Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2017/6589270 http://hdl.handle.net/11449/175243 |
Resumo: | The wound healing is a complex process which, sometimes, can be a problem in public health because of the possibility of physical disability or even death. Due to the lack of a gold standard drug in skin wound treatment and aiming at the discovery of new treatments in skin repair and the mechanisms involved in the process, we used oleoresin (OR) from Copaifera langsdorffii and hydroalcoholic extract of the leaves (EH) to treat rat skin wounds. For that, male Wistar rats were divided into groups (n=8): Lanette, Collagenase, 10% EH, or 10% OR and, after anesthesia, one wound of 2 cm was made in the back of animals. The wounds were treated once a day for 3, 7, or 14 days and the wound areas were measured. The rats were euthanized and skin samples destined to biochemical, molecular, and immunohistochemical analysis. The results showed a macroscopic retraction of the wounds of 10% EH and 10% OR creams and both treatments showed anti-inflammatory activity. Molecular and immunohistochemical results demonstrated the activity of Copaifera langsdorffii creams in angiogenesis, reepithelialization, wound retraction, and remodeling mechanisms. |
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Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in RatsThe wound healing is a complex process which, sometimes, can be a problem in public health because of the possibility of physical disability or even death. Due to the lack of a gold standard drug in skin wound treatment and aiming at the discovery of new treatments in skin repair and the mechanisms involved in the process, we used oleoresin (OR) from Copaifera langsdorffii and hydroalcoholic extract of the leaves (EH) to treat rat skin wounds. For that, male Wistar rats were divided into groups (n=8): Lanette, Collagenase, 10% EH, or 10% OR and, after anesthesia, one wound of 2 cm was made in the back of animals. The wounds were treated once a day for 3, 7, or 14 days and the wound areas were measured. The rats were euthanized and skin samples destined to biochemical, molecular, and immunohistochemical analysis. The results showed a macroscopic retraction of the wounds of 10% EH and 10% OR creams and both treatments showed anti-inflammatory activity. Molecular and immunohistochemical results demonstrated the activity of Copaifera langsdorffii creams in angiogenesis, reepithelialization, wound retraction, and remodeling mechanisms.Department of Morphology Institute of Biosciences of Botucatu UNESPDepartment of Surgery and Veterinary Anesthesiology School of Veterinary Medicine and Animal Science UNESPDepartment of Pharmaceutical Sciences School of Pharmaceutical Sciences of Ribeiraõ Preto University of Saõ Paulo Campus Ribeiraõ PretoDepartment of Biostatistics Institute of Biosciences of Botucatu UNESPDepartment of Morphology Institute of Biosciences of Botucatu UNESPDepartment of Surgery and Veterinary Anesthesiology School of Veterinary Medicine and Animal Science UNESPDepartment of Biostatistics Institute of Biosciences of Botucatu UNESPUniversidade Estadual Paulista (Unesp)University of Saõ PauloGushiken, Lucas Fernando Sérgio [UNESP]Hussni, Carlos Alberto [UNESP]Bastos, Jairo KenuppRozza, Ariane Leite [UNESP]Beserra, Fernando Pereira [UNESP]Vieira, Ana Júlia [UNESP]Padovani, Carlos Roberto [UNESP]Lemos, MarivanePolizello Junior, MaurilioSilva, Jonas Joaquim Mangabeira DaNóbrega, Rafael Henrique [UNESP]Martinez, Emanuel Ricardo Monteiro [UNESP]Pellizzon, Claúdia Helena [UNESP]2018-12-11T17:14:58Z2018-12-11T17:14:58Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2017/6589270Evidence-based Complementary and Alternative Medicine, v. 2017.1741-42881741-427Xhttp://hdl.handle.net/11449/17524310.1155/2017/65892702-s2.0-850298131702-s2.0-85029813170.pdf051570858525398500193937798010690000-0001-9796-50760000-0002-4494-4180Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicine0,683info:eu-repo/semantics/openAccess2023-11-17T06:16:29Zoai:repositorio.unesp.br:11449/175243Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-17T06:16:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats |
title |
Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats |
spellingShingle |
Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats Gushiken, Lucas Fernando Sérgio [UNESP] |
title_short |
Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats |
title_full |
Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats |
title_fullStr |
Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats |
title_full_unstemmed |
Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats |
title_sort |
Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats |
author |
Gushiken, Lucas Fernando Sérgio [UNESP] |
author_facet |
Gushiken, Lucas Fernando Sérgio [UNESP] Hussni, Carlos Alberto [UNESP] Bastos, Jairo Kenupp Rozza, Ariane Leite [UNESP] Beserra, Fernando Pereira [UNESP] Vieira, Ana Júlia [UNESP] Padovani, Carlos Roberto [UNESP] Lemos, Marivane Polizello Junior, Maurilio Silva, Jonas Joaquim Mangabeira Da Nóbrega, Rafael Henrique [UNESP] Martinez, Emanuel Ricardo Monteiro [UNESP] Pellizzon, Claúdia Helena [UNESP] |
author_role |
author |
author2 |
Hussni, Carlos Alberto [UNESP] Bastos, Jairo Kenupp Rozza, Ariane Leite [UNESP] Beserra, Fernando Pereira [UNESP] Vieira, Ana Júlia [UNESP] Padovani, Carlos Roberto [UNESP] Lemos, Marivane Polizello Junior, Maurilio Silva, Jonas Joaquim Mangabeira Da Nóbrega, Rafael Henrique [UNESP] Martinez, Emanuel Ricardo Monteiro [UNESP] Pellizzon, Claúdia Helena [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Saõ Paulo |
dc.contributor.author.fl_str_mv |
Gushiken, Lucas Fernando Sérgio [UNESP] Hussni, Carlos Alberto [UNESP] Bastos, Jairo Kenupp Rozza, Ariane Leite [UNESP] Beserra, Fernando Pereira [UNESP] Vieira, Ana Júlia [UNESP] Padovani, Carlos Roberto [UNESP] Lemos, Marivane Polizello Junior, Maurilio Silva, Jonas Joaquim Mangabeira Da Nóbrega, Rafael Henrique [UNESP] Martinez, Emanuel Ricardo Monteiro [UNESP] Pellizzon, Claúdia Helena [UNESP] |
description |
The wound healing is a complex process which, sometimes, can be a problem in public health because of the possibility of physical disability or even death. Due to the lack of a gold standard drug in skin wound treatment and aiming at the discovery of new treatments in skin repair and the mechanisms involved in the process, we used oleoresin (OR) from Copaifera langsdorffii and hydroalcoholic extract of the leaves (EH) to treat rat skin wounds. For that, male Wistar rats were divided into groups (n=8): Lanette, Collagenase, 10% EH, or 10% OR and, after anesthesia, one wound of 2 cm was made in the back of animals. The wounds were treated once a day for 3, 7, or 14 days and the wound areas were measured. The rats were euthanized and skin samples destined to biochemical, molecular, and immunohistochemical analysis. The results showed a macroscopic retraction of the wounds of 10% EH and 10% OR creams and both treatments showed anti-inflammatory activity. Molecular and immunohistochemical results demonstrated the activity of Copaifera langsdorffii creams in angiogenesis, reepithelialization, wound retraction, and remodeling mechanisms. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:14:58Z 2018-12-11T17:14:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2017/6589270 Evidence-based Complementary and Alternative Medicine, v. 2017. 1741-4288 1741-427X http://hdl.handle.net/11449/175243 10.1155/2017/6589270 2-s2.0-85029813170 2-s2.0-85029813170.pdf 0515708585253985 0019393779801069 0000-0001-9796-5076 0000-0002-4494-4180 |
url |
http://dx.doi.org/10.1155/2017/6589270 http://hdl.handle.net/11449/175243 |
identifier_str_mv |
Evidence-based Complementary and Alternative Medicine, v. 2017. 1741-4288 1741-427X 10.1155/2017/6589270 2-s2.0-85029813170 2-s2.0-85029813170.pdf 0515708585253985 0019393779801069 0000-0001-9796-5076 0000-0002-4494-4180 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Evidence-based Complementary and Alternative Medicine 0,683 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964975454748672 |