Gene mutations in esophageal mucosa of chagas disease patients

• Autor(a) principal: Manoel-Caetano, Fernanda DA Silva [UNESP]
• Data de Publicação: 2009
• Outros Autores: Silveira, Aparecida F. P. [UNESP], Silva, Ana Elizabete [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/225457
• topic: Cdkn2a gene; Chagas disease; Dna sequencing; Fhitgene; Megaesophagus, gene mutation; Single nucleotide polymorphism (snp); Tp53 gene

Background: Chagas disease patients with longstanding megaesophagus have a significantly increased risk for esophageal carcinoma. Materials and Methods: PCR- SSCP analysis and DNA sequencing of esophageal mucosa from Chagas disease patients (with or without megaesophagus) in the exons of the TPS3, CDKN2A and FHIT genes were performed. Results: SSCP analysis showed a mobility shift in 2/20 patients with grade TV megaesophagus (CDKN2A exon 1 and FHIT exon 7) and in 1/10 patients without megaesophagus (TP53 exons 5 and 8). However, DNA sequencing indicated a silent mutation in exon 7 of the FHIT gene (GCT → GCC) in codon 88 in only one case of megaesophagus. Conclusion: Gene mutations are rare events in the exons investigated in esophageal mucosa of Chagas disease patients and further investigations are important to clarify the possible involvement of this silent mutation in exon 7 (FHIT gene) in the advanced grades of megaesophagus and esophageal carcinogenesis.