Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment

Detalhes bibliográficos
Autor(a) principal: Goncalves, Bryan Ortero Perez
Data de Publicação: 2020
Outros Autores: De Andrade, Warne Pedro [UNESP], Da Conceicao Braga, Leticia, Fialho, Silvia Ligorio, Silva, Luciana Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3892/ol.2020.12019
http://hdl.handle.net/11449/209666
Resumo: The epithelial-to-mesenchymal transition (EMT) is a phenomenon during which cancer epithelial cells undergo changes in plasticity and lose cell-cell adhesion with consequent remodeling of the extracellular matrix and development of mesenchymal characteristics. Long non-coding RNAs (lncRNAs) have been described as EMT modulation markers, becoming a promising target in the development of new therapies for cancer. The present study aimed to investigate the role of everolimus at 100 nM as inductor of the EMT phenomenon in cell lines derived from human breast (BT-549), colorectal (RKO-AS45-1) and ovary (TOV-21G) cancer. The integrity of cellular junctions was monitored using an in vitro model of epithelial resistance. The results demonstrated that the EMT genes ZEB1, TWIST1 and TGFB1 were differentially expressed in cells treated with everolimus compared with in untreated cells. lncRNA HOTAIR was upregulated post-treatment only in BT-549 cells compared with in untreated cells. After treatment with everolimus, the intensity of fluorescence of P-cadherin decreased, and that of fibronectin increased in RKO-AS45-1 and TOV-21G cells compared with control cells. The transepithelial electrical resistance at the RKO-AS45-1 monolayer treated with everolimus started to decrease at 48 h. The changes in the gene expression and epithelial resistance may confirm the role of everolimus in EMT.
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spelling Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatmentEMTcancereverolimusHOTAIRbiomarkerThe epithelial-to-mesenchymal transition (EMT) is a phenomenon during which cancer epithelial cells undergo changes in plasticity and lose cell-cell adhesion with consequent remodeling of the extracellular matrix and development of mesenchymal characteristics. Long non-coding RNAs (lncRNAs) have been described as EMT modulation markers, becoming a promising target in the development of new therapies for cancer. The present study aimed to investigate the role of everolimus at 100 nM as inductor of the EMT phenomenon in cell lines derived from human breast (BT-549), colorectal (RKO-AS45-1) and ovary (TOV-21G) cancer. The integrity of cellular junctions was monitored using an in vitro model of epithelial resistance. The results demonstrated that the EMT genes ZEB1, TWIST1 and TGFB1 were differentially expressed in cells treated with everolimus compared with in untreated cells. lncRNA HOTAIR was upregulated post-treatment only in BT-549 cells compared with in untreated cells. After treatment with everolimus, the intensity of fluorescence of P-cadherin decreased, and that of fibronectin increased in RKO-AS45-1 and TOV-21G cells compared with control cells. The transepithelial electrical resistance at the RKO-AS45-1 monolayer treated with everolimus started to decrease at 48 h. The changes in the gene expression and epithelial resistance may confirm the role of everolimus in EMT.Ezequiel Dias Fdn, Cellular Biol Res & Dev Dept, BR-30510010 Belo Horizonte, MG, BrazilGrp Oncoclin, Hematol & Oncol Nucleus, BR-30140001 Belo Horizonte, MG, BrazilSao Paulo State Univ, Sch Med, Dept Obstet & Gynecol, BR-18618687 Botucatu, SP, BrazilEzequiel Dias Fdn, Pharmaceut Res & Dev, BR-30510010 Belo Horizonte, MG, BrazilSao Paulo State Univ, Sch Med, Dept Obstet & Gynecol, BR-18618687 Botucatu, SP, BrazilSpandidos Publ LtdEzequiel Dias FdnGrp OncoclinUniversidade Estadual Paulista (Unesp)Goncalves, Bryan Ortero PerezDe Andrade, Warne Pedro [UNESP]Da Conceicao Braga, LeticiaFialho, Silvia LigorioSilva, Luciana Maria2021-06-25T12:25:26Z2021-06-25T12:25:26Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.3892/ol.2020.12019Oncology Letters. Athens: Spandidos Publ Ltd, v. 20, n. 5, 11 p., 2020.1792-1074http://hdl.handle.net/11449/20966610.3892/ol.2020.12019WOS:000590173300035Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOncology Lettersinfo:eu-repo/semantics/openAccess2024-08-16T14:13:02Zoai:repositorio.unesp.br:11449/209666Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:13:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment
title Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment
spellingShingle Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment
Goncalves, Bryan Ortero Perez
EMT
cancer
everolimus
HOTAIR
biomarker
title_short Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment
title_full Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment
title_fullStr Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment
title_full_unstemmed Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment
title_sort Epithelial-to-mesenchymal transition markers are differentially expressed in epithelial cancer cell lines after everolimus treatment
author Goncalves, Bryan Ortero Perez
author_facet Goncalves, Bryan Ortero Perez
De Andrade, Warne Pedro [UNESP]
Da Conceicao Braga, Leticia
Fialho, Silvia Ligorio
Silva, Luciana Maria
author_role author
author2 De Andrade, Warne Pedro [UNESP]
Da Conceicao Braga, Leticia
Fialho, Silvia Ligorio
Silva, Luciana Maria
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ezequiel Dias Fdn
Grp Oncoclin
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Goncalves, Bryan Ortero Perez
De Andrade, Warne Pedro [UNESP]
Da Conceicao Braga, Leticia
Fialho, Silvia Ligorio
Silva, Luciana Maria
dc.subject.por.fl_str_mv EMT
cancer
everolimus
HOTAIR
biomarker
topic EMT
cancer
everolimus
HOTAIR
biomarker
description The epithelial-to-mesenchymal transition (EMT) is a phenomenon during which cancer epithelial cells undergo changes in plasticity and lose cell-cell adhesion with consequent remodeling of the extracellular matrix and development of mesenchymal characteristics. Long non-coding RNAs (lncRNAs) have been described as EMT modulation markers, becoming a promising target in the development of new therapies for cancer. The present study aimed to investigate the role of everolimus at 100 nM as inductor of the EMT phenomenon in cell lines derived from human breast (BT-549), colorectal (RKO-AS45-1) and ovary (TOV-21G) cancer. The integrity of cellular junctions was monitored using an in vitro model of epithelial resistance. The results demonstrated that the EMT genes ZEB1, TWIST1 and TGFB1 were differentially expressed in cells treated with everolimus compared with in untreated cells. lncRNA HOTAIR was upregulated post-treatment only in BT-549 cells compared with in untreated cells. After treatment with everolimus, the intensity of fluorescence of P-cadherin decreased, and that of fibronectin increased in RKO-AS45-1 and TOV-21G cells compared with control cells. The transepithelial electrical resistance at the RKO-AS45-1 monolayer treated with everolimus started to decrease at 48 h. The changes in the gene expression and epithelial resistance may confirm the role of everolimus in EMT.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-01
2021-06-25T12:25:26Z
2021-06-25T12:25:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3892/ol.2020.12019
Oncology Letters. Athens: Spandidos Publ Ltd, v. 20, n. 5, 11 p., 2020.
1792-1074
http://hdl.handle.net/11449/209666
10.3892/ol.2020.12019
WOS:000590173300035
url http://dx.doi.org/10.3892/ol.2020.12019
http://hdl.handle.net/11449/209666
identifier_str_mv Oncology Letters. Athens: Spandidos Publ Ltd, v. 20, n. 5, 11 p., 2020.
1792-1074
10.3892/ol.2020.12019
WOS:000590173300035
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Oncology Letters
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
dc.publisher.none.fl_str_mv Spandidos Publ Ltd
publisher.none.fl_str_mv Spandidos Publ Ltd
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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