Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.procbio.2020.07.002 http://hdl.handle.net/11449/201935 |
Resumo: | This paper presents a study on scale-up and cost reduction of the production of spores of Metarhizium anisopliae IBCB 425, entomopathogenic fungus used in sugarcane crops. Rice was mixed with sugarcane bagasse (9:1 w/w) for substrate composition, assuring adequate physical structure for cultivation in packed-beds. Spores yield from only rice in bench-scale packed-bed bioreactor was 56 % of the one obtained from the mixture 9:1 w/w. In comparison to plastic packages used in bioindustries, equivalent spores yields per gram of substrate have been achieved in bench and pilot-scale bioreactors built by cylindrical jacketed modules, that provide better control of operational and environmental variables, attested by little variability among replicates. Although non-negligible temperature rise (5 °C above the ideal) occurred within the pilot-scale bioreactor, spores production was not harmed in comparison to bench-scale. By reusing rice up to three successive cultivations, a 2.5-fold increase of spores yields was achieved in comparison to single use. Temperatures and CO2 profiles corroborates the fungus adapted differently to substrate at each usage. Such results are valuable for industrial producers of commercial formulations of the fungus spores, allowing process modernization by using packed-bed bioreactors and production costs and rice demand reduction by recycling the substrate. |
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Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivationsBiocontrolScale-upSolid-state fermentationSugarcane cropsThis paper presents a study on scale-up and cost reduction of the production of spores of Metarhizium anisopliae IBCB 425, entomopathogenic fungus used in sugarcane crops. Rice was mixed with sugarcane bagasse (9:1 w/w) for substrate composition, assuring adequate physical structure for cultivation in packed-beds. Spores yield from only rice in bench-scale packed-bed bioreactor was 56 % of the one obtained from the mixture 9:1 w/w. In comparison to plastic packages used in bioindustries, equivalent spores yields per gram of substrate have been achieved in bench and pilot-scale bioreactors built by cylindrical jacketed modules, that provide better control of operational and environmental variables, attested by little variability among replicates. Although non-negligible temperature rise (5 °C above the ideal) occurred within the pilot-scale bioreactor, spores production was not harmed in comparison to bench-scale. By reusing rice up to three successive cultivations, a 2.5-fold increase of spores yields was achieved in comparison to single use. Temperatures and CO2 profiles corroborates the fungus adapted differently to substrate at each usage. Such results are valuable for industrial producers of commercial formulations of the fungus spores, allowing process modernization by using packed-bed bioreactors and production costs and rice demand reduction by recycling the substrate.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Graduate Program in Food Engineering and Science Food Engineering and Technology Department Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo 2265, Jardim NazarethGraduate Program in Chemical Engineering Chemical Engineering Department Center of Exact Sciences and of Technology Federal University of São Carlos (UFSCar), Rod. Washington Luiz km 235 SP 310, Bairro MonjolinhoGraduate Program in Food Engineering and Science Food Engineering and Technology Department Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo 2265, Jardim NazarethFAPESP: 2010/12624-0FAPESP: 2014/23453-3FAPESP: 2018/00996-2CNPq: 430786/2018-2Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)da Cunha, Lucas Portilho [UNESP]Casciatori, Fernanda PerpétuaVicente, Isabela Valente [UNESP]Garcia, Renata Laurito [UNESP]Thoméo, João Cláudio [UNESP]2020-12-12T02:45:40Z2020-12-12T02:45:40Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article104-111http://dx.doi.org/10.1016/j.procbio.2020.07.002Process Biochemistry, v. 97, p. 104-111.1359-5113http://hdl.handle.net/11449/20193510.1016/j.procbio.2020.07.0022-s2.0-85087589803Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProcess Biochemistryinfo:eu-repo/semantics/openAccess2021-10-23T03:12:44Zoai:repositorio.unesp.br:11449/201935Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T11:08:03.589007Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations |
title |
Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations |
spellingShingle |
Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations da Cunha, Lucas Portilho [UNESP] Biocontrol Scale-up Solid-state fermentation Sugarcane crops |
title_short |
Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations |
title_full |
Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations |
title_fullStr |
Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations |
title_full_unstemmed |
Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations |
title_sort |
Metarhizium anisopliae conidia production in packed-bed bioreactor using rice as substrate in successive cultivations |
author |
da Cunha, Lucas Portilho [UNESP] |
author_facet |
da Cunha, Lucas Portilho [UNESP] Casciatori, Fernanda Perpétua Vicente, Isabela Valente [UNESP] Garcia, Renata Laurito [UNESP] Thoméo, João Cláudio [UNESP] |
author_role |
author |
author2 |
Casciatori, Fernanda Perpétua Vicente, Isabela Valente [UNESP] Garcia, Renata Laurito [UNESP] Thoméo, João Cláudio [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
da Cunha, Lucas Portilho [UNESP] Casciatori, Fernanda Perpétua Vicente, Isabela Valente [UNESP] Garcia, Renata Laurito [UNESP] Thoméo, João Cláudio [UNESP] |
dc.subject.por.fl_str_mv |
Biocontrol Scale-up Solid-state fermentation Sugarcane crops |
topic |
Biocontrol Scale-up Solid-state fermentation Sugarcane crops |
description |
This paper presents a study on scale-up and cost reduction of the production of spores of Metarhizium anisopliae IBCB 425, entomopathogenic fungus used in sugarcane crops. Rice was mixed with sugarcane bagasse (9:1 w/w) for substrate composition, assuring adequate physical structure for cultivation in packed-beds. Spores yield from only rice in bench-scale packed-bed bioreactor was 56 % of the one obtained from the mixture 9:1 w/w. In comparison to plastic packages used in bioindustries, equivalent spores yields per gram of substrate have been achieved in bench and pilot-scale bioreactors built by cylindrical jacketed modules, that provide better control of operational and environmental variables, attested by little variability among replicates. Although non-negligible temperature rise (5 °C above the ideal) occurred within the pilot-scale bioreactor, spores production was not harmed in comparison to bench-scale. By reusing rice up to three successive cultivations, a 2.5-fold increase of spores yields was achieved in comparison to single use. Temperatures and CO2 profiles corroborates the fungus adapted differently to substrate at each usage. Such results are valuable for industrial producers of commercial formulations of the fungus spores, allowing process modernization by using packed-bed bioreactors and production costs and rice demand reduction by recycling the substrate. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:45:40Z 2020-12-12T02:45:40Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.procbio.2020.07.002 Process Biochemistry, v. 97, p. 104-111. 1359-5113 http://hdl.handle.net/11449/201935 10.1016/j.procbio.2020.07.002 2-s2.0-85087589803 |
url |
http://dx.doi.org/10.1016/j.procbio.2020.07.002 http://hdl.handle.net/11449/201935 |
identifier_str_mv |
Process Biochemistry, v. 97, p. 104-111. 1359-5113 10.1016/j.procbio.2020.07.002 2-s2.0-85087589803 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Process Biochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
104-111 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045774246805504 |