Estudo químico e atividade antirradicalar da fração acetato de etila das folhas de Actinocephalus divaricatus (Koern.) Sano (Eriocaulaceae)

• Autor(a) principal: Souza, Thaís Pezza de [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155540; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-06-19/000885548.pdf
• topic: Ácido fenólico; Eriocaulaceae; Metabólitos; Flavonoides; Cromatografia líquida de alta eficiência; Flavonoids

The Eriocaulaceae family has 1.400 species divided into 10 genera, with many plants being endemic in Brazil. One of these genera is the Actinocephalus, which until 2004 was a subsection of the genre Paepalanthus and, because of cladistic studies and morphological analyzes of several species, was elevated to genus Actinocephalus. Therefore this genus currently has 49 species and the specie studied in this work was Actinocephalus divaricatus. The aerial parts of A. divaricatus were separated into scapes, capitula and leaves. A methanolic extract was made with the leaves, and after a partition with wather and ethyl acetate was made and with the aqueous fraction a partition with n-butanol was made. The ethyl acetate fraction was studied, mainly due to its high yield, and then a fractioning in sephadex LH-20 was made. With the obtained fractions was made a TLC, providing the fraction 24 (1,5 mg) that, by presenting only one spot, was analyzed by NMR of 1H, and was identified like 6-hydroxy-7-methoxiquecertin (AdFa1). The fraction 12 (129,5 mg) was also obtained and was purified and separated by HPLC-PDA preparative, resulting in four other substances that, after analysis, were identified like being: 3,4-dihydroxybenzoic acid (AdFa2), p-hydroxybenzoic acid (AdFa3) e 4-hydroxy-3-methoxybenzoic acid (AdFa4) and a flavonoid derivated from quercetin: 6-hydroxy-7-methoxyquercetin-3-O-β-D-glicopyranoside (AdFa5). With AdFa5 was made the quantification by HPLC-PDA, being determined in the sample 25,65 ± 0,43 mg g-1 of flavonoid (AdFa5) in the ethyl acetate fraction. In addition, the antiradicalar test was performed using standard gallic acid, which showed IC50 of 30,69 ± 4,9 μg mL-1, and with the standart quercetin, it showed a IC50 of 50,96 ± 5,6 μg mL-1, while the fraction IC50 was 440,66 ± 24,27 μg mL-1. The total phenol content in the ethyl acetate fraction was 274,61 μg mg-1, while the total flavonoids content was 13,14 μg mg-1 of ethyl...