Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2012.03.003 http://hdl.handle.net/11449/14702 |
Resumo: | The objectives were to evaluate the reexpansion blastocoele rate, post-thaw viability, and in vitro development of canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol (GLY) or 1.5 m ethylene glycol (EG). Fifty-one in vivo-produced canine blastocysts were randomly allocated in two groups: GLY (n = 26) and EG (n = 25). After thawing, embryos from MO were immediately stained with the fluorescent probes propidium iodide and Hoechst 33 342 to evaluate cellular viability. Frozen-thawed embryos from M3 and M6 were cultured in SOFaa medium + 10% FCS at 38.5 degrees C under an atmosphere of 5% CO2 with maximum humidity, for 3 and 6 days, respectively, and similarly stained. The blastocoele reexpansion rate (24 h after in vitro culture) did not differ between GLY (76.5%) and EG (68.8%). Post-thaw viable cells rate were not significantly different between GLY and EG (66.5 +/- 4.8 and 57.3 +/- 4.8, respectively, mean +/- SEM), or among MO (62.3 +/- 5.7%), M3 (56.9 +/- 6.0%), and M6 (66.5 +/- 6.0%). In conclusion, canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol or 1.5 m ethylene glycol, had satisfactory blastocoele reexpansion rates, similar post-thawing viability, and remained viable for up to 6 days of in vitro culture. (C) 2012 Elsevier B.V. All rights reserved. |
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Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezingCryopreservationEmbryo viabilityBlastocoele reexpansionGlycerolEthylene glycolDogThe objectives were to evaluate the reexpansion blastocoele rate, post-thaw viability, and in vitro development of canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol (GLY) or 1.5 m ethylene glycol (EG). Fifty-one in vivo-produced canine blastocysts were randomly allocated in two groups: GLY (n = 26) and EG (n = 25). After thawing, embryos from MO were immediately stained with the fluorescent probes propidium iodide and Hoechst 33 342 to evaluate cellular viability. Frozen-thawed embryos from M3 and M6 were cultured in SOFaa medium + 10% FCS at 38.5 degrees C under an atmosphere of 5% CO2 with maximum humidity, for 3 and 6 days, respectively, and similarly stained. The blastocoele reexpansion rate (24 h after in vitro culture) did not differ between GLY (76.5%) and EG (68.8%). Post-thaw viable cells rate were not significantly different between GLY and EG (66.5 +/- 4.8 and 57.3 +/- 4.8, respectively, mean +/- SEM), or among MO (62.3 +/- 5.7%), M3 (56.9 +/- 6.0%), and M6 (66.5 +/- 6.0%). In conclusion, canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol or 1.5 m ethylene glycol, had satisfactory blastocoele reexpansion rates, similar post-thawing viability, and remained viable for up to 6 days of in vitro culture. (C) 2012 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal do Espírito Santo (UFES), Lab Anim Reprod, Dept Vet Med, BR-29500000 Alegre, ES, BrazilSão Paulo State Univ, UNESP, Sch Vet Med & Anim Sci, FMVZ,Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, BrazilUniversidade Federal de Minas Gerais (UFMG), Sch Vet, Dept Vet Clin & Surg, Sect Anim Reprod, BR-30123970 Belo Horizonte, MG, BrazilUniv São Paulo, Lab Hormonal Assays, Dept Anim Reprod, Fac Vet Med & Anim Sci, BR-05508270 São Paulo, BrazilSão Paulo State Univ, UNESP, Sch Vet Med & Anim Sci, FMVZ,Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, BrazilCNPq: 36600736/2007Elsevier B.V.Universidade Federal do Espírito Santo (UFES)Universidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)Universidade de São Paulo (USP)Guaitolini, C. Renato de Freitas [UNESP]Taffarel, M. Onghero [UNESP]Teixeira, N. SoaresSudano, M. Jose [UNESP]Freitas, P. Maria ColettoLopes, Maria Denise [UNESP]Landim-Alvarenga, Fernanda da Cruz [UNESP]Oliveira, C. Alvarenga deLuz, M. Rezende2013-09-30T18:29:07Z2014-05-20T13:42:18Z2013-09-30T18:29:07Z2014-05-20T13:42:18Z2012-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article576-582application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2012.03.003Theriogenology. New York: Elsevier B.V., v. 78, n. 3, p. 576-582, 2012.0093-691Xhttp://hdl.handle.net/11449/1470210.1016/j.theriogenology.2012.03.003WOS:000306538100011WOS000306538100011.pdf6666129914663018Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenology2.136info:eu-repo/semantics/openAccess2024-09-09T14:01:09Zoai:repositorio.unesp.br:11449/14702Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing |
title |
Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing |
spellingShingle |
Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing Guaitolini, C. Renato de Freitas [UNESP] Cryopreservation Embryo viability Blastocoele reexpansion Glycerol Ethylene glycol Dog |
title_short |
Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing |
title_full |
Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing |
title_fullStr |
Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing |
title_full_unstemmed |
Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing |
title_sort |
Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing |
author |
Guaitolini, C. Renato de Freitas [UNESP] |
author_facet |
Guaitolini, C. Renato de Freitas [UNESP] Taffarel, M. Onghero [UNESP] Teixeira, N. Soares Sudano, M. Jose [UNESP] Freitas, P. Maria Coletto Lopes, Maria Denise [UNESP] Landim-Alvarenga, Fernanda da Cruz [UNESP] Oliveira, C. Alvarenga de Luz, M. Rezende |
author_role |
author |
author2 |
Taffarel, M. Onghero [UNESP] Teixeira, N. Soares Sudano, M. Jose [UNESP] Freitas, P. Maria Coletto Lopes, Maria Denise [UNESP] Landim-Alvarenga, Fernanda da Cruz [UNESP] Oliveira, C. Alvarenga de Luz, M. Rezende |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Espírito Santo (UFES) Universidade Estadual Paulista (Unesp) Universidade Federal de Minas Gerais (UFMG) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Guaitolini, C. Renato de Freitas [UNESP] Taffarel, M. Onghero [UNESP] Teixeira, N. Soares Sudano, M. Jose [UNESP] Freitas, P. Maria Coletto Lopes, Maria Denise [UNESP] Landim-Alvarenga, Fernanda da Cruz [UNESP] Oliveira, C. Alvarenga de Luz, M. Rezende |
dc.subject.por.fl_str_mv |
Cryopreservation Embryo viability Blastocoele reexpansion Glycerol Ethylene glycol Dog |
topic |
Cryopreservation Embryo viability Blastocoele reexpansion Glycerol Ethylene glycol Dog |
description |
The objectives were to evaluate the reexpansion blastocoele rate, post-thaw viability, and in vitro development of canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol (GLY) or 1.5 m ethylene glycol (EG). Fifty-one in vivo-produced canine blastocysts were randomly allocated in two groups: GLY (n = 26) and EG (n = 25). After thawing, embryos from MO were immediately stained with the fluorescent probes propidium iodide and Hoechst 33 342 to evaluate cellular viability. Frozen-thawed embryos from M3 and M6 were cultured in SOFaa medium + 10% FCS at 38.5 degrees C under an atmosphere of 5% CO2 with maximum humidity, for 3 and 6 days, respectively, and similarly stained. The blastocoele reexpansion rate (24 h after in vitro culture) did not differ between GLY (76.5%) and EG (68.8%). Post-thaw viable cells rate were not significantly different between GLY and EG (66.5 +/- 4.8 and 57.3 +/- 4.8, respectively, mean +/- SEM), or among MO (62.3 +/- 5.7%), M3 (56.9 +/- 6.0%), and M6 (66.5 +/- 6.0%). In conclusion, canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol or 1.5 m ethylene glycol, had satisfactory blastocoele reexpansion rates, similar post-thawing viability, and remained viable for up to 6 days of in vitro culture. (C) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-01 2013-09-30T18:29:07Z 2013-09-30T18:29:07Z 2014-05-20T13:42:18Z 2014-05-20T13:42:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2012.03.003 Theriogenology. New York: Elsevier B.V., v. 78, n. 3, p. 576-582, 2012. 0093-691X http://hdl.handle.net/11449/14702 10.1016/j.theriogenology.2012.03.003 WOS:000306538100011 WOS000306538100011.pdf 6666129914663018 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2012.03.003 http://hdl.handle.net/11449/14702 |
identifier_str_mv |
Theriogenology. New York: Elsevier B.V., v. 78, n. 3, p. 576-582, 2012. 0093-691X 10.1016/j.theriogenology.2012.03.003 WOS:000306538100011 WOS000306538100011.pdf 6666129914663018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology 2.136 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
576-582 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021324424216576 |