X-ray spectra in SEM and staining with chrome azurol S show Al deposits in leaf tissues of Al-accumulating and non-accumulating plants from the cerrado
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11104-016-2841-1 http://hdl.handle.net/11449/177860 |
Resumo: | Aims: Aluminum (Al) accumulating plants are distributed throughout the world. In the Cerrado, Al-accumulating and non-accumulating species coexist. Therefore, we anatomically/histochemically explore the sites of Al deposits in Al-accumulating species, and we also ask whether Al can be observed in non-accumulating species. Methods: The anatomical patterns of Al storage in leaf tissues of Al-accumulating [Miconia albicans, M. rubiginosa (Melastomataceae), Qualea grandiflora, and Q. parviflora (Vochysiaceae)] and non-accumulating species [(Styrax ferrugineus and S. camporum (Styracaceae)] were described using different Al indicator dyes: hematoxylin and chrome azurol S (CAS). In addition, Al-specific x-ray spectra from different regions of leaf tissues were measured and analyzed by scanning electron microscopy (SEM). Results: When compared to hematoxylin, it was confirmed by x-ray spectra in SEM that CAS was a more contrasting indicator of Al presence. Silica granules associated to Al were observed on cell walls of non-lignified leaf tissues of Al-accumulating species. However, granules were also found in leaf midribs of S. camporum. Conclusions: The anatomical description of Al accumulation in leaves and the consistent pattern of Al association with cell walls strongly suggest that Al has structural rather than physiological roles in leaves of Cerrado woody plants, and that Al is perhaps isolated from metabolism. |
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X-ray spectra in SEM and staining with chrome azurol S show Al deposits in leaf tissues of Al-accumulating and non-accumulating plants from the cerradoAl3+Brazilian savannaHistochemical studiesMelastomataceaeStyracaceaeVochysiaceaeAims: Aluminum (Al) accumulating plants are distributed throughout the world. In the Cerrado, Al-accumulating and non-accumulating species coexist. Therefore, we anatomically/histochemically explore the sites of Al deposits in Al-accumulating species, and we also ask whether Al can be observed in non-accumulating species. Methods: The anatomical patterns of Al storage in leaf tissues of Al-accumulating [Miconia albicans, M. rubiginosa (Melastomataceae), Qualea grandiflora, and Q. parviflora (Vochysiaceae)] and non-accumulating species [(Styrax ferrugineus and S. camporum (Styracaceae)] were described using different Al indicator dyes: hematoxylin and chrome azurol S (CAS). In addition, Al-specific x-ray spectra from different regions of leaf tissues were measured and analyzed by scanning electron microscopy (SEM). Results: When compared to hematoxylin, it was confirmed by x-ray spectra in SEM that CAS was a more contrasting indicator of Al presence. Silica granules associated to Al were observed on cell walls of non-lignified leaf tissues of Al-accumulating species. However, granules were also found in leaf midribs of S. camporum. Conclusions: The anatomical description of Al accumulation in leaves and the consistent pattern of Al association with cell walls strongly suggest that Al has structural rather than physiological roles in leaves of Cerrado woody plants, and that Al is perhaps isolated from metabolism.Programa de Pós-Graduação em Ciências Biológicas (Biologia Vegetal) Instituto de Biociências Departamento de Botânica Univ Estadual Paulista Unesp, Av. 24-A, 1515Instituto de Biociências Departamento de Botânica Univ Estadual Paulista Unesp, Av. 24-A, 1515Programa de Pós-Graduação em Ciências Biológicas (Biologia Vegetal) Instituto de Biociências Departamento de Botânica Univ Estadual Paulista Unesp, Av. 24-A, 1515Instituto de Biociências Departamento de Botânica Univ Estadual Paulista Unesp, Av. 24-A, 1515Universidade Estadual Paulista (Unesp)Bressan, Anna C. G. [UNESP]Coan, Alessandra I. [UNESP]Habermann, Gustavo [UNESP]2018-12-11T17:27:27Z2018-12-11T17:27:27Z2016-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article293-306application/pdfhttp://dx.doi.org/10.1007/s11104-016-2841-1Plant and Soil, v. 404, n. 1-2, p. 293-306, 2016.1573-50360032-079Xhttp://hdl.handle.net/11449/17786010.1007/s11104-016-2841-12-s2.0-849595487692-s2.0-84959548769.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant and Soil1,361info:eu-repo/semantics/openAccess2023-10-15T06:07:51Zoai:repositorio.unesp.br:11449/177860Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-15T06:07:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
X-ray spectra in SEM and staining with chrome azurol S show Al deposits in leaf tissues of Al-accumulating and non-accumulating plants from the cerrado |
title |
X-ray spectra in SEM and staining with chrome azurol S show Al deposits in leaf tissues of Al-accumulating and non-accumulating plants from the cerrado |
spellingShingle |
X-ray spectra in SEM and staining with chrome azurol S show Al deposits in leaf tissues of Al-accumulating and non-accumulating plants from the cerrado Bressan, Anna C. G. [UNESP] Al3+ Brazilian savanna Histochemical studies Melastomataceae Styracaceae Vochysiaceae |
title_short |
X-ray spectra in SEM and staining with chrome azurol S show Al deposits in leaf tissues of Al-accumulating and non-accumulating plants from the cerrado |
title_full |
X-ray spectra in SEM and staining with chrome azurol S show Al deposits in leaf tissues of Al-accumulating and non-accumulating plants from the cerrado |
title_fullStr |
X-ray spectra in SEM and staining with chrome azurol S show Al deposits in leaf tissues of Al-accumulating and non-accumulating plants from the cerrado |
title_full_unstemmed |
X-ray spectra in SEM and staining with chrome azurol S show Al deposits in leaf tissues of Al-accumulating and non-accumulating plants from the cerrado |
title_sort |
X-ray spectra in SEM and staining with chrome azurol S show Al deposits in leaf tissues of Al-accumulating and non-accumulating plants from the cerrado |
author |
Bressan, Anna C. G. [UNESP] |
author_facet |
Bressan, Anna C. G. [UNESP] Coan, Alessandra I. [UNESP] Habermann, Gustavo [UNESP] |
author_role |
author |
author2 |
Coan, Alessandra I. [UNESP] Habermann, Gustavo [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bressan, Anna C. G. [UNESP] Coan, Alessandra I. [UNESP] Habermann, Gustavo [UNESP] |
dc.subject.por.fl_str_mv |
Al3+ Brazilian savanna Histochemical studies Melastomataceae Styracaceae Vochysiaceae |
topic |
Al3+ Brazilian savanna Histochemical studies Melastomataceae Styracaceae Vochysiaceae |
description |
Aims: Aluminum (Al) accumulating plants are distributed throughout the world. In the Cerrado, Al-accumulating and non-accumulating species coexist. Therefore, we anatomically/histochemically explore the sites of Al deposits in Al-accumulating species, and we also ask whether Al can be observed in non-accumulating species. Methods: The anatomical patterns of Al storage in leaf tissues of Al-accumulating [Miconia albicans, M. rubiginosa (Melastomataceae), Qualea grandiflora, and Q. parviflora (Vochysiaceae)] and non-accumulating species [(Styrax ferrugineus and S. camporum (Styracaceae)] were described using different Al indicator dyes: hematoxylin and chrome azurol S (CAS). In addition, Al-specific x-ray spectra from different regions of leaf tissues were measured and analyzed by scanning electron microscopy (SEM). Results: When compared to hematoxylin, it was confirmed by x-ray spectra in SEM that CAS was a more contrasting indicator of Al presence. Silica granules associated to Al were observed on cell walls of non-lignified leaf tissues of Al-accumulating species. However, granules were also found in leaf midribs of S. camporum. Conclusions: The anatomical description of Al accumulation in leaves and the consistent pattern of Al association with cell walls strongly suggest that Al has structural rather than physiological roles in leaves of Cerrado woody plants, and that Al is perhaps isolated from metabolism. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-01 2018-12-11T17:27:27Z 2018-12-11T17:27:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11104-016-2841-1 Plant and Soil, v. 404, n. 1-2, p. 293-306, 2016. 1573-5036 0032-079X http://hdl.handle.net/11449/177860 10.1007/s11104-016-2841-1 2-s2.0-84959548769 2-s2.0-84959548769.pdf |
url |
http://dx.doi.org/10.1007/s11104-016-2841-1 http://hdl.handle.net/11449/177860 |
identifier_str_mv |
Plant and Soil, v. 404, n. 1-2, p. 293-306, 2016. 1573-5036 0032-079X 10.1007/s11104-016-2841-1 2-s2.0-84959548769 2-s2.0-84959548769.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plant and Soil 1,361 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
293-306 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046005995732992 |