Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucrose
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/231306 |
Resumo: | Food restriction reduces body weight and influence bone mass and also is correlated with bone mineral density (BMD). Mechanisms have been proposed for the loss of BMD after body weight reduction, including reduced energy intake. Growing 8 wk-old Wistar male rats were randomly divided into Control and Calorie restriction associated with sucrose 30% (CRS). These animals were subjected to intermittent food restriction during 8 weeks and had free access to tap water and sucrose30% in distilled water. The rats were euthanized at the end of week 8, blood collected from abdominal aorta artery, femurs cleaned of adherent soft tissues, scanned using dual energy X-ray absorptiometry, structural and material properties determined by three-point bending testing in the mid-diaphyseal region, bone surface tested in a microhardness tester and microstructure was assessed in a microcomputer tomography. In CRS animals body weight decreased significantly relative to the Control animals. There was a clear option for high-sucrose beverage in CRS animals. No difference was observed in biochemical, densitometric and biomechanical analyzes. Results from micro CT showed only significant difference in connectivity of trabecular bone. It has been suggested that rats submitted to food restriction consumed sugar not because of its inherent palatability, but in order to alter their macronutrient balance and animals need to meet energy demands in high-sucrose. |
id |
UNSP_8835c3220d3ddbb01c51e7ff68fdcd5d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/231306 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucroseBone mineral densityFemoral biomechanicsFemoral microtomographyFood restrictionFood restriction reduces body weight and influence bone mass and also is correlated with bone mineral density (BMD). Mechanisms have been proposed for the loss of BMD after body weight reduction, including reduced energy intake. Growing 8 wk-old Wistar male rats were randomly divided into Control and Calorie restriction associated with sucrose 30% (CRS). These animals were subjected to intermittent food restriction during 8 weeks and had free access to tap water and sucrose30% in distilled water. The rats were euthanized at the end of week 8, blood collected from abdominal aorta artery, femurs cleaned of adherent soft tissues, scanned using dual energy X-ray absorptiometry, structural and material properties determined by three-point bending testing in the mid-diaphyseal region, bone surface tested in a microhardness tester and microstructure was assessed in a microcomputer tomography. In CRS animals body weight decreased significantly relative to the Control animals. There was a clear option for high-sucrose beverage in CRS animals. No difference was observed in biochemical, densitometric and biomechanical analyzes. Results from micro CT showed only significant difference in connectivity of trabecular bone. It has been suggested that rats submitted to food restriction consumed sugar not because of its inherent palatability, but in order to alter their macronutrient balance and animals need to meet energy demands in high-sucrose.Department of Pathology Araçatuba Dental School, CEP 16015-050, Araçatuba, SPDepartment of Physiology Sciences Federal University of São Carlos - UFSCar, São Carlos, SPDepartment of Basic Sciences Araçatuba Dental School, CEP 16015-050, Araçatuba, SPDepartment of Support, Production and Animal Health Medicine Veterinarian School at Araçatuba, CEP 16015-050, Araçatuba, SPAraçatuba Dental SchoolUniversidade Federal de São Carlos (UFSCar)Medicine Veterinarian School at AraçatubaCarvalho, A. A.F.Biffe, B. G.Nakamune, Ana Cláudia de Melo Stevanato [UNESP]Louzada, M. J.Q.2022-04-29T08:44:44Z2022-04-29T08:44:44Z2013-10-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article176-181Journal of Morphological Sciences, v. 30, n. 3, p. 176-181, 2013.2177-0298http://hdl.handle.net/11449/2313062-s2.0-84885721070Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Morphological Sciencesinfo:eu-repo/semantics/openAccess2024-09-04T19:15:25Zoai:repositorio.unesp.br:11449/231306Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucrose |
title |
Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucrose |
spellingShingle |
Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucrose Carvalho, A. A.F. Bone mineral density Femoral biomechanics Femoral microtomography Food restriction |
title_short |
Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucrose |
title_full |
Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucrose |
title_fullStr |
Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucrose |
title_full_unstemmed |
Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucrose |
title_sort |
Femoral biomechanic and microtomography from male rats submitted to dietary restriction supplemented with sucrose |
author |
Carvalho, A. A.F. |
author_facet |
Carvalho, A. A.F. Biffe, B. G. Nakamune, Ana Cláudia de Melo Stevanato [UNESP] Louzada, M. J.Q. |
author_role |
author |
author2 |
Biffe, B. G. Nakamune, Ana Cláudia de Melo Stevanato [UNESP] Louzada, M. J.Q. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Araçatuba Dental School Universidade Federal de São Carlos (UFSCar) Medicine Veterinarian School at Araçatuba |
dc.contributor.author.fl_str_mv |
Carvalho, A. A.F. Biffe, B. G. Nakamune, Ana Cláudia de Melo Stevanato [UNESP] Louzada, M. J.Q. |
dc.subject.por.fl_str_mv |
Bone mineral density Femoral biomechanics Femoral microtomography Food restriction |
topic |
Bone mineral density Femoral biomechanics Femoral microtomography Food restriction |
description |
Food restriction reduces body weight and influence bone mass and also is correlated with bone mineral density (BMD). Mechanisms have been proposed for the loss of BMD after body weight reduction, including reduced energy intake. Growing 8 wk-old Wistar male rats were randomly divided into Control and Calorie restriction associated with sucrose 30% (CRS). These animals were subjected to intermittent food restriction during 8 weeks and had free access to tap water and sucrose30% in distilled water. The rats were euthanized at the end of week 8, blood collected from abdominal aorta artery, femurs cleaned of adherent soft tissues, scanned using dual energy X-ray absorptiometry, structural and material properties determined by three-point bending testing in the mid-diaphyseal region, bone surface tested in a microhardness tester and microstructure was assessed in a microcomputer tomography. In CRS animals body weight decreased significantly relative to the Control animals. There was a clear option for high-sucrose beverage in CRS animals. No difference was observed in biochemical, densitometric and biomechanical analyzes. Results from micro CT showed only significant difference in connectivity of trabecular bone. It has been suggested that rats submitted to food restriction consumed sugar not because of its inherent palatability, but in order to alter their macronutrient balance and animals need to meet energy demands in high-sucrose. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-23 2022-04-29T08:44:44Z 2022-04-29T08:44:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Morphological Sciences, v. 30, n. 3, p. 176-181, 2013. 2177-0298 http://hdl.handle.net/11449/231306 2-s2.0-84885721070 |
identifier_str_mv |
Journal of Morphological Sciences, v. 30, n. 3, p. 176-181, 2013. 2177-0298 2-s2.0-84885721070 |
url |
http://hdl.handle.net/11449/231306 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Morphological Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
176-181 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021378902982656 |