Counting of conjuntival cells by impression cytology in bovine and equine

• Autor(a) principal: Gonçalves, Roberto Calderon [UNESP]
• Data de Publicação: 2012
• Outros Autores: Pereira, Eric de Castro [UNESP], Flórez, Luis Mauricio Montoya, Pedraza, Francisco Javier, Chiacchio, Simone Biagio [UNESP], Amorim, Rogério Martins [UNESP], Borges, Alexandre Secorun [UNESP], Rocha, Noeme Sousa [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://revistas.ulusofona.pt/index.php/rlcmv/article/view/3019; http://hdl.handle.net/11449/140486
• topic: Cytology; Ophthalmology; Cattle; Horses; Citologia; Oftalmologia; Bovino; Equino

Impression cytology is a useful, non-invasive method of investigating the ocular surface. This method consists of applying a piece of filter paper to the conjunctiva. The samples obtained can comprise up to three cell layers, which are applied to a histological slide. This study aimed to evaluate the quality and quantity of cells in the bulbar conjunctivas of clinically healthy cattle and horses to facilitate the diagnosis of external eye diseases. To this end, samples of external ocular conjunctiva cells were collected from 15 adult cattle and 15 adult horses using the impression cytology method. For cytology, the samples were stained with Giemsa and Shorr. Two slides were prepared for each animal, one from the left eye and one from the right eye, and 800 cells were counted in total. For the statistical analysis, ANOVA was used with p<0.05 considered to be statistically significant. The analyses allowed for the comparison of the numbers of goblet cells, lymphocytes, and erythrocytes between the species and among the sampled animals. Impression cytology of the ocular surface was effective, and the technique was well accepted by cattle and horses.