Investigation of the state of virus carrier of newcastle disease in budgerigars (melopsittacus undulatus)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3923/ijps.2013.135.137 http://hdl.handle.net/11449/75419 |
Resumo: | This study aimed to evaluate the importance of vaccination against Newcastle Disease (ND) in budgerigars (Melopsittacus undulatus) and to investigate the state of carrier of the virus (NDV) in this species. There were used 72 budgerigars distributed into four different experimental groups, vaccinated or not against ND: GI (Ulster 2C strain), GII (B1 strain), GIII (LaSota strain) and GIV (not vaccinated-control). At 11 months of age, all groups of birds were challenged with a pathogenic virus (NDV) suspension, EID50 = 108.15/0.1 mL. A group of Specific-Pathogen-Free (SPF) chickens were used as control of the virus. Cloacal swabs and blood samples were collected after 13 and 19 days post-challenge, respectively, for genome viral excretion by Reverse Transcription Polymerase Chain Reaction (RT-PCR) and antibody's level by the inhibition of hemagglutination test (HI). Budgerigars of all groups (including the control group-not vaccinated) did not demonstrate any signs of Newcastle Disease. They were refractory to the clinical disease with the NDV. Budgerigars from the control group (not vaccinated), showed antibody titers from HI test 13 and 19 days after challenge. Therefore, it was demonstrated the state of carrier of NDV in this species. The birds, from the vaccinated groups did not demonstrate genome viral excretion by RT-PCR and antibody titles were not detected by HI tests, respectively. It was also demonstrated the importance of the vaccination in the suppression of the state of carrier of NDV in budgerigars. © Asian Network for Scientific Information, 2013. |
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Repositório Institucional da UNESP |
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Investigation of the state of virus carrier of newcastle disease in budgerigars (melopsittacus undulatus)BudgerigarsMelopsittacus undulatusNDV carrierNewcastle diseaseVaccinationThis study aimed to evaluate the importance of vaccination against Newcastle Disease (ND) in budgerigars (Melopsittacus undulatus) and to investigate the state of carrier of the virus (NDV) in this species. There were used 72 budgerigars distributed into four different experimental groups, vaccinated or not against ND: GI (Ulster 2C strain), GII (B1 strain), GIII (LaSota strain) and GIV (not vaccinated-control). At 11 months of age, all groups of birds were challenged with a pathogenic virus (NDV) suspension, EID50 = 108.15/0.1 mL. A group of Specific-Pathogen-Free (SPF) chickens were used as control of the virus. Cloacal swabs and blood samples were collected after 13 and 19 days post-challenge, respectively, for genome viral excretion by Reverse Transcription Polymerase Chain Reaction (RT-PCR) and antibody's level by the inhibition of hemagglutination test (HI). Budgerigars of all groups (including the control group-not vaccinated) did not demonstrate any signs of Newcastle Disease. They were refractory to the clinical disease with the NDV. Budgerigars from the control group (not vaccinated), showed antibody titers from HI test 13 and 19 days after challenge. Therefore, it was demonstrated the state of carrier of NDV in this species. The birds, from the vaccinated groups did not demonstrate genome viral excretion by RT-PCR and antibody titles were not detected by HI tests, respectively. It was also demonstrated the importance of the vaccination in the suppression of the state of carrier of NDV in budgerigars. © Asian Network for Scientific Information, 2013.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Alunos Programa de Pós-graduação em Medicina Veterinária da FCAV Unesp, JaboticabalBolsistas FAPESPDepartamento de Patologia Veterinária FCAV Unesp, JaboticabalCNPqDepartamento de Clínica Veterinária FMVZ Unesp, BotucatuUniversidade Estadual do Centro-Oeste UNICENTRO, ParanáAlunos Programa de Pós-graduação em Medicina Veterinária da FCAV Unesp, JaboticabalDepartamento de Patologia Veterinária FCAV Unesp, JaboticabalDepartamento de Clínica Veterinária FMVZ Unesp, BotucatuUniversidade Estadual Paulista (Unesp)Universidade Estadual do Centro Oeste (UNICENTRO)Denadai, Janine [UNESP]Paulillo, Antônio Carlos [UNESP]Schmidt, Elizabeth Moreira dos Santos [UNESP]Martins, Gislaine Regina Vieira [UNESP]Torres Carrasco, Adriano de Oliveira2014-05-27T11:29:30Z2014-05-27T11:29:30Z2013-05-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article135-137http://dx.doi.org/10.3923/ijps.2013.135.137International Journal of Poultry Science, v. 12, n. 3, p. 135-137, 2013.1682-8356http://hdl.handle.net/11449/7541910.3923/ijps.2013.135.1372-s2.0-848777811647255270378750464Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Poultry Science0,238info:eu-repo/semantics/openAccess2024-06-07T13:01:55Zoai:repositorio.unesp.br:11449/75419Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:47:12.708925Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigation of the state of virus carrier of newcastle disease in budgerigars (melopsittacus undulatus) |
title |
Investigation of the state of virus carrier of newcastle disease in budgerigars (melopsittacus undulatus) |
spellingShingle |
Investigation of the state of virus carrier of newcastle disease in budgerigars (melopsittacus undulatus) Denadai, Janine [UNESP] Budgerigars Melopsittacus undulatus NDV carrier Newcastle disease Vaccination |
title_short |
Investigation of the state of virus carrier of newcastle disease in budgerigars (melopsittacus undulatus) |
title_full |
Investigation of the state of virus carrier of newcastle disease in budgerigars (melopsittacus undulatus) |
title_fullStr |
Investigation of the state of virus carrier of newcastle disease in budgerigars (melopsittacus undulatus) |
title_full_unstemmed |
Investigation of the state of virus carrier of newcastle disease in budgerigars (melopsittacus undulatus) |
title_sort |
Investigation of the state of virus carrier of newcastle disease in budgerigars (melopsittacus undulatus) |
author |
Denadai, Janine [UNESP] |
author_facet |
Denadai, Janine [UNESP] Paulillo, Antônio Carlos [UNESP] Schmidt, Elizabeth Moreira dos Santos [UNESP] Martins, Gislaine Regina Vieira [UNESP] Torres Carrasco, Adriano de Oliveira |
author_role |
author |
author2 |
Paulillo, Antônio Carlos [UNESP] Schmidt, Elizabeth Moreira dos Santos [UNESP] Martins, Gislaine Regina Vieira [UNESP] Torres Carrasco, Adriano de Oliveira |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual do Centro Oeste (UNICENTRO) |
dc.contributor.author.fl_str_mv |
Denadai, Janine [UNESP] Paulillo, Antônio Carlos [UNESP] Schmidt, Elizabeth Moreira dos Santos [UNESP] Martins, Gislaine Regina Vieira [UNESP] Torres Carrasco, Adriano de Oliveira |
dc.subject.por.fl_str_mv |
Budgerigars Melopsittacus undulatus NDV carrier Newcastle disease Vaccination |
topic |
Budgerigars Melopsittacus undulatus NDV carrier Newcastle disease Vaccination |
description |
This study aimed to evaluate the importance of vaccination against Newcastle Disease (ND) in budgerigars (Melopsittacus undulatus) and to investigate the state of carrier of the virus (NDV) in this species. There were used 72 budgerigars distributed into four different experimental groups, vaccinated or not against ND: GI (Ulster 2C strain), GII (B1 strain), GIII (LaSota strain) and GIV (not vaccinated-control). At 11 months of age, all groups of birds were challenged with a pathogenic virus (NDV) suspension, EID50 = 108.15/0.1 mL. A group of Specific-Pathogen-Free (SPF) chickens were used as control of the virus. Cloacal swabs and blood samples were collected after 13 and 19 days post-challenge, respectively, for genome viral excretion by Reverse Transcription Polymerase Chain Reaction (RT-PCR) and antibody's level by the inhibition of hemagglutination test (HI). Budgerigars of all groups (including the control group-not vaccinated) did not demonstrate any signs of Newcastle Disease. They were refractory to the clinical disease with the NDV. Budgerigars from the control group (not vaccinated), showed antibody titers from HI test 13 and 19 days after challenge. Therefore, it was demonstrated the state of carrier of NDV in this species. The birds, from the vaccinated groups did not demonstrate genome viral excretion by RT-PCR and antibody titles were not detected by HI tests, respectively. It was also demonstrated the importance of the vaccination in the suppression of the state of carrier of NDV in budgerigars. © Asian Network for Scientific Information, 2013. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-20 2014-05-27T11:29:30Z 2014-05-27T11:29:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3923/ijps.2013.135.137 International Journal of Poultry Science, v. 12, n. 3, p. 135-137, 2013. 1682-8356 http://hdl.handle.net/11449/75419 10.3923/ijps.2013.135.137 2-s2.0-84877781164 7255270378750464 |
url |
http://dx.doi.org/10.3923/ijps.2013.135.137 http://hdl.handle.net/11449/75419 |
identifier_str_mv |
International Journal of Poultry Science, v. 12, n. 3, p. 135-137, 2013. 1682-8356 10.3923/ijps.2013.135.137 2-s2.0-84877781164 7255270378750464 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Poultry Science 0,238 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
135-137 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128700770680832 |