Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3892/ol.2020.12019 http://hdl.handle.net/11449/209666 |
Resumo: | The epithelial-to-mesenchymal transition (EMT) is a phenomenon during which cancer epithelial cells undergo changes in plasticity and lose cell-cell adhesion with consequent remodeling of the extracellular matrix and development of mesenchymal characteristics. Long non-coding RNAs (lncRNAs) have been described as EMT modulation markers, becoming a promising target in the development of new therapies for cancer. The present study aimed to investigate the role of everolimus at 100 nM as inductor of the EMT phenomenon in cell lines derived from human breast (BT-549), colorectal (RKO-AS45-1) and ovary (TOV-21G) cancer. The integrity of cellular junctions was monitored using an in vitro model of epithelial resistance. The results demonstrated that the EMT genes ZEB1, TWIST1 and TGFB1 were differentially expressed in cells treated with everolimus compared with in untreated cells. lncRNA HOTAIR was upregulated post-treatment only in BT-549 cells compared with in untreated cells. After treatment with everolimus, the intensity of fluorescence of P-cadherin decreased, and that of fibronectin increased in RKO-AS45-1 and TOV-21G cells compared with control cells. The transepithelial electrical resistance at the RKO-AS45-1 monolayer treated with everolimus started to decrease at 48 h. The changes in the gene expression and epithelial resistance may confirm the role of everolimus in EMT. |
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Repositório Institucional da UNESP |
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spelling |
Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatmentEMTcancereverolimusHOTAIRbiomarkerThe epithelial-to-mesenchymal transition (EMT) is a phenomenon during which cancer epithelial cells undergo changes in plasticity and lose cell-cell adhesion with consequent remodeling of the extracellular matrix and development of mesenchymal characteristics. Long non-coding RNAs (lncRNAs) have been described as EMT modulation markers, becoming a promising target in the development of new therapies for cancer. The present study aimed to investigate the role of everolimus at 100 nM as inductor of the EMT phenomenon in cell lines derived from human breast (BT-549), colorectal (RKO-AS45-1) and ovary (TOV-21G) cancer. The integrity of cellular junctions was monitored using an in vitro model of epithelial resistance. The results demonstrated that the EMT genes ZEB1, TWIST1 and TGFB1 were differentially expressed in cells treated with everolimus compared with in untreated cells. lncRNA HOTAIR was upregulated post-treatment only in BT-549 cells compared with in untreated cells. After treatment with everolimus, the intensity of fluorescence of P-cadherin decreased, and that of fibronectin increased in RKO-AS45-1 and TOV-21G cells compared with control cells. The transepithelial electrical resistance at the RKO-AS45-1 monolayer treated with everolimus started to decrease at 48 h. The changes in the gene expression and epithelial resistance may confirm the role of everolimus in EMT.Ezequiel Dias Fdn, Cellular Biol Res & Dev Dept, BR-30510010 Belo Horizonte, MG, BrazilGrp Oncoclin, Hematol & Oncol Nucleus, BR-30140001 Belo Horizonte, MG, BrazilSao Paulo State Univ, Sch Med, Dept Obstet & Gynecol, BR-18618687 Botucatu, SP, BrazilEzequiel Dias Fdn, Pharmaceut Res & Dev, BR-30510010 Belo Horizonte, MG, BrazilSao Paulo State Univ, Sch Med, Dept Obstet & Gynecol, BR-18618687 Botucatu, SP, BrazilSpandidos Publ LtdEzequiel Dias FdnGrp OncoclinUniversidade Estadual Paulista (Unesp)Goncalves, Bryan Ortero PerezDe Andrade, Warne Pedro [UNESP]Da Conceicao Braga, LeticiaFialho, Silvia LigorioSilva, Luciana Maria2021-06-25T12:25:26Z2021-06-25T12:25:26Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.3892/ol.2020.12019Oncology Letters. Athens: Spandidos Publ Ltd, v. 20, n. 5, 11 p., 2020.1792-1074http://hdl.handle.net/11449/20966610.3892/ol.2020.12019WOS:000590173300035Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOncology Lettersinfo:eu-repo/semantics/openAccess2024-08-16T14:13:02Zoai:repositorio.unesp.br:11449/209666Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:13:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment |
title |
Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment |
spellingShingle |
Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment Goncalves, Bryan Ortero Perez EMT cancer everolimus HOTAIR biomarker |
title_short |
Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment |
title_full |
Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment |
title_fullStr |
Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment |
title_full_unstemmed |
Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment |
title_sort |
Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment |
author |
Goncalves, Bryan Ortero Perez |
author_facet |
Goncalves, Bryan Ortero Perez De Andrade, Warne Pedro [UNESP] Da Conceicao Braga, Leticia Fialho, Silvia Ligorio Silva, Luciana Maria |
author_role |
author |
author2 |
De Andrade, Warne Pedro [UNESP] Da Conceicao Braga, Leticia Fialho, Silvia Ligorio Silva, Luciana Maria |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Ezequiel Dias Fdn Grp Oncoclin Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Goncalves, Bryan Ortero Perez De Andrade, Warne Pedro [UNESP] Da Conceicao Braga, Leticia Fialho, Silvia Ligorio Silva, Luciana Maria |
dc.subject.por.fl_str_mv |
EMT cancer everolimus HOTAIR biomarker |
topic |
EMT cancer everolimus HOTAIR biomarker |
description |
The epithelial-to-mesenchymal transition (EMT) is a phenomenon during which cancer epithelial cells undergo changes in plasticity and lose cell-cell adhesion with consequent remodeling of the extracellular matrix and development of mesenchymal characteristics. Long non-coding RNAs (lncRNAs) have been described as EMT modulation markers, becoming a promising target in the development of new therapies for cancer. The present study aimed to investigate the role of everolimus at 100 nM as inductor of the EMT phenomenon in cell lines derived from human breast (BT-549), colorectal (RKO-AS45-1) and ovary (TOV-21G) cancer. The integrity of cellular junctions was monitored using an in vitro model of epithelial resistance. The results demonstrated that the EMT genes ZEB1, TWIST1 and TGFB1 were differentially expressed in cells treated with everolimus compared with in untreated cells. lncRNA HOTAIR was upregulated post-treatment only in BT-549 cells compared with in untreated cells. After treatment with everolimus, the intensity of fluorescence of P-cadherin decreased, and that of fibronectin increased in RKO-AS45-1 and TOV-21G cells compared with control cells. The transepithelial electrical resistance at the RKO-AS45-1 monolayer treated with everolimus started to decrease at 48 h. The changes in the gene expression and epithelial resistance may confirm the role of everolimus in EMT. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-01 2021-06-25T12:25:26Z 2021-06-25T12:25:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3892/ol.2020.12019 Oncology Letters. Athens: Spandidos Publ Ltd, v. 20, n. 5, 11 p., 2020. 1792-1074 http://hdl.handle.net/11449/209666 10.3892/ol.2020.12019 WOS:000590173300035 |
url |
http://dx.doi.org/10.3892/ol.2020.12019 http://hdl.handle.net/11449/209666 |
identifier_str_mv |
Oncology Letters. Athens: Spandidos Publ Ltd, v. 20, n. 5, 11 p., 2020. 1792-1074 10.3892/ol.2020.12019 WOS:000590173300035 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oncology Letters |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Spandidos Publ Ltd |
publisher.none.fl_str_mv |
Spandidos Publ Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128213271969792 |