Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-019-38536-4 http://hdl.handle.net/11449/190129 |
Resumo: | The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth. |
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Repositório Institucional da UNESP |
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spelling |
Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus)The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth.São Paulo State University (UNESP) Institute of Biosciences Department of MorphologyUniversity of West São Paulo (UNOESTE)São Paulo State University (UNESP) Institute of Biosciences Department of Chemistry and BiochemistrySão Paulo State University (UNESP) Institute of Biosciences Department of MorphologySão Paulo State University (UNESP) Institute of Biosciences Department of Chemistry and BiochemistryUniversidade Estadual Paulista (Unesp)University of West São Paulo (UNOESTE)Duran, Bruno Oliveira Silva [UNESP]Góes, Guilherme Alcarás [UNESP]Zanella, Bruna Tereza Thomazini [UNESP]Freire, Paula Paccielli [UNESP]Valente, Jessica Silvino [UNESP]Salomão, Rondinelle Artur SimõesFernandes, Ana [UNESP]Mareco, Edson AssunçãoCarvalho, Robson Francisco [UNESP]Dal-Pai-Silva, Maeli [UNESP]2019-10-06T17:03:15Z2019-10-06T17:03:15Z2019-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-019-38536-4Scientific Reports, v. 9, n. 1, 2019.2045-2322http://hdl.handle.net/11449/19012910.1038/s41598-019-38536-42-s2.0-85061759873Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-23T04:23:37Zoai:repositorio.unesp.br:11449/190129Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:10:07.597200Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title |
Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
spellingShingle |
Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) Duran, Bruno Oliveira Silva [UNESP] |
title_short |
Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title_full |
Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title_fullStr |
Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title_full_unstemmed |
Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
title_sort |
Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus) |
author |
Duran, Bruno Oliveira Silva [UNESP] |
author_facet |
Duran, Bruno Oliveira Silva [UNESP] Góes, Guilherme Alcarás [UNESP] Zanella, Bruna Tereza Thomazini [UNESP] Freire, Paula Paccielli [UNESP] Valente, Jessica Silvino [UNESP] Salomão, Rondinelle Artur Simões Fernandes, Ana [UNESP] Mareco, Edson Assunção Carvalho, Robson Francisco [UNESP] Dal-Pai-Silva, Maeli [UNESP] |
author_role |
author |
author2 |
Góes, Guilherme Alcarás [UNESP] Zanella, Bruna Tereza Thomazini [UNESP] Freire, Paula Paccielli [UNESP] Valente, Jessica Silvino [UNESP] Salomão, Rondinelle Artur Simões Fernandes, Ana [UNESP] Mareco, Edson Assunção Carvalho, Robson Francisco [UNESP] Dal-Pai-Silva, Maeli [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of West São Paulo (UNOESTE) |
dc.contributor.author.fl_str_mv |
Duran, Bruno Oliveira Silva [UNESP] Góes, Guilherme Alcarás [UNESP] Zanella, Bruna Tereza Thomazini [UNESP] Freire, Paula Paccielli [UNESP] Valente, Jessica Silvino [UNESP] Salomão, Rondinelle Artur Simões Fernandes, Ana [UNESP] Mareco, Edson Assunção Carvalho, Robson Francisco [UNESP] Dal-Pai-Silva, Maeli [UNESP] |
description |
The postembryonic growth of skeletal muscle in teleost fish involves myoblast proliferation, migration and differentiation, encompassing the main events of embryonic myogenesis. Ascorbic acid plays important cellular and biochemical roles as an antioxidant and contributes to the proper collagen biosynthesis necessary for the structure of connective and bone tissues. However, whether ascorbic acid can directly influence the mechanisms of fish myogenesis and skeletal muscle growth remains unclear. The aim of our work was to evaluate the effects of ascorbic acid supplementation on the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). To provide insight into the potential antioxidant role of ascorbic acid, we also treated myoblasts in vitro with menadione, which is a powerful oxidant. Our results show that ascorbic acid-supplemented myoblasts exhibit increased proliferation and migration and are protected against the oxidative stress caused by menadione. In addition, ascorbic acid increased the activity of the antioxidant enzyme superoxide dismutase and the expression of myog and mtor, which are molecular markers related to skeletal muscle myogenesis and protein synthesis, respectively. This work reveals a direct influence of ascorbic acid on the mechanisms of pacu myogenesis and highlights the potential use of ascorbic acid for stimulating fish skeletal muscle growth. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:03:15Z 2019-10-06T17:03:15Z 2019-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-019-38536-4 Scientific Reports, v. 9, n. 1, 2019. 2045-2322 http://hdl.handle.net/11449/190129 10.1038/s41598-019-38536-4 2-s2.0-85061759873 |
url |
http://dx.doi.org/10.1038/s41598-019-38536-4 http://hdl.handle.net/11449/190129 |
identifier_str_mv |
Scientific Reports, v. 9, n. 1, 2019. 2045-2322 10.1038/s41598-019-38536-4 2-s2.0-85061759873 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129292805079040 |