Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner

Detalhes bibliográficos
Autor(a) principal: Cavariani, Marilia Martins [UNESP]
Data de Publicação: 2022
Outros Autores: de Mello Santos, Talita [UNESP], Chuffa, Luiz Gustavo de Almeida [UNESP], Pinheiro, Patrícia Fernanda Felipe [UNESP], Scarano, Wellerson Rodrigo [UNESP], Domeniconi, Raquel Fantin [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fcell.2022.816637
http://hdl.handle.net/11449/241802
Resumo: Nutrition is an environmental factor able to activate physiological interactions between fetus and mother. Maternal protein restriction is able to alter sperm parameters associated with epididymal functions. Since correct development and functioning of the epididymides are fundamental for mammalian reproductive success, this study investigated the effects of maternal protein restriction on epididymal morphology and morphometry in rat offspring as well as on the expression of Src, Cldn-1, AR, ER, aromatase p450, and 5α-reductase in different stages of postnatal epididymal development. For this purpose, pregnant females were allocated to normal-protein (NP—17% protein) and low-protein (LP—6% protein) groups that received specific diets during gestation and lactation. After weaning, male offspring was provided only normal-protein diet until the ages of 21, 44, and 120 days, when they were euthanized and their epididymides collected. Maternal protein restriction decreased genital organs weight as well as crown-rump length and anogenital distance at all ages. Although the low-protein diet did not change the integrity of the epididymal epithelium, we observed decreases in tubular diameter, epithelial height and luminal diameter of the epididymal duct in 21-day-old LP animals. The maternal low-protein diet changed AR, ERα, ERβ, Src 416, and Src 527 expression in offspring epididymides in an age-dependent manner. Finally, maternal protein restriction increased Cldn-1 expression throughout the epididymides at all analyzed ages. Although some of these changes did not remain until adulthood, the insufficient supply of proteins in early life altered the structure and functioning of the epididymis in important periods of postnatal development.
id UNSP_8a7f2960fa31d14bf3a595b3b58f2af0
oai_identifier_str oai:repositorio.unesp.br:11449/241802
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent MannerCLDN-1epididymishormone receptorsmaternal protein restrictionpostnatal developmentsrcNutrition is an environmental factor able to activate physiological interactions between fetus and mother. Maternal protein restriction is able to alter sperm parameters associated with epididymal functions. Since correct development and functioning of the epididymides are fundamental for mammalian reproductive success, this study investigated the effects of maternal protein restriction on epididymal morphology and morphometry in rat offspring as well as on the expression of Src, Cldn-1, AR, ER, aromatase p450, and 5α-reductase in different stages of postnatal epididymal development. For this purpose, pregnant females were allocated to normal-protein (NP—17% protein) and low-protein (LP—6% protein) groups that received specific diets during gestation and lactation. After weaning, male offspring was provided only normal-protein diet until the ages of 21, 44, and 120 days, when they were euthanized and their epididymides collected. Maternal protein restriction decreased genital organs weight as well as crown-rump length and anogenital distance at all ages. Although the low-protein diet did not change the integrity of the epididymal epithelium, we observed decreases in tubular diameter, epithelial height and luminal diameter of the epididymal duct in 21-day-old LP animals. The maternal low-protein diet changed AR, ERα, ERβ, Src 416, and Src 527 expression in offspring epididymides in an age-dependent manner. Finally, maternal protein restriction increased Cldn-1 expression throughout the epididymides at all analyzed ages. Although some of these changes did not remain until adulthood, the insufficient supply of proteins in early life altered the structure and functioning of the epididymis in important periods of postnatal development.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Department of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)Cavariani, Marilia Martins [UNESP]de Mello Santos, Talita [UNESP]Chuffa, Luiz Gustavo de Almeida [UNESP]Pinheiro, Patrícia Fernanda Felipe [UNESP]Scarano, Wellerson Rodrigo [UNESP]Domeniconi, Raquel Fantin [UNESP]2023-03-02T00:28:25Z2023-03-02T00:28:25Z2022-04-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fcell.2022.816637Frontiers in Cell and Developmental Biology, v. 10.2296-634Xhttp://hdl.handle.net/11449/24180210.3389/fcell.2022.8166372-s2.0-85129511539Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Cell and Developmental Biologyinfo:eu-repo/semantics/openAccess2023-03-02T00:28:25Zoai:repositorio.unesp.br:11449/241802Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:15:29.251454Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner
title Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner
spellingShingle Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner
Cavariani, Marilia Martins [UNESP]
CLDN-1
epididymis
hormone receptors
maternal protein restriction
postnatal development
src
title_short Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner
title_full Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner
title_fullStr Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner
title_full_unstemmed Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner
title_sort Maternal Protein Restriction Alters the Expression of Proteins Related to the Structure and Functioning of the Rat Offspring Epididymis in an Age-Dependent Manner
author Cavariani, Marilia Martins [UNESP]
author_facet Cavariani, Marilia Martins [UNESP]
de Mello Santos, Talita [UNESP]
Chuffa, Luiz Gustavo de Almeida [UNESP]
Pinheiro, Patrícia Fernanda Felipe [UNESP]
Scarano, Wellerson Rodrigo [UNESP]
Domeniconi, Raquel Fantin [UNESP]
author_role author
author2 de Mello Santos, Talita [UNESP]
Chuffa, Luiz Gustavo de Almeida [UNESP]
Pinheiro, Patrícia Fernanda Felipe [UNESP]
Scarano, Wellerson Rodrigo [UNESP]
Domeniconi, Raquel Fantin [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Cavariani, Marilia Martins [UNESP]
de Mello Santos, Talita [UNESP]
Chuffa, Luiz Gustavo de Almeida [UNESP]
Pinheiro, Patrícia Fernanda Felipe [UNESP]
Scarano, Wellerson Rodrigo [UNESP]
Domeniconi, Raquel Fantin [UNESP]
dc.subject.por.fl_str_mv CLDN-1
epididymis
hormone receptors
maternal protein restriction
postnatal development
src
topic CLDN-1
epididymis
hormone receptors
maternal protein restriction
postnatal development
src
description Nutrition is an environmental factor able to activate physiological interactions between fetus and mother. Maternal protein restriction is able to alter sperm parameters associated with epididymal functions. Since correct development and functioning of the epididymides are fundamental for mammalian reproductive success, this study investigated the effects of maternal protein restriction on epididymal morphology and morphometry in rat offspring as well as on the expression of Src, Cldn-1, AR, ER, aromatase p450, and 5α-reductase in different stages of postnatal epididymal development. For this purpose, pregnant females were allocated to normal-protein (NP—17% protein) and low-protein (LP—6% protein) groups that received specific diets during gestation and lactation. After weaning, male offspring was provided only normal-protein diet until the ages of 21, 44, and 120 days, when they were euthanized and their epididymides collected. Maternal protein restriction decreased genital organs weight as well as crown-rump length and anogenital distance at all ages. Although the low-protein diet did not change the integrity of the epididymal epithelium, we observed decreases in tubular diameter, epithelial height and luminal diameter of the epididymal duct in 21-day-old LP animals. The maternal low-protein diet changed AR, ERα, ERβ, Src 416, and Src 527 expression in offspring epididymides in an age-dependent manner. Finally, maternal protein restriction increased Cldn-1 expression throughout the epididymides at all analyzed ages. Although some of these changes did not remain until adulthood, the insufficient supply of proteins in early life altered the structure and functioning of the epididymis in important periods of postnatal development.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-19
2023-03-02T00:28:25Z
2023-03-02T00:28:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fcell.2022.816637
Frontiers in Cell and Developmental Biology, v. 10.
2296-634X
http://hdl.handle.net/11449/241802
10.3389/fcell.2022.816637
2-s2.0-85129511539
url http://dx.doi.org/10.3389/fcell.2022.816637
http://hdl.handle.net/11449/241802
identifier_str_mv Frontiers in Cell and Developmental Biology, v. 10.
2296-634X
10.3389/fcell.2022.816637
2-s2.0-85129511539
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Cell and Developmental Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129301734752256