Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/vol7-3gmr462 http://hdl.handle.net/11449/10807 |
Resumo: | Plants are a source of many biologically active products and nowadays they are of great interest to the pharmaceutical industry. In the present study, the mutagenic potential of the Musa paradisiaca fruit peel extract was assessed by the single-cell gel electrophoresis (SCGE) and micronucleus assays. Animals were treated orally with three different concentrations of the extract (1000, 1500, and 2000 mg/kg body weight). Peripheral blood cells of Swiss mice were collected 24 h after treatment for the SCGE assay and 48 and 72 h for the micronucleus test. The results showed that the two higher doses of the extract of M. paradisiaca induced statistically significant increases in the average numbers of DNA damage in peripheral blood leukocytes for the two higher doses and a significant increase in the mean of micronucleated polychromatic erythrocytes in the three doses tested. The polychromatic/normochromatic erythrocyte ratio scored in the treated groups was not statistically different from the negative control. The data obtained indicate that fruit peel extract from M. paradisiaca showed mutagenic effect in the peripheral blood cells of Swiss albino mice. |
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Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivoMusa paradisiacaSingle-cell gel electrophoresisMicronucleus testPeripheral blood cellsComet assayPlants are a source of many biologically active products and nowadays they are of great interest to the pharmaceutical industry. In the present study, the mutagenic potential of the Musa paradisiaca fruit peel extract was assessed by the single-cell gel electrophoresis (SCGE) and micronucleus assays. Animals were treated orally with three different concentrations of the extract (1000, 1500, and 2000 mg/kg body weight). Peripheral blood cells of Swiss mice were collected 24 h after treatment for the SCGE assay and 48 and 72 h for the micronucleus test. The results showed that the two higher doses of the extract of M. paradisiaca induced statistically significant increases in the average numbers of DNA damage in peripheral blood leukocytes for the two higher doses and a significant increase in the mean of micronucleated polychromatic erythrocytes in the three doses tested. The polychromatic/normochromatic erythrocyte ratio scored in the treated groups was not statistically different from the negative control. The data obtained indicate that fruit peel extract from M. paradisiaca showed mutagenic effect in the peripheral blood cells of Swiss albino mice.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Univ Estadual Paulista, Dept Fonoaudiol, Fac Filosofia & Ciencias, Marilia, SP, BrazilUniv Fed Amapa, Lab Farmacos, Macapa, PA, BrazilUniv Alfenas, Fac Enfermagem, Alfenas, MG, BrazilUniv Estadual Paulista, Dept Fonoaudiol, Fac Filosofia & Ciencias, Marilia, SP, BrazilCNPq: 306544/2006-7FAPESP: 06/57514-2FAPEMIG: EDT - 1879/02Funpec-editoraUniversidade Estadual Paulista (Unesp)Universidade Federal do Amapá (UNIFAP)Univ AlfenasAndrade, C. U. B.Perazzo, F. F.Maistro, Edson Luis [UNESP]2014-05-20T13:31:44Z2014-05-20T13:31:44Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article725-732application/pdfhttp://dx.doi.org/10.4238/vol7-3gmr462Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 7, n. 3, p. 725-732, 2008.1676-5680http://hdl.handle.net/11449/1080710.4238/vol7-3gmr462WOS:000260227100016WOS000260227100016.pdf47875216130383150000-0003-0757-7876Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2023-12-20T06:18:21Zoai:repositorio.unesp.br:11449/10807Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-20T06:18:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo |
title |
Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo |
spellingShingle |
Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo Andrade, C. U. B. Musa paradisiaca Single-cell gel electrophoresis Micronucleus test Peripheral blood cells Comet assay |
title_short |
Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo |
title_full |
Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo |
title_fullStr |
Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo |
title_full_unstemmed |
Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo |
title_sort |
Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo |
author |
Andrade, C. U. B. |
author_facet |
Andrade, C. U. B. Perazzo, F. F. Maistro, Edson Luis [UNESP] |
author_role |
author |
author2 |
Perazzo, F. F. Maistro, Edson Luis [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal do Amapá (UNIFAP) Univ Alfenas |
dc.contributor.author.fl_str_mv |
Andrade, C. U. B. Perazzo, F. F. Maistro, Edson Luis [UNESP] |
dc.subject.por.fl_str_mv |
Musa paradisiaca Single-cell gel electrophoresis Micronucleus test Peripheral blood cells Comet assay |
topic |
Musa paradisiaca Single-cell gel electrophoresis Micronucleus test Peripheral blood cells Comet assay |
description |
Plants are a source of many biologically active products and nowadays they are of great interest to the pharmaceutical industry. In the present study, the mutagenic potential of the Musa paradisiaca fruit peel extract was assessed by the single-cell gel electrophoresis (SCGE) and micronucleus assays. Animals were treated orally with three different concentrations of the extract (1000, 1500, and 2000 mg/kg body weight). Peripheral blood cells of Swiss mice were collected 24 h after treatment for the SCGE assay and 48 and 72 h for the micronucleus test. The results showed that the two higher doses of the extract of M. paradisiaca induced statistically significant increases in the average numbers of DNA damage in peripheral blood leukocytes for the two higher doses and a significant increase in the mean of micronucleated polychromatic erythrocytes in the three doses tested. The polychromatic/normochromatic erythrocyte ratio scored in the treated groups was not statistically different from the negative control. The data obtained indicate that fruit peel extract from M. paradisiaca showed mutagenic effect in the peripheral blood cells of Swiss albino mice. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-01 2014-05-20T13:31:44Z 2014-05-20T13:31:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/vol7-3gmr462 Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 7, n. 3, p. 725-732, 2008. 1676-5680 http://hdl.handle.net/11449/10807 10.4238/vol7-3gmr462 WOS:000260227100016 WOS000260227100016.pdf 4787521613038315 0000-0003-0757-7876 |
url |
http://dx.doi.org/10.4238/vol7-3gmr462 http://hdl.handle.net/11449/10807 |
identifier_str_mv |
Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 7, n. 3, p. 725-732, 2008. 1676-5680 10.4238/vol7-3gmr462 WOS:000260227100016 WOS000260227100016.pdf 4787521613038315 0000-0003-0757-7876 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
725-732 application/pdf |
dc.publisher.none.fl_str_mv |
Funpec-editora |
publisher.none.fl_str_mv |
Funpec-editora |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965342850613248 |