Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.biomedcentral.com/1471-2180/14/299 http://hdl.handle.net/11449/129969 |
Resumo: | Background: Attachment is essential to maintain bacteria at their preferential intestinal colonization sites. There is little information on the influence of different environmental conditions in the interaction of atypical enteropathogenic Escherichia coli (aEPEC) strains with epithelial cells. In this study, we evaluated the effect of different glucose (5 and 25 mM) and CO2 (0.03 and 5%) concentrations and presence of bile salts on the adhesiveness of the aEPEC strain 1551-2.Results: We found that a CO2-enriched atmosphere enhanced the adhesiveness of the aEPEC 1551-2 strain independently of glucose concentrations or presence of bile salts. Conversely, the presence of high glucose concentration altered the original localized adherence (LA) pattern observed at 5 mM glucose, which is characterized by the formation of compact bacterial clusters, to a hybrid adherence pattern (LA and an aggregative adherence-like pattern). In addition, at high glucose concentration, there was increased expression of the fimA gene, which encodes the major subunit of type 1 pilus (T1P), and an isogenic fimA mutant displayed only LA. The presence of bile salts did not interfere with the adhesion properties of the 1551-2 strain to HeLa cells.Conclusions: Our data suggest that a CO2-enriched atmosphere could favor aEPEC adhesion to the host cells, whereas enhanced T1P production under high glucose concentration could allow bacteria to access more extensive intestinal colonization sites in the host at the beginning of the infectious process. |
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Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cellsAtypical EPECGlucoseType I pilusAdherenceEnvironmental factorsDiarrheaBackground: Attachment is essential to maintain bacteria at their preferential intestinal colonization sites. There is little information on the influence of different environmental conditions in the interaction of atypical enteropathogenic Escherichia coli (aEPEC) strains with epithelial cells. In this study, we evaluated the effect of different glucose (5 and 25 mM) and CO2 (0.03 and 5%) concentrations and presence of bile salts on the adhesiveness of the aEPEC strain 1551-2.Results: We found that a CO2-enriched atmosphere enhanced the adhesiveness of the aEPEC 1551-2 strain independently of glucose concentrations or presence of bile salts. Conversely, the presence of high glucose concentration altered the original localized adherence (LA) pattern observed at 5 mM glucose, which is characterized by the formation of compact bacterial clusters, to a hybrid adherence pattern (LA and an aggregative adherence-like pattern). In addition, at high glucose concentration, there was increased expression of the fimA gene, which encodes the major subunit of type 1 pilus (T1P), and an isogenic fimA mutant displayed only LA. The presence of bile salts did not interfere with the adhesion properties of the 1551-2 strain to HeLa cells.Conclusions: Our data suggest that a CO2-enriched atmosphere could favor aEPEC adhesion to the host cells, whereas enhanced T1P production under high glucose concentration could allow bacteria to access more extensive intestinal colonization sites in the host at the beginning of the infectious process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Sao Paulo UNIFESP, Dept Microbiol Imunol &Parasitol, Escola Paulista Med, Sao Paulo, BrazilUniv Estadual Paulista UNESP, Dept Microbiol &Imunol, Inst Biociencias, Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Dept Microbiol & Imunol, Inst Biociencias, Botucatu, SP, BrazilFAPESP: 2011/12664-5CNPq: 304453/2011-0CNPq: 480664/2012-9Biomed Central LtdUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Romao, Fabiano T.Hernandes, Rodrigo T. [UNESP]Yamamoto, DeniseOsugui, LikaPopi, Ana FlaviaGomes, Tania A. T.2015-11-03T15:28:16Z2015-11-03T15:28:16Z2014-12-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7application/pdfhttp://www.biomedcentral.com/1471-2180/14/299Bmc Microbiology. London: Biomed Central Ltd, v. 14, p. 1-7, 2014.1471-2180http://hdl.handle.net/11449/12996910.1186/s12866-014-0299-yWOS:000348166000001WOS000348166000001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Microbiology2.8291,242info:eu-repo/semantics/openAccess2023-10-18T06:08:52Zoai:repositorio.unesp.br:11449/129969Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-18T06:08:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells |
title |
Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells |
spellingShingle |
Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells Romao, Fabiano T. Atypical EPEC Glucose Type I pilus Adherence Environmental factors Diarrhea |
title_short |
Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells |
title_full |
Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells |
title_fullStr |
Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells |
title_full_unstemmed |
Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells |
title_sort |
Influence of environmental factors in the adherence of an atypical enteropathogenic Escherichia coli strain to epithelial cells |
author |
Romao, Fabiano T. |
author_facet |
Romao, Fabiano T. Hernandes, Rodrigo T. [UNESP] Yamamoto, Denise Osugui, Lika Popi, Ana Flavia Gomes, Tania A. T. |
author_role |
author |
author2 |
Hernandes, Rodrigo T. [UNESP] Yamamoto, Denise Osugui, Lika Popi, Ana Flavia Gomes, Tania A. T. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Romao, Fabiano T. Hernandes, Rodrigo T. [UNESP] Yamamoto, Denise Osugui, Lika Popi, Ana Flavia Gomes, Tania A. T. |
dc.subject.por.fl_str_mv |
Atypical EPEC Glucose Type I pilus Adherence Environmental factors Diarrhea |
topic |
Atypical EPEC Glucose Type I pilus Adherence Environmental factors Diarrhea |
description |
Background: Attachment is essential to maintain bacteria at their preferential intestinal colonization sites. There is little information on the influence of different environmental conditions in the interaction of atypical enteropathogenic Escherichia coli (aEPEC) strains with epithelial cells. In this study, we evaluated the effect of different glucose (5 and 25 mM) and CO2 (0.03 and 5%) concentrations and presence of bile salts on the adhesiveness of the aEPEC strain 1551-2.Results: We found that a CO2-enriched atmosphere enhanced the adhesiveness of the aEPEC 1551-2 strain independently of glucose concentrations or presence of bile salts. Conversely, the presence of high glucose concentration altered the original localized adherence (LA) pattern observed at 5 mM glucose, which is characterized by the formation of compact bacterial clusters, to a hybrid adherence pattern (LA and an aggregative adherence-like pattern). In addition, at high glucose concentration, there was increased expression of the fimA gene, which encodes the major subunit of type 1 pilus (T1P), and an isogenic fimA mutant displayed only LA. The presence of bile salts did not interfere with the adhesion properties of the 1551-2 strain to HeLa cells.Conclusions: Our data suggest that a CO2-enriched atmosphere could favor aEPEC adhesion to the host cells, whereas enhanced T1P production under high glucose concentration could allow bacteria to access more extensive intestinal colonization sites in the host at the beginning of the infectious process. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-20 2015-11-03T15:28:16Z 2015-11-03T15:28:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.biomedcentral.com/1471-2180/14/299 Bmc Microbiology. London: Biomed Central Ltd, v. 14, p. 1-7, 2014. 1471-2180 http://hdl.handle.net/11449/129969 10.1186/s12866-014-0299-y WOS:000348166000001 WOS000348166000001.pdf |
url |
http://www.biomedcentral.com/1471-2180/14/299 http://hdl.handle.net/11449/129969 |
identifier_str_mv |
Bmc Microbiology. London: Biomed Central Ltd, v. 14, p. 1-7, 2014. 1471-2180 10.1186/s12866-014-0299-y WOS:000348166000001 WOS000348166000001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Microbiology 2.829 1,242 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-7 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd |
publisher.none.fl_str_mv |
Biomed Central Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789435121631232 |