
dc.date.none.fl_str_mv 1994-01-01
2022-04-28T19:54:02Z
2022-04-28T19:54:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], v. 27, n. 11, p. 2591-2598, 1994.
0100-879X
http://hdl.handle.net/11449/223984
2-s2.0-0028543544
identifier_str_mv Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.], v. 27, n. 11, p. 2591-2598, 1994.
0100-879X
2-s2.0-0028543544
url http://hdl.handle.net/11449/223984
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2591-2598
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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