Quantification of adhesion of mesenchymal stem cells spread on decellularized vein scaffold

• Autor(a) principal: Rodrigues, Lenize da Silva [UNESP]
• Data de Publicação: 2021
• Outros Autores: Bovolato, Ana Lívia de Carvalho [UNESP], Silva, Bárbara Esteves [UNESP], Chizzolini, Leticia Victória [UNESP], da Cruz, Bianca Latance [UNESP], Moraes, Marcelo Padovani de Toledo [UNESP], Lourenção, Pedro Luiz Toledo de Arruda [UNESP], Bertanha, Matheus [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/ACB361001; http://hdl.handle.net/11449/229869
• topic: Blood vessels; Endothelium; Mesenchymal stem cells; Peripheral arterial disease

Purpose: To evaluate methods that improve adipose-derived stem cells (ASCs) population in decellularized biological venous scaffold for tissue engineering in blood vessels, a model in rabbits. Methods: The ASC was expanded until the third passage. Inferior vena cava (IVC) was submitted to the decellularization process using 1% sodium dodecyl sulfate (SDS) or 2% sodium deoxycholate (SD) to compose 12 study groups (G): pure SD or SDS, exposed or not to 1% TritonX-100 (TX-100) and exposed or not to poly-l’lysine and laminin (PL). Scaffolds were covered with 1 × 105 or 1 × 106 ASCs diluted in 10 μL Puramatrix™. The histological analysis was done by cell counting in hematoxylin and eosin (HE) and nuclei count in immunofluorescence (IF) with 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI). Results: The study of groups in HE and IF showed similar results. For both analyses, IVC-SD-1 × 106 ASC and IVC-SD-PL-1 × 106 ASC provided the best results. The IF technique showed better sensitivity than HE, with a weak agreement between them. Conclusion: Decellularizing agent and the number of ASC influence scaffolds cellularization response and the best protocols as those ones using SD with or without the addition of PL.