Análise imunoistoquímica no tecido ósseo alveolar da influência da cisplatina na periodontite experimental em ratos

• Autor(a) principal: Fernandes, Giovani Lopes [UNESP]
• Data de Publicação: 2018
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/204038; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2019-05-29/000914687.pdf
• topic: Periodontite; Quimioterapia; Ratos; Cisplatina; Periodontitis

The aim of this animal study evaluate via photometric analysis by micro-computed tomography (Micro-CT) and analysis of immunohistochemically study, the influence of chemotherapeutic drug Cisplatin (CIS) on metabolism of alveolar bone in the evolution of experimental periodontitis (EP) in rats. In the present study, 120 male rats divided following a table generated by a computer program into 4 groups, with 30 animals each. NEP- SS group, also called the sham group. The animals in this group received two intraperitoneal injections with 48-hour interval between them of 0.5 ml of saline solution 0.9 % (SS). These animals were not induced to EP. EP - SS group: the animals in this group received two intraperitoneal injections with 48-hour interval between them of 0.5 ml of SS. Soon after the first injection, PE was induced in the lower first right and left molars. NEP - CIS group: the animals in this group received two intraperitoneal injections with 48- hour interval between them of 5 mg/kg and 2.5 mg/kg of cisplatin (CIS) respectively. These animals were not induced to EP. EP - CIS group: the animals in this group received two intraperitoneal injections with 48-hour interval between them of 5 mg/kg and 2.5 mg/kg CIS respectively. Soon after the first injection, PE was induced in the lower first right and left molars. EP was induced by the placement of a number 24 cotton thread around the lower first molars. After of 07, 15 and 30 days after the first intraperitoneal injection (SS or chemotherapy) animals were euthanized using an overdose of thiopental (150mg/kg). The mandibles collected were divided into two parts and processed according each analyzes. For the photometric analysis was evaluated bone loss (EBL) in the first molar region. For analysis by Micro-CT was evaluated the percentage of bone volume (PBV) in the first molar furcation area. The contralateral mandibles...