Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytes
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2023.107262 http://hdl.handle.net/11449/248951 |
Resumo: | In this study, we aimed to propose changes in the protocol of cultured Astyanax altiparanae hypophysation to increase the maximum ovulation rate of 60% registered previously. To that two consecutive experiments were conducted. In the first experiment, three carp pituitary homogenate (CPH) doses (3, 6, and 9 mg/kg) were administered in a single injection, while in the second experiment, the 6 mg/kg CPH dose was tested either in single or double injections. In the first experiment, a single injection of 3 mg/kg CPH did not induce final oocyte maturation or spawning, while a dose of 6 mg/kg CPH resulted in an increase in the plasma level of prostaglandin (PGF2α) at ovulation. The single higher dose of 9 mg/kg CPH did not improve reproductive performance and even though anticipated the resumption of meiosis it was detrimental to the spawning rate. In the second experiment, the dose of 6 mg/kg CPH fractionated into two injections led to a higher spawning rate, spawning volume per female body mass, frequency of post-ovulatory complexes, and PGF2α concentration at ovulation compared to the single injection. The most effective treatment remained the 6 mg/kg of CPH fractionated into two injections, but still providing very low proportion of ovulated females (∼40 %). Overall, this study indicates that the spawning protocols for this species need to be improved to induce ovulation in a larger number of females and be more potent in those females that respond positively. |
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Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytesHormonal inductionHyphophysationLambariPGF2αProstaglandinIn this study, we aimed to propose changes in the protocol of cultured Astyanax altiparanae hypophysation to increase the maximum ovulation rate of 60% registered previously. To that two consecutive experiments were conducted. In the first experiment, three carp pituitary homogenate (CPH) doses (3, 6, and 9 mg/kg) were administered in a single injection, while in the second experiment, the 6 mg/kg CPH dose was tested either in single or double injections. In the first experiment, a single injection of 3 mg/kg CPH did not induce final oocyte maturation or spawning, while a dose of 6 mg/kg CPH resulted in an increase in the plasma level of prostaglandin (PGF2α) at ovulation. The single higher dose of 9 mg/kg CPH did not improve reproductive performance and even though anticipated the resumption of meiosis it was detrimental to the spawning rate. In the second experiment, the dose of 6 mg/kg CPH fractionated into two injections led to a higher spawning rate, spawning volume per female body mass, frequency of post-ovulatory complexes, and PGF2α concentration at ovulation compared to the single injection. The most effective treatment remained the 6 mg/kg of CPH fractionated into two injections, but still providing very low proportion of ovulated females (∼40 %). Overall, this study indicates that the spawning protocols for this species need to be improved to induce ovulation in a larger number of females and be more potent in those females that respond positively.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Centro de Aquicultura da UNESP – CAUNESP Universidade Estadual Paulista – UNESP, Via de Acesso Prof. Paulo Donato Castellane, S/N, SPCentro de Aquicultura da UNESP – CAUNESP Universidade Estadual Paulista – UNESP, Via de Acesso Prof. Paulo Donato Castellane, S/N, SPFAPESP: #2016/25988–7CAPES: 001Universidade Estadual Paulista (UNESP)Ariki, Daniel Guimarães Figueiredo [UNESP]Roza de Abreu, Mariana [UNESP]de Jesus Silva, Laíza Maria [UNESP]Sato, Rafael Tomoda [UNESP]Batlouni, Sergio Ricardo [UNESP]2023-07-29T13:58:16Z2023-07-29T13:58:16Z2023-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.anireprosci.2023.107262Animal Reproduction Science, v. 254.0378-4320http://hdl.handle.net/11449/24895110.1016/j.anireprosci.2023.1072622-s2.0-85161286751Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2024-04-09T15:36:42Zoai:repositorio.unesp.br:11449/248951Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:16:43.436726Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytes |
title |
Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytes |
spellingShingle |
Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytes Ariki, Daniel Guimarães Figueiredo [UNESP] Hormonal induction Hyphophysation Lambari PGF2α Prostaglandin |
title_short |
Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytes |
title_full |
Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytes |
title_fullStr |
Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytes |
title_full_unstemmed |
Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytes |
title_sort |
Attempts for increasing Astyanax altiparanae spawning rates and percentage of responsive oocytes |
author |
Ariki, Daniel Guimarães Figueiredo [UNESP] |
author_facet |
Ariki, Daniel Guimarães Figueiredo [UNESP] Roza de Abreu, Mariana [UNESP] de Jesus Silva, Laíza Maria [UNESP] Sato, Rafael Tomoda [UNESP] Batlouni, Sergio Ricardo [UNESP] |
author_role |
author |
author2 |
Roza de Abreu, Mariana [UNESP] de Jesus Silva, Laíza Maria [UNESP] Sato, Rafael Tomoda [UNESP] Batlouni, Sergio Ricardo [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Ariki, Daniel Guimarães Figueiredo [UNESP] Roza de Abreu, Mariana [UNESP] de Jesus Silva, Laíza Maria [UNESP] Sato, Rafael Tomoda [UNESP] Batlouni, Sergio Ricardo [UNESP] |
dc.subject.por.fl_str_mv |
Hormonal induction Hyphophysation Lambari PGF2α Prostaglandin |
topic |
Hormonal induction Hyphophysation Lambari PGF2α Prostaglandin |
description |
In this study, we aimed to propose changes in the protocol of cultured Astyanax altiparanae hypophysation to increase the maximum ovulation rate of 60% registered previously. To that two consecutive experiments were conducted. In the first experiment, three carp pituitary homogenate (CPH) doses (3, 6, and 9 mg/kg) were administered in a single injection, while in the second experiment, the 6 mg/kg CPH dose was tested either in single or double injections. In the first experiment, a single injection of 3 mg/kg CPH did not induce final oocyte maturation or spawning, while a dose of 6 mg/kg CPH resulted in an increase in the plasma level of prostaglandin (PGF2α) at ovulation. The single higher dose of 9 mg/kg CPH did not improve reproductive performance and even though anticipated the resumption of meiosis it was detrimental to the spawning rate. In the second experiment, the dose of 6 mg/kg CPH fractionated into two injections led to a higher spawning rate, spawning volume per female body mass, frequency of post-ovulatory complexes, and PGF2α concentration at ovulation compared to the single injection. The most effective treatment remained the 6 mg/kg of CPH fractionated into two injections, but still providing very low proportion of ovulated females (∼40 %). Overall, this study indicates that the spawning protocols for this species need to be improved to induce ovulation in a larger number of females and be more potent in those females that respond positively. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:58:16Z 2023-07-29T13:58:16Z 2023-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2023.107262 Animal Reproduction Science, v. 254. 0378-4320 http://hdl.handle.net/11449/248951 10.1016/j.anireprosci.2023.107262 2-s2.0-85161286751 |
url |
http://dx.doi.org/10.1016/j.anireprosci.2023.107262 http://hdl.handle.net/11449/248951 |
identifier_str_mv |
Animal Reproduction Science, v. 254. 0378-4320 10.1016/j.anireprosci.2023.107262 2-s2.0-85161286751 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129046286958592 |