Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12941-016-0125-5 http://hdl.handle.net/11449/172525 |
Resumo: | Background: Staphylococcus aureus is characterized by its pathogenicity and high prevalence, causing disease in both healthy and immunocompromised individuals due to its easy dissemination. This fact is aggravated by the widespread dissemination of S. aureus carrying toxigenic genes. The objective of this study was to determine the toxigenic profile of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in patients with purulent skin and/or soft tissue infections seen at the Dermatology Department of the University Hospital of the Botucatu Medical School, asymptomatic adults older than 60years living in nursing homes, and prison inmates of the Avaré Detention Center. Methods: PCR was used for the detection of the mecA gene, enterotoxin genes (sea, seb, and sec), exfoliative toxins A and B (eta and etb), toxic shock syndrome toxin 1 (tst), panton-valentine leukocidin (lukS-PV and lukF-PV), and alpha- and delta-hemolysins or cytotoxins (hla and hld). Results: The results showed a significant prevalence of toxigenic genes among S. aureus isolates from asymptomatic individuals, with the observation of a higher prevalence of cytotoxin genes. However, the panton-valentine leukocidin gene was only detected in MSSA isolated from patients with skin infections and the tst gene was exclusively found in MSSA isolated from prison inmates. Conclusions: The present study demonstrated a significant prevalence of toxigenic genes in MSSA and MRSA strains isolated from asymptomatic S. aureus carriers. There was a higher prevalence of cytotoxin genes. |
id |
UNSP_890a9e5a2256b46a3c0b1c602d46a2f0 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/172525 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groupsCytotoxinsMSSAStaphylococcus aureusToxinsBackground: Staphylococcus aureus is characterized by its pathogenicity and high prevalence, causing disease in both healthy and immunocompromised individuals due to its easy dissemination. This fact is aggravated by the widespread dissemination of S. aureus carrying toxigenic genes. The objective of this study was to determine the toxigenic profile of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in patients with purulent skin and/or soft tissue infections seen at the Dermatology Department of the University Hospital of the Botucatu Medical School, asymptomatic adults older than 60years living in nursing homes, and prison inmates of the Avaré Detention Center. Methods: PCR was used for the detection of the mecA gene, enterotoxin genes (sea, seb, and sec), exfoliative toxins A and B (eta and etb), toxic shock syndrome toxin 1 (tst), panton-valentine leukocidin (lukS-PV and lukF-PV), and alpha- and delta-hemolysins or cytotoxins (hla and hld). Results: The results showed a significant prevalence of toxigenic genes among S. aureus isolates from asymptomatic individuals, with the observation of a higher prevalence of cytotoxin genes. However, the panton-valentine leukocidin gene was only detected in MSSA isolated from patients with skin infections and the tst gene was exclusively found in MSSA isolated from prison inmates. Conclusions: The present study demonstrated a significant prevalence of toxigenic genes in MSSA and MRSA strains isolated from asymptomatic S. aureus carriers. There was a higher prevalence of cytotoxin genes.UNESP-Univ Estadual Paulista Department of Microbiology and Immunology Botucatu Biosciences InstituteUniversity Hospital UNESP-Univ Estadual Paulista Department of Tropical Diseases Botucatu School of MedicineUniversity Hospital UNESP-Univ Estadual Paulista Department of Dermatology and Radiology Botucatu School of MedicineUNESP-Univ Estadual Paulista Department of Microbiology and Immunology Botucatu Biosciences InstituteUniversity Hospital UNESP-Univ Estadual Paulista Department of Tropical Diseases Botucatu School of MedicineUniversity Hospital UNESP-Univ Estadual Paulista Department of Dermatology and Radiology Botucatu School of MedicineUniversidade Estadual Paulista (Unesp)Souza, Camila Sena Martins [UNESP]Fortaleza, Carlos Magno Castelo Branco [UNESP]Witzel, Claudia Lima [UNESP]Silveira, Mônica [UNESP]Bonesso, Mariana Fávero [UNESP]Marques, Silvio Alencar [UNESP]Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP]2018-12-11T17:00:48Z2018-12-11T17:00:48Z2016-02-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12941-016-0125-5Annals of Clinical Microbiology and Antimicrobials, v. 15, n. 1, 2016.1476-0711http://hdl.handle.net/11449/17252510.1186/s12941-016-0125-52-s2.0-849579578892-s2.0-84957957889.pdf0115647772315973Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnnals of Clinical Microbiology and Antimicrobials1,104info:eu-repo/semantics/openAccess2024-01-18T06:29:57Zoai:repositorio.unesp.br:11449/172525Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-18T06:29:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups |
title |
Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups |
spellingShingle |
Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups Souza, Camila Sena Martins [UNESP] Cytotoxins MSSA Staphylococcus aureus Toxins |
title_short |
Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups |
title_full |
Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups |
title_fullStr |
Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups |
title_full_unstemmed |
Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups |
title_sort |
Toxigenic profile of methicillin-sensitive and resistant Staphylococcus aureus isolated from special groups |
author |
Souza, Camila Sena Martins [UNESP] |
author_facet |
Souza, Camila Sena Martins [UNESP] Fortaleza, Carlos Magno Castelo Branco [UNESP] Witzel, Claudia Lima [UNESP] Silveira, Mônica [UNESP] Bonesso, Mariana Fávero [UNESP] Marques, Silvio Alencar [UNESP] Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP] |
author_role |
author |
author2 |
Fortaleza, Carlos Magno Castelo Branco [UNESP] Witzel, Claudia Lima [UNESP] Silveira, Mônica [UNESP] Bonesso, Mariana Fávero [UNESP] Marques, Silvio Alencar [UNESP] Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Souza, Camila Sena Martins [UNESP] Fortaleza, Carlos Magno Castelo Branco [UNESP] Witzel, Claudia Lima [UNESP] Silveira, Mônica [UNESP] Bonesso, Mariana Fávero [UNESP] Marques, Silvio Alencar [UNESP] Cunha, Maria de Lourdes Ribeiro de Souza da [UNESP] |
dc.subject.por.fl_str_mv |
Cytotoxins MSSA Staphylococcus aureus Toxins |
topic |
Cytotoxins MSSA Staphylococcus aureus Toxins |
description |
Background: Staphylococcus aureus is characterized by its pathogenicity and high prevalence, causing disease in both healthy and immunocompromised individuals due to its easy dissemination. This fact is aggravated by the widespread dissemination of S. aureus carrying toxigenic genes. The objective of this study was to determine the toxigenic profile of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in patients with purulent skin and/or soft tissue infections seen at the Dermatology Department of the University Hospital of the Botucatu Medical School, asymptomatic adults older than 60years living in nursing homes, and prison inmates of the Avaré Detention Center. Methods: PCR was used for the detection of the mecA gene, enterotoxin genes (sea, seb, and sec), exfoliative toxins A and B (eta and etb), toxic shock syndrome toxin 1 (tst), panton-valentine leukocidin (lukS-PV and lukF-PV), and alpha- and delta-hemolysins or cytotoxins (hla and hld). Results: The results showed a significant prevalence of toxigenic genes among S. aureus isolates from asymptomatic individuals, with the observation of a higher prevalence of cytotoxin genes. However, the panton-valentine leukocidin gene was only detected in MSSA isolated from patients with skin infections and the tst gene was exclusively found in MSSA isolated from prison inmates. Conclusions: The present study demonstrated a significant prevalence of toxigenic genes in MSSA and MRSA strains isolated from asymptomatic S. aureus carriers. There was a higher prevalence of cytotoxin genes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-16 2018-12-11T17:00:48Z 2018-12-11T17:00:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12941-016-0125-5 Annals of Clinical Microbiology and Antimicrobials, v. 15, n. 1, 2016. 1476-0711 http://hdl.handle.net/11449/172525 10.1186/s12941-016-0125-5 2-s2.0-84957957889 2-s2.0-84957957889.pdf 0115647772315973 |
url |
http://dx.doi.org/10.1186/s12941-016-0125-5 http://hdl.handle.net/11449/172525 |
identifier_str_mv |
Annals of Clinical Microbiology and Antimicrobials, v. 15, n. 1, 2016. 1476-0711 10.1186/s12941-016-0125-5 2-s2.0-84957957889 2-s2.0-84957957889.pdf 0115647772315973 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Annals of Clinical Microbiology and Antimicrobials 1,104 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965657865912320 |